LOCUS       sequence-24875-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Pitera DJ, Paddon CJ, Newman JD, Keasling JD
  TITLE     Balancing a heterologous mevalonate pathway for improved isoprenoid 
            production in Escherichia coli.
  JOURNAL   Metab Eng. 2007 Mar . 9(2):193-207.
  PUBMED    17239639
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2007 Mar . 9(2):193-207."
COMMENT     Sequence Label: pBAD18HMGR
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(623..634)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tcgcaacagc cagcttgcat gcctgcaggt cgacttagga tttaatgcag gtgacggacc
       61 catctttcaa acgatttata tcagtggcgt ccaaattgtt aggttttgtt ggttcagcag
      121 gtttcctgtt gtgggtcata tgactttgaa ccaaatggcc ggctgctagg gcagcacata
      181 aggataattc acctgccaag acggcacagg caactattct tgctaattga cgtgcgttgg
      241 taccaggagc ggtagcatgc gggcctctta cacctaataa gtccaacatg gcaccttgtg
      301 gttctagaac agtaccacca ccgatggtac ctacttcgat ggatggcatg gatacggaaa
      361 ttctcaaatc accgtccact tctttcatca atgttataca gttggaactt tcaacatttt
      421 gtgcaggatc ttgtcctaat gccaagaaaa cagctgtcac taaattagct gcatgtgcgt
      481 taaatccacc aacagaccca gccattgcag atccaaccaa attcttagca atgttcaact
      541 caaccaatgc ggaaacatca ctttttaaca cttttctgac aacatcacca ggaatagtag
      601 cttctgcgac gacactctta ccacgacctt cgatccagtt gatggcagct ggttttttgt
      661 cggtacagta gttaccagaa ancggagaca acctccatat cttcccagcc atactcttct
      721 accatttgct ttaatgagta tncnacncct tnaaaaaaaa aatttnnccc ttgggccccc
      781 nncantgttc aaatnncngg ananaaaacn cngctaaaaa gtt
//
