LOCUS       sequence-248753-        6741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  4x 1033 b long scaffolds divided by Zn-dependent self-cleaving 
            DNAzyme cassettes, from compatibility group 10, made by method IV, 
            yields linear 1033 b long scaffolds after Zn incubation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6741)
  AUTHORS   Engelhardt FAS, Praetorius F, Wachauf CH, Bruggenthies G, Kohler F, 
            Kick B, Kadletz KL, Pham PN, Behler KL, Gerling T, Dietz H
  TITLE     Custom-Size, Functional, and Durable DNA Origami with 
            Design-Specific Scaffolds.
  JOURNAL   ACS Nano. 2019 Apr 22. doi: 10.1021/acsnano.9b01025.
  PUBMED    30990672
REFERENCE   2  (bases 1 to 6741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Nano. 
            2019 Apr 22. doi: 10.1021/acsnano.9b01025."
COMMENT     Sequence Label: pre_IV_CS10_1033_v1
FEATURES             Location/Qualifiers
     source          1..6741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(4921..5509)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5001..5020)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5680..6471,6472..6540))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6303..6322
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6541..6645)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6713..6731
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cctccccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
       61 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc aagcttgtca
      121 ctccggaagt tagttgagct gtcacagaat gtgacgttga agacttccgg cttaagctct
      181 gaaagggttc tatatctcca ggtagatctg ctcccaatgt aacatgctcg ggacctacaa
      241 ggtgtcagga tcgaagattg cacgacgatg acttacggac agccggacgt actccctgaa
      301 caatgcgatt cgatatacac ggtggtgttc tgtttgggcc ttctgactca aagccaagcc
      361 tggataacag tgtccttcgg gtcctccgtg ttatggagca cggctgctta gagcattgcc
      421 acggagcctt tgaaggcagc gagggcgtgg cctcccgacc gcactagcac aacacaggag
      481 aggaccggcg acatacctgg gtggaagttt catcggaata ctcgtcaaac gactaaccca
      541 tcagccatcg atcgtatgaa tatgtataat ccatccgtac ctagagtcgc gggccagcat
      601 caacgcatgt ggtaaattgg ttggatccgc gagcagtaag aacccttaaa tcaagcttcc
      661 cggcacagcg tcagtggcgg atacgcgcga tgtctgccgt gctgaagtag ggtaagggcc
      721 ctccatgcgg acttcttggt agctccaaga gtggattccc tcgtaagttc ggctacgtca
      781 tttactgcag cctctgcaag cggaggaact ccactatgca cttagggact actgatatca
      841 aattcgccgc cgaaacccgc aagatggtgc tctttaactg gccaatacat agcccagtga
      901 aactgttcgc atatggcgaa cctttctgcg ccacttgttt cgcgcacgtt taggagctta
      961 ttagctaaat gaaccagttc tctggagtga tacagacttg cggcgtcccg taaaccgaga
     1021 gtaacttaac cataaagctg gtgggtatag aacatatagc gatcagtacg gccatttgac
     1081 ggcaaatacc gtctggctgg caagtcgggc tgatgaaatg cctgacagaa cttgaactga
     1141 ccagaagtga gcaaaggcgg tctcaattaa cactttccta tacgacacgt tgaagcgtta
     1201 cctgttaggt aacgtagttg agctgtgtcg tatatagatc cggaagttag ttgagctgtc
     1261 acagaatgtg acgttgaaga cttccggctt aagctctgaa agggttctat atctccaggt
     1321 agatctgctc ccaatgtaac atgctcggga cctacaaggt gtcaggatcg aagattgcac
     1381 gacgatgact tacggacagc cggacgtact ccctgaacaa tgcgattcga tatacacggt
     1441 ggtgttctgt ttgggccttc tgactcaaag ccaagcctgg ataacagtgt ccttcgggtc
     1501 ctccgtgtta tggagcacgg ctgcttagag cattgccacg gagcctttga aggcagcgag
     1561 ggcgtggcct cccgaccgca ctagcacaac acaggagagg accggcgaca tacctgggtg
     1621 gaagtttcat cggaatactc gtcaaacgac taacccatca gccatcgatc gtatgaatat
     1681 gtataatcca tccgtaccta gagtcgcggg ccagcatcaa cgcatgtggt aaattggttg
     1741 gatccgcgag cagtaagaac ccttaaatca agcttcccgg cacagcgtca gtggcggata
     1801 cgcgcgatgt ctgccgtgct gaagtagggt aagggccctc catgcggact tcttggtagc
     1861 tccaagagtg gattccctcg taagttcggc tacgtcattt actgcagcct ctgcaagcgg
     1921 aggaactcca ctatgcactt agggactact gatatcaaat tcgccgccga aacccgcaag
     1981 atggtgctct ttaactggcc aatacatagc ccagtgaaac tgttcgcata tggcgaacct
     2041 ttctgcgcca cttgtttcgc gcacgtttag gagcttatta gctaaatgaa ccagttctct
     2101 ggagtgatac agacttgcgg cgtcccgtaa accgagagta acttaaccat aaagctggtg
     2161 ggtatagaac atatagcgat cagtacggcc atttgacggc aaataccgtc tggctgacaa
     2221 gtcgggctga tgaaatgcct gacagaactt gaactgacca gaagtgagca aaggcggtct
     2281 caattaacac tttcctatac gacacgttga agcgttacct gttaggtaac gtagttgagc
     2341 tgtgtcgtat atacttccgg aagttagttg agctgtcaca gaatgtgacg ttgaagactt
     2401 ccggcttaag ctctgaaagg gttctatatc tccaggtaga tctgctccca atgtaacatg
     2461 ctcgggacct acaaggtgtc aggatcgaag attgcacgac gatgacttac ggacagccgg
     2521 acgtactccc tgaacaatgc gattcgatat acacggtggt gttctgtttg ggccttctga
     2581 ctcaaagcca agcctggata acagtgtcct tcgggtcctc cgtgttatgg agcacggctg
     2641 cttagagcat tgccacggag cctttgaagg cagcgagggc gtggcctccc gaccgcacta
     2701 gcacaacaca ggagaggacc ggcgacatac ctgggtggaa gtttcatcgg aatactcgtc
     2761 aaacgactaa cccatcagcc atcgatcgta tgaatatgta taatccatcc gtacctagag
     2821 tcgcgggcca gcatcaacgc atgtggtaaa ttggttggat ccgcgagcag taagaaccct
     2881 taaatcaagc ttcccggcac agcgtcagtg gcggatacgc gcgatgtctg ccgtgctgaa
     2941 gtagggtaag ggccctccat gcggacttct tggtagctcc aagagtggat tccctcgtaa
     3001 gttcggctac gtcatttact gcagcctctg caagcggagg aactccacta tgcacttagg
     3061 gactactgat atcaaattcg ccgccgaaac ccgcaagatg gtgctcttta actggccaat
     3121 acatagccca gtgaaactgt tcgcatatgg cgaacctttc tgcgccactt gtttcgcgca
     3181 cgtttaggag cttattagct aaatgaacca gttctctgga gtgatacaga cttgcggcgt
     3241 cccgtaaacc gagagtaact taaccataaa gctggtgggt atagaacata tagcgatcag
     3301 tacggccatt tgacggcaaa taccgtctgg ctggcaagtc gggctgatga aatgcctgac
     3361 agaacttgaa ctgaccagaa gtgagcaaag gcggtctcaa ttaacacttt cctatacgac
     3421 acgttgaagc gttacctgtt aggtaacgta gttgagctgt gtcgtatata cgtcggaagt
     3481 tagttgagct gtcacagaat gtgacgttga agacttccgg cttaagctct gaaagggttc
     3541 tatatctcca ggtagatctg ctcccaatgt aacatgctcg ggacctacaa ggtgtcagga
     3601 tcgaagattg cacgacgatg acttacggac agccggacgt actccctgaa caatgcgatt
     3661 cgatatacac ggtggtgttc tgtttgggcc ttctgactca aagccaagcc tggataacag
     3721 tgtccttcgg gtcctccgtg ttatggagca cggctgctta gagcattgcc acggagcctt
     3781 tgaaggcagc gagggcgtgg cctcccgacc gcactagcac aacacaggag aggaccggcg
     3841 acatacctgg gtggaagttt catcggaata ctcgtcaaac gactaaccca tcagccatcg
     3901 atcgtatgaa tatgtataat ccatccgtac ctagagtcgc gggccagcat caacgcatgt
     3961 ggtaaattgg ttggatccgc gagcagtaag aacccttaaa tcaagcttcc cggcacagcg
     4021 tcagtggcgg atacgcgcga tgtctgccgt gctgaagtag ggtaagggcc ctccatgcgg
     4081 acttcttggt agctccaaga gtggattccc tcgtaagttc ggctacgtca tttactgcag
     4141 cctctgcaag cggaggaact ccactatgca cttagggact actgatatca aattcgccgc
     4201 cgaaacccgc aagatggtgc tctttaactg gccaatacat agcccagtga aactgttcgc
     4261 atatggcgaa cctttctgcg ccacttgttt cgcgcacgtt taggagctta ttagctaaat
     4321 gaaccagttc tctggagtga tacagacttg cggcgtcccg taaaccgaga gtaacttaac
     4381 cataaagctg gtgggtatag aacatatagc gatcagtacg gccatttgac ggcaaatacc
     4441 gtctggctgg caagtcgggc tgatgaaatg cctgacagaa cttgaactga ccagaagtga
     4501 gcaaaggcgg tctcaattaa cactttccta tacgacacgt tgaagcgtta cctgttaggt
     4561 aacgtagttg agctgtgtcg tatacattga ccgggagcgc cctgtagcgg cgcattaagc
     4621 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4681 gctcccggga tcggaattcc ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     4741 ggagtccacg ttctttaata gtggactctt gttccaaact ggaaaacgtc tagcggtatc
     4801 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4861 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4921 tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4981 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     5041 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     5101 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     5161 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     5221 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     5281 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     5341 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     5401 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5461 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5521 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5581 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5641 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5701 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5761 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     5821 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5881 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     5941 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     6001 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     6061 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     6121 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     6181 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     6241 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     6301 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     6361 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     6421 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     6481 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     6541 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6601 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6661 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     6721 tatcacgagg cagaatttca g
//
