LOCUS       sequence-248698-        4311 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes human E-Cadherin as a Type 3 part to be used in the MTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3_035
FEATURES             Location/Qualifiers
     source          1..4311
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     366..385
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3242..3276)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(3347..4006)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3921..3940
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(4188..4311,1..465)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
       61 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      121 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      181 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      241 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      301 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
      361 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
      421 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      481 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
      541 tcccctgatt ctgtggataa ccgtagtcgg tctcatatgg gcccttggag ccgcagcctc
      601 tcggcgctgc tgctgctgct gcaagtctcc tcttggctct gccaggagcc ggagccctgc
      661 caccctggct ttgacgccga gagctacacg ttcacggtgc cccggcgcca cctggagaga
      721 ggccgcgtcc tgggcagagt gaattttgaa gattgcaccg gtcgacaaag gacagcctat
      781 ttttccctcg acacccgatt caaagtgggc acagatggtg tgattacagt caaaaggcct
      841 ctacggtttc ataacccaca gatccatttc ttggtctacg cctgggactc cacctacaga
      901 aagttttcca ccaaagtcac gctgaataca gtggggcacc accaccgccc cccgccccat
      961 caggcctccg tttctggaat ccaagcagaa ttgctcacat ttcccaactc ctctcctggc
     1021 ctcagaagac agaagagaga ctgggttatt cctcccatca gctgcccaga aaatgaaaaa
     1081 ggcccatttc ctaaaaacct ggttcagatc aaatccaaca aagacaaaga aggcaaggtt
     1141 ttctacagca tcactggcca aggagctgac acaccccctg ttggtgtctt tattattgaa
     1201 agagaaacag gatggctgaa ggtgacagag cctctggata gagaacgcat tgccacatac
     1261 actctcttct ctcacgctgt gtcatccaac gggaatgcag ttgaggatcc aatggagatt
     1321 ttgatcacgg taaccgatca gaatgacaac aagcccgaat tcacccagga ggtctttaag
     1381 gggtctgtca tggaaggtgc tcttccagga acctctgtga tggaggtcac agccacagac
     1441 gcggacgatg atgtgaacac ctacaatgcc gccatcgctt acaccatcct cagccaagat
     1501 cctgagctcc ctgacaaaaa tatgttcacc attaacagga acacaggagt catcagtgtg
     1561 gtcaccactg ggctggaccg agagagtttc cctacgtata ccctggtggt tcaagctgct
     1621 gaccttcaag gtgaggggtt aagcacaaca gcaacagctg tgatcacagt cactgacacc
     1681 aacgataatc ctccgatctt caatcccacc acgtacaagg gtcaggtgcc tgagaacgag
     1741 gctaacgtcg taatcaccac actgaaagtg actgatgctg atgcccccaa taccccagcg
     1801 tgggaggctg tatacaccat attgaatgat gatggtggac aatttgtcgt caccacaaat
     1861 ccagtgaaca acgatggcat tttgaaaaca gcaaagggct tggattttga ggccaagcag
     1921 cagtacattc tacacgtagc agtgacgaat gtggtacctt ttgaagtctc tctcaccacc
     1981 tccacagcca ccgtcaccgt ggatgtgctg gatgtgaatg aagcccccat ctttgtgcct
     2041 cctgaaaaga gagtggaagt gtccgaggac tttggcgtgg gccaggaaat cacatcctac
     2101 actgcccagg agccagacac atttatggaa cagaaaataa catatcggat ttggagagac
     2161 actgccaact ggctggagat taatccggac actggtgcca tttccactcg ggctgagctg
     2221 gacagggagg attttgagca cgtgaagaac agcacgtaca cagccctaat catagctaca
     2281 gacaatggtt ctccagttgc tactggaaca gggacacttc tgctgatcct gtctgatgtg
     2341 aatgacaacg cccccatacc agaacctcga actatattct tctgtgagag gaatccaaag
     2401 cctcaggtca taaacatcat tgatgcagac cttcctccca atacatctcc cttcacagca
     2461 gaactaacac acggggcgag tgccaactgg accattcagt acaacgaccc aacccaagaa
     2521 tctatcattt tgaagccaaa gatggcctta gaggtgggtg actacaaaat caatctcaag
     2581 ctcatggata accagaataa agaccaagtg accaccttag aggtcagcgt gtgtgactgt
     2641 gaaggggccg ccggcgtctg taggaaggca cagcctgtcg aagcaggatt gcaaattcct
     2701 gccattctgg ggattcttgg aggaattctt gctttgctaa ttctgattct gctgctcttg
     2761 ctgtttcttc ggaggagagc ggtggtcaaa gagcccttac tgcccccaga ggatgacacc
     2821 cgggacaacg tttattacta tgatgaagaa ggaggcggag aagaggacca ggactttgac
     2881 ttgagccagc tgcacagggg cctggacgct cggcctgaag tgactcgtaa cgacgttgca
     2941 ccaaccctca tgagtgtccc ccggtatctt ccccgccctg ccaatcccga tgaaattgga
     3001 aattttattg atgaaaatct gaaagcggct gatactgacc ccacagcccc gccttatgat
     3061 tctctgctcg tgtttgacta tgaaggaagc ggttccgaag ctgctagtct gagctccctg
     3121 aactcctcag agtcagacaa agaccaggac tatgactact tgaacgaatg gggcaatcgc
     3181 ttcaagaagc tggctgacat gtacggaggc ggcgaggacg acggatcctg agaccagacc
     3241 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
     3301 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct
     3361 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     3421 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     3481 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     3541 aactggtgaa actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt
     3601 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     3661 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     3721 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     3781 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     3841 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     3901 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     3961 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     4021 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     4081 gaaagttgga acctcttacg tgcccgatca atcatgacca aaatccctta acgtgagttt
     4141 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4201 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4261 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag c
//
