LOCUS       sequence-248699-        2439 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes mScarlet as a Type 3 part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3_036
FEATURES             Location/Qualifiers
     source          1..2439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     396..415
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1400..1434)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1505..2164)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2079..2098
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(2346..2439,1..495)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
       61 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
      121 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
      181 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
      241 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
      301 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
      361 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
      421 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
      481 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
      541 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtagtcgg
      601 tctcatatgg tatcaaaagg cgaggcggtc attaaagagt tcatgaggtt taaagtccat
      661 atggaaggaa gcatgaatgg tcacgagttt gagatagagg gagagggtga gggaaggccg
      721 tacgaaggga cgcaaactgc gaagttgaag gtaacgaaag gcggccctct gccattctct
      781 tgggacatcc tttcaccgca atttatgtac ggctctaggg ccttcacaaa acaccccgcc
      841 gatattccgg attactataa acaaagtttt cctgagggct tcaagtggga aagagtaatg
      901 aacttcgagg atgggggagc tgtgacggtt actcaagaca ctagcctgga ggacggtact
      961 cttatataca aggtaaaact aagagggacg aattttccgc cagacggccc cgtgatgcaa
     1021 aaaaaaacta tggggtggga agcatccacc gagaggctgt accccgaaga cggggtcctg
     1081 aagggtgata ttaaaatggc attaagatta aaagatggcg ggagatactt agctgacttc
     1141 aagactacat ataaagccaa aaaacctgta cagatgcctg gagcttacaa tgtggatcgt
     1201 aagctggata ttacatccca caacgaagat tataccgtgg tggagcagta cgagcgttca
     1261 gaggggcgtc actccaccgg gggtatggat gaattataca agagcggtct tagaagcagg
     1321 gcgcaagcgt caaattcagc ggtagatgga acagcaggtc caggtagtac tggcagcaga
     1381 ggatcctgag accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
     1441 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat
     1501 caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
     1561 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
     1621 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     1681 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgaa acgaaaaaca
     1741 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     1801 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     1861 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     1921 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa
     1981 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     2041 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     2101 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     2161 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     2221 atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaat catgaccaaa
     2281 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2341 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2401 ctaccagcgg tggtttgttt gccggatcaa gagctacca
//
