LOCUS       sequence-248756-        7746 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  7653 b scaffold from compatibility group 3, made by method II, 
            containing 1 Zn-dependent self-cleaving DNAzyme cassette for 
            linearisation into 7560 b scaffold.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Engelhardt FAS, Praetorius F, Wachauf CH, Bruggenthies G, Kohler F, 
            Kick B, Kadletz KL, Pham PN, Behler KL, Gerling T, Dietz H
  TITLE     Custom-Size, Functional, and Durable DNA Origami with 
            Design-Specific Scaffolds.
  JOURNAL   ACS Nano. 2019 Apr 22. doi: 10.1021/acsnano.9b01025.
  PUBMED    30990672
REFERENCE   2  (bases 1 to 7746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Nano. 
            2019 Apr 22. doi: 10.1021/acsnano.9b01025."
COMMENT     Sequence Label: pre_II_CS3_7653_v2
FEATURES             Location/Qualifiers
     source          1..7746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(70..89)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     280..301
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        464..568
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(569..637,638..1429)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(787..806)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1600..2188
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2089..2108
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
       61 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
      121 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
      181 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
      241 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
      301 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
      361 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
      421 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      481 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      541 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      601 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      661 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      721 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      781 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
      841 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      901 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      961 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1021 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1081 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     1141 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1201 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1261 taaatctgga gccggtgagc gtgggtcacg cggtatcatt gcagcactgg ggccagatgg
     1321 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1381 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1441 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1501 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1561 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1621 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1681 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1741 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1801 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1861 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1921 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1981 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2041 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2101 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2161 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2221 ggccttttgc tggccttttg ctcaccttag caagagccgc acgacgacca gaggccagtt
     2281 atccagagtt aggatacctc aatgtgcatc cgctcggttc taaggacatt atttcagtcc
     2341 tttaagatct cccgtataga agcctcacgt tagggggcgc cgtgccttca cgccctccca
     2401 tttaggaata ccttgtctcc gccgtcttta ttcagtagcc ctatgcatta cgatgtggcg
     2461 cttcccccgc gtgggcgcag aaatttactg aggcggattc gaaacgactg tgagggcagg
     2521 ataggtgagc aggcactggc acgtaatcaa ccaacggact cacccgtgtg caggcctaaa
     2581 aacagccctc gaagggcact tggatatgaa tgaacccact tgttttgact cgtggaggcg
     2641 tggttttatt actgtgctca gttaacgccg catgaattta gctctgatca ccgtaagggt
     2701 aactgcacta gacatgttgt gggcatttaa gtcctgcagt atctttttgt taggtggaac
     2761 ggcctagggg taccttccgt gagaaactcc cagatgatgc atgttcgagt acttgtgaaa
     2821 tggatggtcg catcacgttg aagcgttacc tgttaggtaa cgtagttgag ctgtgatgcg
     2881 actttttgag gagggtagtt gagctgtcac agaatgtgac gttgaagccc tcctctcaca
     2941 cattacactg tctcgcgcgg gttgcgtctt gaccggtaca agttgtgtaa ccttacacct
     3001 ctagaaacat tttagcagtc gctccaattg ataccacgac ctcagcgcgc gttgggagac
     3061 cgttgccaaa cctagttttc atgttttcct tcaaacgtgc gatataattc ctcgcaccta
     3121 gccgcctagc tgagaagtgt tatataggtc acttgaaaag agtaggttca gcatatggaa
     3181 cttgggggtt tgttcgctat gcggtaacgc gtgcccctct gacttgtcga tgactcccgg
     3241 cgcggtttca atgcgggcat gtaatcgtcg cgaaccttta ctcaggctat aaagcggcat
     3301 aaccagttgt aactaacaga atacagttct ctctcaacgg caactacaaa atagcatact
     3361 tgagacctac gacagtaggg tcgcgtcaac ctgtgcaaga aaaagctaga agagctgaat
     3421 tctgataatt acagacccat acaccgatta accccgactt ataggcgtaa gtggtgtccc
     3481 ttgtatgacc caggccatat ctatgccccc atataaacca ggtcgacttc ccactgatcg
     3541 aaccaataag gaatgatcgc agcccataaa gtacggttca acgattgctg agcttcagtg
     3601 aggttaacag cggctggtgc ccgtacattg cctcgtcgat tagcctagtg agcgtcgaca
     3661 ggagctttaa ctcctacttt catacgccac cgcacagact tcattgacca cgtatctgag
     3721 actccttgta aactgactac tccagctcca tcaacaatct tgaagaagtt ctatccgatc
     3781 acggttatcg cgagtcttag cgtagccaat gctgggccgt agcgtctact taacggaaag
     3841 aagggtgaaa gctgccacag aaaataaatt actatttaca tttgtaagac aaagttagta
     3901 tggttaaata ctggcttagt ctgcgggaaa cttagccata cgacttggtt tgcctatcgt
     3961 tacagcgtac gacccgttca ccaatcgcat ttgcgatgca gataacaact cggaagctgt
     4021 atgacctctc gagcgttagt cgcagtttta gtagagaaaa cccgatagcc aggcgttcaa
     4081 gcctattccc cattatgcgt ccgctattga cttcgctgga ccctcgcgga gtcggcgaca
     4141 aataacatag aggatatcgt cacgggatag cgctctccat tggaattgta catgagagtt
     4201 ggactgccga acgcatgggt ggagcagcca agcacccggg gactacgaag gtttccgcgc
     4261 atgtgttacc tttcagccga agagaaggtc ggtggatctc gctctttcgg agctcatact
     4321 cctcatgcta caatgttaaa acgcaaaacc tgactcaatc tattgcccgg tcgttggctc
     4381 gcatgcttcc tccttctgcc ccaatccgac atgactgaga tgcgcttgat agcaatgacc
     4441 aagatagtcg tctagattca tctttagcga cgtgacttag atttgcagag gaaacggggt
     4501 gactgtagat tatggcagtt gatatctctc cctgttatgg agtgcggagg ctgagtgcta
     4561 atgttgcccc gcaaagcagt gtcgttagaa gtatatatgc agtggcggtg ttcaatcagg
     4621 atcagcccgt gctgatttct ttgttgggga gaaatgatat tgggcgggct caaacttcaa
     4681 ctccgacggt cgaatgccgg gttcttcgat tcctgtttat tgcgacgcac gctctatttg
     4741 ctggattacc tgcattcacc gaatcgccta atatacgtct aatcacctgt caatttaatc
     4801 ctggtatact atcatccagg gtggtctaaa gtcccatgag ccacgatgat taattttggt
     4861 atgcaccaag gggaggtggc tagcgatccg agccgagctg cgtcaccggt tggaaacacc
     4921 tgcgctaccc tccagtggtc ggaattcgcg tatcagcttg tccgggtcta cgtacacgca
     4981 agacagattt catcataccc cctggggatt cttagagact atatttcgtc cgatgtccac
     5041 caacataagc gaggctacct cttggaggag cattcttgta tccccctcat agaaagaccc
     5101 tgtgacgtct taacaatagg ggcgacctag atccgttttc gtatttgacc ttagtggtaa
     5161 ccgcagtaca acgggcctga agatggtagt cgagtgccaa caccaaagag gctctagccc
     5221 agtgttcggg cgtcgttcta cggtacgtcc atgacggcag cagctaccag attccggctg
     5281 cccatcatgg cattatagtt tatgaagagt gaatcgtagc taattcaagg cagtaacgtt
     5341 catagccgac agctgacgct gggttgttta cccgtaattc ttctccactc tgcttgagcg
     5401 gtgaggaccc cttaatctgc tcgacatcca ctttccatgt aggacgacaa cgcttggaca
     5461 atccaatagt tagatagcta gtcggacttc tggcccgatc taaactcaaa tcgcgggatc
     5521 ttctgtcatt cgtactgatg ccgacccaat ttcaaaaacg taggcgcacc cacaggcgag
     5581 gtacttattt atgtattgct ctgtataagc cttcgcctcg agtcctaagc agcgctttgg
     5641 cttcggtaag gcctcatcac ttcagaaacg ccatccgtcg taagcgtgtg tgcctcccta
     5701 gaatcatagg ccactcgcta cttcgtgggg acaggccgcg aaatgctaga ctcataagaa
     5761 catacggctc ctaatggctg ggacgtcggt tgtcccgaag tggcttgtag gctgtgtgtc
     5821 aagtccagaa ccactccgga cggccagcag acgcggataa tcccggaaat aggtaggatt
     5881 gacgaaccgg atactaggat gccctacggg gcgtagatct ggctccgctt ctgacgagag
     5941 tatcataatt ggccttgtta catcctgtaa gtctaagcta aggcgccagg agatggggta
     6001 ggtgtgctta ctggtacgcc gatacggaca cgagcatcca acagtactat acctaatctc
     6061 ttatattcac atgataacgc acaaactcgt tattgtatag cacgaacaga tatattgcac
     6121 gtgattggac cgaaaatgac tttggacgcc agcttaaaag gcggttgctt caattcgatg
     6181 ggtatctcat ccgaatggac agttggtagg ccccttcagc gattataccg cgacattctc
     6241 tgtcctagca ggtcagggaa ttttccctaa gggctcgtat ccacgcatca atcgatgcgg
     6301 acatggtcag attgcatctg cgtaggaaca gtttcgttgc ggcaaggaac cgcttgcgga
     6361 tcacaccata tttggatccc cgagcaagtc ttcttgggtt agaggcatac gtaagaagca
     6421 cgccgtttgc gcgactttcg agcctaacct ggcagggttt cttaattgtt tgatgtactc
     6481 tatctggaaa ttcgtgatat gccagagcgg cccggccccg gattagtcac acgatctgaa
     6541 cttatcccag gtagtgttta gccgtgtagg gatattcgtt ttgtggttgt gctgcggaag
     6601 ttactacagc aggggacccg aggtccgaac tttaaacttt tgtcagtcgg tcagtatgta
     6661 agcccaccat ggaggtcttc accttgctgc tacgtcatta tcgtgccacg ttgaagcgtt
     6721 acctgttagg taacgtagtt gagctgtggc acgatttttt gaacatccta gttgagctgt
     6781 cacagaatgt gacgttgaag ggatgttccg taactcagaa ctaccgagaa gctattattc
     6841 cgccaacccc tcgtagagtc tgagggatca agagacattt atcgactcag tgcgtaatac
     6901 cgttcgaagc gatagatcaa tggtgctccg tcaggacact atgactagaa caggtatgtc
     6961 aaaccgataa ggcaagcata gtcaacgtgt catgagtgac acttaggtcc tatagtgcga
     7021 cagacacgcc aagaccatag acaatgaggt gacaactaga taagctcgat ccttacttgc
     7081 gcacactccc ccagctaacc aaacatcaga tggacctgta cactagtctt ttacctagga
     7141 gaacctcgtt tatcttatta gcggggagca acaacgagct atagatgcca ctagcgtggc
     7201 cctgcaagtt attcgagatg tcaggcaacc tctgttgcga gagctcgctg caccccactc
     7261 actcctgctt cgtcttccaa ggtcccaagc cgatccatgc tctcaggggt gggtccgtgt
     7321 tgcataaaag tctccaaaac tcttaaggag aggcgcgcac ggatatacat cgcgtttagg
     7381 ttattatgtc ccacggcatg gccaaatgag ttcttgagta gcaaaaggtg cacgagaccc
     7441 cggtgcctaa gattttatac gcttcatcgg gaatcactgc ccgacaattg ctttcgtgtt
     7501 aataatttcg acgtaacggt gctgtctaac atcgagactg caattacccc gccagacctt
     7561 tgcacttcca cactaatttg gtcgatcttt gcttaaccgg gaactatgta gtctatatga
     7621 gaatattgag cataaggtgt cagccagcct ttatccttga ggcagatcag gtctattcgc
     7681 tcagagtaag atgctaacac ccagtagatg acgacgttta attagggccg agagaccaat
     7741 gtcacg
//
