LOCUS       sequence-248707-        3312 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes mouse RelA as a Type 3 part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3_039
FEATURES             Location/Qualifiers
     source          1..3312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      77..665
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     566..585
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1881..2423
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEAALQAQADADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     terminator      complement(2443..2477)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2548..3207)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3122..3141
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
       61 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      721 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtagtcgg tctcatatgg
      781 acgatctgtt tcccctcatc tttccctcag agccagccca ggcttctggg ccttatgtgg
      841 agatcatcga acagccgaag caacggggca tgcgattccg ctataaatgc gaggggcgct
      901 cagcgggcag tattcctggc gagagaagca cagataccac caagacacac cccaccatca
      961 agatcaatgg ctacacagga ccaggaacag ttcgaatctc cctggtcacc aaggatccac
     1021 ctcaccggcc tcatccacat gaacttgtgg ggaaggactg ccgggatggc tactatgagg
     1081 ctgacctctg cccagaccgc agtatccata gcttccagaa cctggggatc cagtgtgtga
     1141 agaagcgaga tctggagcaa gccattagcc agcgaatcca gaccaacaat aacccctttc
     1201 acgttcctat agaggagcag cgcggggact atgacttgaa tgcagtgcgc ctctgcttcc
     1261 aggtgacagt gcgggaccca gcaggcaggc ccctcctcct gacccctgtc ctctcacatc
     1321 cgatttttga taaccgggcc cccaacactg ccgagctcaa gatctgccga gtaaaccgga
     1381 actctgggag ctgcctcggt ggggatgaga tcttcttgct gtgcgacaag gtgcagaaag
     1441 aagacattga ggtgtatttc acgggaccag gctgggaggc acgaggctcc ttttctcaag
     1501 ctgatgtgca tcggcaagtg gccattgtgt tccggactcc tccgtacgcc gaccccagcc
     1561 tccaggctcc tgttcgagtc tccatgcagc tacggcggcc ttctgatcgc gagctcagtg
     1621 agcccatgga gttccagtac ttgccagaca cagatgatcg ccaccggatt gaagaggccg
     1681 cagccaggac ctatgaaacc ttcaagagta tcatgaagaa gagtcctttc aatggaccaa
     1741 ctgaaccccg gcctccaacc cggcgtattg ctgtgcctac ccgaaactca acttctgtcc
     1801 ccaagccagc cccgcagccc tacaccttcc cagcatccct cagcaccatc aactttgatg
     1861 agttttcccc catgctgtta ccatcagggc agatctcaaa ccaggccctg gccttggcac
     1921 cgtcctctgc cccagtcctt gcccagacca tggtcccttc ctcagccatg gtacctctgg
     1981 ctcagccccc agctcctgcc ccagttctaa ccccgggtcc tccccagtcc ctgtctgcac
     2041 ctgttccaaa gagcacccag gctggggaag gcacgctgtc ggaagccgcc ctgcaggccc
     2101 aggcagatgc tgatgaagac ttgggggcct tgcttggcaa cagcacagac ccaggagtgt
     2161 tcacagacct ggcatctgtg gacaactcag agtttcagca gctcctgaac cagggtgtgt
     2221 ccatgtctca ctccacagct gagcccatgc tgatggagta ccctgaagct ataactcgcc
     2281 tggtgacagg gtcccagagg ccccctgacc cagctcccac acccctgggg acctcggggc
     2341 ttcccaatgg actctcagga gatgaagact tctcctccat tgcggacatg gacttctctg
     2401 ctcttttgag tcagatcagc tccggatcct gagaccagac caataaaaaa cgcccggcgg
     2461 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
     2521 aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat cgcagtactg
     2581 ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg
     2641 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac
     2701 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca
     2761 gggattggct gaaacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt
     2821 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg
     2881 gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg
     2941 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg
     3001 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt
     3061 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg
     3121 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
     3181 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa
     3241 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
     3301 gtgcccgatc aa
//
