LOCUS       sequence-248769-        4421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express GABAR-alpha-1 N terminal domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Meng X, McGraw CM, Wang W, Jing J, Yeh SY, Wang L, Lopez J, Brown 
            AM, Lin T, Chen W, Xue M, Sillitoe RV, Jiang X, Zoghbi HY
  TITLE     Neurexophilin4 is a selectively expressed alpha-neurexin ligand that
            modulates specific cerebellar synapses and motor functions.
  JOURNAL   Elife. 2019 Sep 16;8. pii: 46773. doi: 10.7554/eLife.46773.
  PUBMED    31524598
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-09-16"; volume: "8. pii: 46773. doi"; pages: " 
            10.7554/eLife.46773"
COMMENT     Sequence Label: pCMV-GABAR-alpha-1N-HA
FEATURES             Location/Qualifiers
     source          1..4421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        44..347
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        348..551
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     502..522
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     548..572
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          689..785
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             1536..1562
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    1577..1711
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1601..1620)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1655..1674
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1822..1838)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1822..1838)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1835..1857)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1846..1862
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1870..1876,1877..1894,1895..1900))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1915..1936
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2053..2070)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2224..2812)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2304..2323)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2983..3774,3775..3843))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3606..3625
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3844..3948)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4016..4034
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4072..4094)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4194..4213
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(4407..4421,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(4421,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgag ttcgagcttg catgcctgca ggtcgttaca taacttacgg
       61 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      121 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      181 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      241 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      301 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      361 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      421 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      481 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      541 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
      601 acctccatag aagacaccgg gaccgatcca gcctccggac tctagaggat ccggtactag
      661 aggaactgaa aaaccagaaa gttaactggt aagtttagtc tttttgtctt ttatttcagg
      721 tcccggatcc ggtggtggtg caaatcaaag aactgctcct cagtggatgt tgcctttact
      781 tctaggcctg tacggaagtg ttacttctgc tctaaaagct gcggaattgt acccgcgggc
      841 ccaggcccga attcatgctc gaggatgaac ttaaagacaa caccactgtc ttcacaagaa
      901 ttttggaccg actgctggac ggttatgaca atcgtctgag accaggtttg ggagagcgtg
      961 taactgaagt gaagaccgac attttcgtca ccagtttcgg accagtttca gaccacgata
     1021 tggagtatac aatagatgtg tttttccgtc aaagttggaa ggatgaaaga ttaaaattca
     1081 aaggacccat gacagtgctc cggctaaaca accttatggc cagtaaaatc tggactccag
     1141 atacattttt ccacaatgga aagaagtctg tggcccacaa catgaccatg cctaataagc
     1201 tcctgcgtat cacagaggat ggcactctgc tgtacaccat gaggttgacc gtgagagctg
     1261 aatgcccaat gcacttggaa gacttcccta tggatgccca tgcctgccca ctaaaattcg
     1321 gaagctatgc ttatacaaga gcagaagttg tctatgagtg gaccagagag ccagcccgtt
     1381 cagtggttgt agcagaagat gggtcacgtt taaaccagta tgaccttctt ggacaaacag
     1441 ttgactctgg aattgttcag tccagtactg gagaatatgt ggttatgacg actcacttcc
     1501 acttgaagag aaaaattggc tctcgaggta ccggctaccc atacgatgtt ccagattacg
     1561 cttaagcggc cgcggggatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     1621 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     1681 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     1741 tcaggttcag ggggaggtgt gggaggtttt ttcggatcct ctagagtcga tctgcaggca
     1801 tgctagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     1861 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     1921 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     1981 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     2041 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2101 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2161 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2221 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2281 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2341 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2401 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2461 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2521 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2581 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2641 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     2701 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2761 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2821 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     2881 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2941 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     3001 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3061 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     3121 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     3181 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     3241 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     3301 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     3361 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     3421 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     3481 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     3541 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     3601 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     3661 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     3721 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     3781 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     3841 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     3901 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     3961 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     4021 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     4081 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     4141 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     4201 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
     4321 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     4381 attaagttgg gtaacgccag ggttttccca gtcacgacgt t
//
