LOCUS       sequence-248719-        1722 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ER Targeting Sequence (N Terminal) as a Type 3a part to be 
            used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3a_006
FEATURES             Location/Qualifiers
     source          1..1722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     135..154
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(422..456)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(527..1186)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1101..1120
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1368..1722,1..234)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
       61 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      121 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      181 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      241 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      301 cctgcgttat cccctgattc tgtggataac cgtagtcggt ctcatatgct gctatccgtg
      361 ccgttgctgc tcggcctcct cggcctggcc gtcgccgtcg agggttcttg agaccagacc
      421 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
      481 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct
      541 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
      601 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
      661 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
      721 aactggtgaa actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt
      781 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
      841 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
      901 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
      961 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     1021 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     1081 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     1141 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     1201 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     1261 gaaagttgga acctcttacg tgcccgatca atcatgacca aaatccctta acgtgagttt
     1321 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1381 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1441 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1501 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1561 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1621 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     1681 ggctgaacgg ggggttcgtg cacacagccc agcttggagc ga
//
