LOCUS       sequence-248722-        9298 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP-tagged FBXL11 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9298)
  AUTHORS   Rona G, Roberti D, Yin Y, Pagan JK, Homer H, Sassani E, Zeke A, 
            Busino L, Rothenberg E, Pagano M
  TITLE     PARP1-dependent recruitment of the FBXL10-RNF68-RNF2 ubiquitin 
            ligase to sites of DNA damage controls H2A.Z loading.
  JOURNAL   Elife. 2018 Jul 9;7. pii: 38771. doi: 10.7554/eLife.38771.
  PUBMED    29985131
REFERENCE   2  (bases 1 to 9298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-07-9"; volume: "7. pii: 38771. doi"; pages: " 
            10.7554/eLife.38771"
COMMENT     Sequence Label: GFP-FBXL11
FEATURES             Location/Qualifiers
     source          1..9298
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..734
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             935..1351
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1281..1303
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1319..1335
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1386..1395
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1392..1394,1395..1397,1398..2108)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1392..1394,1395..1397,1398..2108)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1437..1458)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1698..1717)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2045..2066
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5621..5641)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5626..5955
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5806..5941
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5965..6564
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5965..5984)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6461..6481
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6785..7254
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(7384..7401)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7555..8143)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7635..7654)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8303..9094,9095..9163))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8926..8945
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9164..9268)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
       61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      301 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
      361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggcaagctg
      541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      661 gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg
      721 tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct
      781 ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg
      841 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg
      901 tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca ctcccttaag
      961 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt
     1021 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg
     1081 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc
     1141 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc
     1201 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc
     1261 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct
     1321 ttatccagcc ctcactcctt ctctaggcgc cggccggatc ccagtgtggt ggtacgctac
     1381 cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     1441 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     1501 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     1561 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     1621 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     1681 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     1741 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     1801 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     1861 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     1921 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     1981 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     2041 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     2101 tgtacaagtc cggactcaga tctgaacccg aagaagaaag gattcgttac agccagagat
     2161 tgcgtggtac catgcgacga cgctatgaag atgatggcat ttcagatgat gaaattgaag
     2221 gaaaaagaac ttttgacttg gaagagaaac tgcacaccaa caaatataat gccaattttg
     2281 ttacttttat ggaaggaaaa gattttaatg tagagtatat tcagcggggt ggcttgagag
     2341 atcctctgat tttcaagaat tctgatggac tcggaataaa aatgccggat ccagacttca
     2401 ctgtgaatga tgtcaaaatg tgtgtgggga gtcgtcgcat ggtggatgtc atggacgtga
     2461 acacacagaa aggcattgaa atgaccatgg ctcagtggac acgctactat gagaccccag
     2521 aggaggagcg agagaaactc tataatgtca tcagcctcga gtttagccac accaggctgg
     2581 agaatatggt gcagaggccc tccacggtgg atttcattga ctgggtagac aacatgtggc
     2641 caaggcactt gaaggaaagc cagactgaat caacaaatgc catcttggag atgcagtacc
     2701 ctaaagtgca gaagtactgt ctaatgagtg ttcgaggctg ctatactgac ttccatgtgg
     2761 actttggtgg tacctctgtt tggtatcaca tccatcaagg gggaaaggtc ttctggctca
     2821 tcccccctac agcccacaac ctggagctgt acgagaattg gctgctgtca gggaaacagg
     2881 gagacatctt tctgggtgac cgggtatcag attgtcagcg cattgagctc aagcagggct
     2941 ataccttcgt cattccctca ggctggattc atgctgtgta tactcctaca gacacattag
     3001 tgtttggggg caattttttg catagcttca acatccctat gcagttaaaa atatacaaca
     3061 ttgaagatcg gacacgggtt ccaaataagt ttcgctatcc attctactat gagatgtgtt
     3121 ggtatgtgtt ggagcgctat gtgtactgca taaccaaccg ttcccaccta accaaggaat
     3181 ttcagaaaga gtccctcagc atggatttgg agttaaatgg gttggagtct gggaatgggg
     3241 atgaggaagc agtggatcga gaaccccgac gcttgagcag caggcgttct gtcctcacta
     3301 gccctgtagc gaatggagtc aacctggatt atgatggact gggcaaaacc tgccgaagtc
     3361 ttccaagtct gaagaaaact ttggctgggg actcatcttc tgactgtagc cggggctccc
     3421 acaatggaca agtgtgggat ccccagtgtg ctccccgaaa ggacaggcaa gtgcatctga
     3481 cccattttga gcttgaaggc cttcgctgcc ttgtagataa gttggagtct ctgccactgc
     3541 acaagaaatg tgtccccaca gggatagaag atgaagatgc tctcattgct gatgtaaaga
     3601 ttttgctgga ggagcttgcc aacagcgatc ccaagttagc cctcactgga gttcctatag
     3661 tacagtggcc aaaaagggat aagcttaaat tccccactcg gccaaaggtg cgggttccta
     3721 ccatccccat tacgaagcct cacactatga aaccagctcc acggttaaca cctgtgaggc
     3781 cagctgctgc ctccccgatt gtgtcaggag ccagacggag acgagtgcga tgtcgaaaat
     3841 gcaaagcctg tgtgcaagga gagtgtggtg tttgccacta ctgcagagac atgaagaagt
     3901 ttggggggcc tggacgcatg aagcagtcct gtgtcctccg acagtgcttg gcacccagac
     3961 tgcctcactc agtcacatgt tccctctgtg gagaggtgga tcagaatgaa gagacacaag
     4021 actttgagaa gaaactcatg gaatgctgta tctgcaatga gattgttcat cctggctgcc
     4081 tccagatgga cggagagggg ttgcttaacg aagaattgcc aaattgctgg gaatgtccaa
     4141 agtgctacca ggaggacagc tcggagaaag cccagaagcg gaaaatggaa gagagtgacg
     4201 aagaagctgt gcaagccaaa gtcctgcggc ccctgcggag ctgcgatgag cctctcacgc
     4261 ccccgcctca ttcacccact tccatgctgc agctcatcca tgacccggtt tccccccggg
     4321 gtatggtgac tcggtcatcc cctggggctg gccccagcga ccaccacagt gccagccgcg
     4381 atgagcgctt caaacggcgg cagttgctgc ggctgcaggc cacagagcgc accatggtac
     4441 gggaaaagga gaacaatccc agcggcaaaa aggagctgtc tgaagttgag aaagccaaga
     4501 tccggggatc gtacctcact gtcacgctac agaggcccac caaagagctc cacgggacat
     4561 ccattgtgcc caagctgcag gccatcacgg cctcctctgc caaccttcgc cattcccccc
     4621 gtgtgctagt gcagcactgc ccagcccgaa ccccccagcg tggggatgag gaggggctgg
     4681 ggggagagga ggaggaagag gaggaggagg aggaggaaga tgacagtgca gaggaggggg
     4741 gtgcagccag gctgaatggc cggggcagtt gggctcagga tggagacgaa agctggatgc
     4801 agcgggaggt ctggatgtct gtcttccgct acctcagccg cagagaactt tgtgaatgta
     4861 tgcgagtgtg caagacgtgg tataaatggt gctgcgacaa gagactttgg acaaaaattg
     4921 acttgagtag gtgtaaggcc attgtgcccc aggccctcag tggcatcatc aagaggcagc
     4981 cagtcagcct tgacctcagt tggaccaaca tctctaaaaa gcaactgaca tggctcgtca
     5041 ataggctgcc aggactgaaa gacctcctcc tagcaggctg ctcctggtct gcagtctctg
     5101 ccctcagcac ctccagctgc ccccttctca ggacccttga tcttcggtgg gcagtaggaa
     5161 tcaaggaccc tcaaattcgg gacttgctta ctccaccggc tgataaacca ggtcaggaca
     5221 atcgcagcaa gctccggaac atgaccgact tccggctggc aggccttgac atcacagatg
     5281 ccacgcttcg cctcataatt cgccacatgc ccctcctgtc tcgactcgac ctcagtcact
     5341 gcagccacct tacagatcag tcctccaatc tactcactgc tgtcgggtct tccactcgct
     5401 actctctcac agagctcaat atggcaggtt gcaataaatt gacagaccag accctgatct
     5461 acctacggcg cattgccaac gtcaccttga tcgaccttcg aggatgcaag cagatcactc
     5521 gaaaagcctg cgagcacttc atctcagact tgtccatcaa cagcctctac tgcctgtctg
     5581 acgagaagct gatacagaag atcagctaag tcgaccctgt ggaatgtgtg tcagttaggg
     5641 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5701 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     5761 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     5821 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     5881 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     5941 ctaggctttt gcaaaaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
     6001 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     6061 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     6121 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     6181 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     6241 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     6301 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     6361 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     6421 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     6481 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     6541 atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg
     6601 agcgcacgac cccatgcatc gataaaataa aagattttat ttagtctcca gaaaaagggg
     6661 ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag
     6721 gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg aacagatgga
     6781 acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
     6841 ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc
     6901 tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt
     6961 ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta
     7021 tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct
     7081 caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc
     7141 cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt
     7201 tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcacatgca
     7261 gcatgtatca aaattaattt ggtttttttt cttaagtatt tacattaaat ggccatagtt
     7321 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     7381 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7441 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7501 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7561 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7621 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7681 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7741 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7801 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7861 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7921 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7981 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8041 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8101 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8161 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8221 attatcaaaa aggatcttca cctagatcct tttgcggccg caaatcaatc taaagtatat
     8281 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8341 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8401 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8461 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8521 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8581 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8641 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8701 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8761 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8821 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8881 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8941 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9001 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9061 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9121 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9181 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9241 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgcagc
//
