LOCUS       sequence-248701-        3933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes cAMPr, a cAMP reporter, as a Type 3 part to be used in the 
            MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3_037
FEATURES             Location/Qualifiers
     source          1..3933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(2298..2332)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2403..3062)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2977..2996
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      3244..3832
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3733..3752
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atgggaaatg cggcagcagc aaagaaaggg tctgaacaag aatctgttaa
       61 ggaatttctc gcgaaggcga aagaggactt tcttaagaag tgggaaacgc caagccaaaa
      121 cactgcacaa ctcgaccaat tcgaccgcat aaagacactg ggaactggaa gcttcggcag
      181 ggttatgctt gttaaacata aagaaagcgg caatcattat gctatgaaaa ttctggataa
      241 acaaaaggta gttaaactca aacagataga acataccctc aacgaaaagc gaatccttca
      301 agcagtgaat ttcccatttc ttgtgaagtt ggagtttagc tttaaagata attccaattt
      361 gtatatggta atggaatacg tcgcaggcgg agaaatgttt agtcatctca gaagaatcgg
      421 gagattttct gaacctcacg cgaggtttta tgctgcacaa attgttttga cattcgaata
      481 cttgcattca cttgatctga tatatcgtga tctcaaaccg gaaaacctgc tgatagatca
      541 acaagggtac atccaagtta ccgattttgg gttcgctaaa agggtcaagg gacggacctg
      601 gacactctgc ggcactccgg aatatctggc tccggaaatc atactcagta agggttataa
      661 taaagccgta gattggtggg cactgggcgt gcttatttat gaaatggcgg cgggctatcc
      721 cccattcttt gccgatcaac cgatacaaat atacgaaaag attgtatcag gaaaagttag
      781 atttccttct cattttagtt ccgatcttaa agatctcctt cgcaatctgc tccaggtcga
      841 cctgactaaa aggttcggca atttgaagaa tggagtgaac gatatcaaga atcataaatg
      901 gttcgctacc accgattgga tcgctattta ccaacgtaaa gtcgaggcgc cttttattcc
      961 taaattcaag ggacccggtg atacttcaaa tttcgatgat tacgaagaag aggaaataag
     1021 ggttagtata aacgaaaagt gcggtaaaga attcaccgaa ttccctggaa atgtgtacat
     1081 aaaagctgat gaacagaaga atgggattaa agcctatttt aagatacggc ataatataga
     1141 aggtggtggg gttcaacttg cgtatcatta tcaacaaaat acacctattg gagatgggcc
     1201 agttcttctt ccagataatc attatctcag tgtccaatct atgctgtcta aggatcctaa
     1261 tgaaaagcgt gaccatatgg tgctcttgga atttgttaca gcagcaggta ttacccttgg
     1321 aatggatgaa ctttataaag gcggaactgg tggctccgag tctatggttt ccaaaggtga
     1381 agaattgttt acaggcgttg ttccgattca agttgaattg gatggagatg tcaatgggca
     1441 taaattttcc gtttcaggag aaggggaagg tgacgcaaca tatggaaaac ttacattgaa
     1501 atttatttgt actacaggga aactcccagt cccgtggcca actctggtaa caactcttac
     1561 atatggggtc caatgtttct ctaggtatcc agatcatatg aaacaacatg acttctttaa
     1621 aagtgctatg cctgagggat atattcaaga acgaacgatt ttctttaaag gtgatgggaa
     1681 ttataaaacc cgagccgaag ttaaatttga aggagataca ctggttaaca ggattgaact
     1741 taaaggaatt gactttaaag aagatgggaa tatactcggc cataaactcg aatataatac
     1801 agctccgcct gcctgtaaag ggcgcaggcg cagaggcgct atttctgcgg aagtgtatac
     1861 agaagaagac gctgctagct acgtccgcaa agtcatccct aaggactaca aaacgatggc
     1921 ggccctggca aaggctatcg agaagaatgt gctcttctcc cacctcgacg acaatgaaag
     1981 gtcagatata ttcgatgcga tgtttcccgt ttcattcatc gccggggaaa cagtgatcca
     2041 acaaggagat gagggagaca acttttatgt catcgatcag ggcgaaatgg atgtttacgt
     2101 gaataacgag tgggcgacat ccgtgggaga gggcggatcc tttggcgaac tggccctcat
     2161 atatggcacc ccgcgggccg caaccgtgaa agctaaaacc aatgtcaagc tctggggtat
     2221 cgatcgtgat tcctatcgca gaattctgat gggttccaca ctcaggaaaa ggaaaatggg
     2281 atcctgagac cagaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca
     2341 gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca
     2401 aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg
     2461 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
     2521 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg
     2581 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgaaac gaaaaacata
     2641 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc
     2701 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
     2761 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
     2821 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg
     2881 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
     2941 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
     3001 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
     3061 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     3121 cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaatca tgaccaaaat
     3181 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3241 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3301 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3361 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3421 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3481 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3541 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3601 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3661 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3721 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3781 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3841 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3901 tgcgttatcc cctgattctg tggataaccg tag
//
