LOCUS       sequence-248718-        9820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP-tagged FBXL10 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Rona G, Roberti D, Yin Y, Pagan JK, Homer H, Sassani E, Zeke A, 
            Busino L, Rothenberg E, Pagano M
  TITLE     PARP1-dependent recruitment of the FBXL10-RNF68-RNF2 ubiquitin 
            ligase to sites of DNA damage controls H2A.Z loading.
  JOURNAL   Elife. 2018 Jul 9;7. pii: 38771. doi: 10.7554/eLife.38771.
  PUBMED    29985131
REFERENCE   2  (bases 1 to 9820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-07-9"; volume: "7. pii: 38771. doi"; pages: " 
            10.7554/eLife.38771"
COMMENT     Sequence Label: GFP-FBXL10
FEATURES             Location/Qualifiers
     source          1..9820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..734
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             935..1351
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1281..1303
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1319..1335
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1386..1395
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1392..1394,1395..1397,1398..2108)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1392..1394,1395..1397,1398..2108)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1437..1458)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1698..1717)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2045..2066
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6143..6163)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6148..6477
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6328..6463
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6487..7086
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6487..6506)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6983..7003
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7307..7776
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(7906..7923)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8077..8665)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8157..8176)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8825..9616,9617..9685))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9448..9467
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9686..9790)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
       61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      301 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
      361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggcaagctg
      541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      661 gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg
      721 tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct
      781 ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg
      841 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg
      901 tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca ctcccttaag
      961 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt
     1021 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg
     1081 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc
     1141 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc
     1201 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc
     1261 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct
     1321 ttatccagcc ctcactcctt ctctaggcgc cggccggatc ccagtgtggt ggtacgctac
     1381 cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     1441 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     1501 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     1561 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     1621 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     1681 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     1741 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     1801 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     1861 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     1921 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     1981 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     2041 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     2101 tgtacaagtc cggactcaga tctgcgggtc cgcaaatggg gggatctgca gaggatcacc
     2161 ccccacgaaa aagacatgca gcagaaaagc aaaaaaagaa aacagttata tatacaaaat
     2221 gctttgaatt tgagtcggcc acacagcgcc cgattgaccg ccagcgatac gacgagaacg
     2281 aggacttgtc ggacgtggag gagatcgtca gcgtccgcgg cttcagcctg gaggagaagc
     2341 ttcgcagcca gctgtaccag ggggacttcg tgcacgccat ggagggcaaa gatttcaact
     2401 atgagtacgt acagagagaa gctctcaggg ttcccctgat atttcgagaa aaggatggac
     2461 tgggaattaa gatgcctgac cctgatttca cagtccgaga cgtcaaactc ctagtgggga
     2521 gccggcggct tgtggacgtg atggatgtga acacccagaa gggcacggag atgagcatgt
     2581 cccagtttgt gcgttactac gagacgcccg aggcccagcg ggacaagctg tacaacgtca
     2641 tcagcctaga gttcagccac accaagctgg agcacttggt caagcgtccg actgtggtag
     2701 acctggtgga ctgggtggac aacatgtggc cccagcatct gaaggagaag cagacagaag
     2761 ccacgaacgc cattgcagag atgaagtacc cgaaagtgaa aaagtactgt ctgatgagcg
     2821 tgaaaggttg tttcaccgac ttccacatcg actttggagg cacttccgtt tggtaccatg
     2881 ttttccgggg tgggaagatt ttttggctga ttcctccaac gctgcacaat ttggcgctgt
     2941 acgaggagtg ggtgctgtca ggcaaacaga gtgacatctt tctgggagac cgtgtggaac
     3001 gatgccaaag aattgagctg aagcagggct acacattttt catcccttcc ggttggatcc
     3061 atgccgtcta cacccctgta gactctttgg tgttcggcgg aaacatcctg cacagcttta
     3121 acgtgcccat gcagctgcgg atctacgaga tcgaggacag gacgcgggtg cagcccaaat
     3181 tccgttaccc cttctactat gagatgtgct ggtatgtcct ggagagatac gtgtactgtg
     3241 tgacccagcg ctcccacctc actcaggaat accagaggga gtcgatgctt attgatgccc
     3301 cgaggaagcc cagcatagac ggcttctctt cggattcctg gctggagatg gaggaggagg
     3361 cctgtgatca gcagcctcag gaggaggagg agaaggacga ggagggcgag ggcagggaca
     3421 gggcacccaa accgcccacc gatggctcca cttcacccac cagcacgccc tctgaggacc
     3481 aggaggccct cgggaagaag cccaaagcac ctgccctgcg attcctcaaa aggactttgt
     3541 ctaatgagtc ggaggaaagt gtgaagtcca ccacattggc cgtagactac cccaagaccc
     3601 ccaccggctc tcccgccacg gaggtctctg ccaaatggac ccatctcact gagtttgaac
     3661 tgaagggcct gaaagctctg gtggagaaac tggaatccct cccggagaac aagaagtgtg
     3721 tccccgaggg catcgaggac ccccaggcac tcctggaggg tgtgaagaac gtcctgaagg
     3781 agcacgcaga tgatgaccct agtctggcca tcactggggt ccctgtggtg acttggccaa
     3841 agaagactcc aaagaaccgg gctgtgggtc ggcccaaggg gaagctgggc ccggcctccg
     3901 cggtgaagtt ggccgccaac cggacaacgg caggagctcg gcggcgccgg acgcgatgcc
     3961 gcaagtgcga ggcctgcctg cggaccgagt gcggagagtg ccacttctgc aaggacatga
     4021 agaagttcgg gggccccggg cgcatgaagc agagctgcat catgcggcag tgcatcgcgc
     4081 cagtgctgcc ccacaccgcc gtgtgccttg tgtgtggcga ggcggggaag gaagacacgg
     4141 tggaagagga ggaaggcaag tttaacctca tgctcatgga gtgctccatc tgcaatgaaa
     4201 tcatccaccc tggatgcctt aagattaagg agtcagaggg tgtggtcaac gacgagcttc
     4261 caaactgctg ggagtgtccg aagtgtaacc acgccggcaa gaccgggaaa caaaagcgtg
     4321 gccctggctt taagtacgcc tccaacctgc ccggctccct gctcaaggag cagaagatga
     4381 accgggacaa caaggaaggg caggaacctg ccaagcggag gagtgagtgt gaggaggcgc
     4441 cccggcgcag gtcggatgag cactcgaaga aggtgccgcc ggacggcctt ctgcgcagaa
     4501 agtctgacga cgtgcacctg aggaagaagc ggaaatacga gaagccccag gagctgagtg
     4561 gacgcaagcg ggcctcatcg cttcaaacgt cccccggttc ctcctctcac ctctcgccga
     4621 ggccccctct aggcagcagc ctcagcccct ggtggagatc cagtctcact tacttccagc
     4681 agcagctcaa acctggcaaa gaagataagc ttttcaggaa aaagcggcgg tcctggaaga
     4741 acgccgagga ccgcatggcg ctggccaaca agcccctccg gcgcttcaag caggaacccg
     4801 aggacgaact gcccgaggcg ccccccaaga ccagggagag cgaccactcc cgctccagct
     4861 cccccaccgc gggacccagc accgaagggg ccgagggccc ggaggagaag aagaaggtga
     4921 agatgcgccg gaagcggcgg cttcccaaca aggagctgag cagggagctg agcaaggagc
     4981 tcaaccacga gatccagagg acggagaaca gcctggccaa cgagaaccag cagcccatca
     5041 agtcggagcc tgagagcgag ggcgaggagc ccaagcggcc cccgggcatc tgcgagcgtc
     5101 cccaccgctt cagcaagggg ctcaacggca ccccccggga gctgcggcac cagctggggc
     5161 ccagcctgcg cagcccgccc cgtgtcatct cccggccccc accctccgtg tccccgccca
     5221 agtgtatcca gatggagcgc catgtgatcc ggccaccccc catcagcccc ccgcctgact
     5281 cgctacccct ggacgatggg gcagcccacg tcatgcacag ggaggtgtgg atggccgtct
     5341 tcagctacct cagccaccaa gacctgtgtg tgtgcatgcg ggtctgcagg acctggaacc
     5401 gctggtgctg cgataagcgg ttgtggaccc gcattgacct gaaccactgc aagtctatca
     5461 cacccctgat gctgagtggc atcatccggc gacagcccgt ctccctcgac ctcagctgga
     5521 ccaatatctc caagaagcag ctgagctggc tcatcaaccg gctgcctggg ctccgggact
     5581 tggtgctgtc aggctgctca tggatcgcgg tctcggccct ttgcagctcc agttgtccgc
     5641 tgctccggac cctggatgtc cagtgggtgg agggactaaa ggatgcccag atgcgggatc
     5701 tcctgtcccc gcccacagac aacaggccag gtcagatgga caatcggagc aagctccgga
     5761 acatcgtgga gctgcgcctg gcaggcctgg acatcacaga tgcctccctg cggctcatca
     5821 tccgccacat gcccctgctc tccaagctcc acctcagtta ctgtaaccac gtcaccgacc
     5881 agtctatcaa cctgctcact gctgttggca ccaccacccg agactcctta accgagatca
     5941 acctgtctga ctgcaataag gtcactgatc agtgcctgtc cttcttcaaa cgctgtggaa
     6001 acatctgtca tattgacctg aggtactgca agcaagtcac caaggaaggc tgtgagcagt
     6061 tcatagccga gatgtctgtg agtgtccagt ttgggcaagt agaagaaaaa ctcctgcaaa
     6121 aactgagtta gctcgaccct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     6181 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     6241 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     6301 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     6361 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     6421 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     6481 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     6541 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     6601 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     6661 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     6721 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     6781 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     6841 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     6901 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     6961 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     7021 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     7081 gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca
     7141 tcgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc
     7201 tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa
     7261 ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagctga atatgggcca
     7321 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca
     7381 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     7441 agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg
     7501 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag
     7561 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
     7621 cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca
     7681 ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct
     7741 ctgagtgatt gactacccgt cagcgggggt ctttcacatg cagcatgtat caaaattaat
     7801 ttggtttttt ttcttaagta tttacattaa atggccatag ttgcattaat gaatcggcca
     7861 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7921 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7981 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8041 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8101 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8161 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8221 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8281 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8341 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8401 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8461 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     8521 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8581 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8641 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8701 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8761 cacctagatc cttttgcggc cgcaaatcaa tctaaagtat atatgagtaa acttggtctg
     8821 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8881 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8941 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     9001 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     9061 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     9121 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     9181 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     9241 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     9301 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     9361 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     9421 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9481 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9541 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9601 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9661 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9721 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9781 gggttccgcg cacatttccc cgaaaagtgc cacctgcagc
//
