LOCUS       sequence-248772-        4418 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express HA tagged  GABAR-alpha-6 N terminal domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Meng X, McGraw CM, Wang W, Jing J, Yeh SY, Wang L, Lopez J, Brown 
            AM, Lin T, Chen W, Xue M, Sillitoe RV, Jiang X, Zoghbi HY
  TITLE     Neurexophilin4 is a selectively expressed alpha-neurexin ligand that
            modulates specific cerebellar synapses and motor functions.
  JOURNAL   Elife. 2019 Sep 16;8. pii: 46773. doi: 10.7554/eLife.46773.
  PUBMED    31524598
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-09-16"; volume: "8. pii: 46773. doi"; pages: " 
            10.7554/eLife.46773"
COMMENT     Sequence Label: pCMV-GABAR-alpha-6N-HA
FEATURES             Location/Qualifiers
     source          1..4418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        2..305
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        306..509
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     460..480
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     506..530
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          647..743
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             1491..1517
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    1532..1666
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1556..1575)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1610..1629
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1777..1793)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1777..1793)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1790..1812)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1801..1817
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1825..1831,1832..1849,1850..1855))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1870..1891
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2008..2025)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2179..2767)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2259..2278)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2938..3729,3730..3798))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3561..3580
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3799..3903)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3971..3989
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4027..4049)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4149..4168
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4362..4384
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4376..4393
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4377..4393
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
       61 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      121 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      181 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      241 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      301 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
      361 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
      421 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
      481 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac
      541 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccggactc
      601 tagaggatcc ggtactagag gaactgaaaa accagaaagt taactggtaa gtttagtctt
      661 tttgtctttt atttcaggtc ccggatccgg tggtggtgca aatcaaagaa ctgctcctca
      721 gtggatgttg cctttacttc taggcctgta cggaagtgtt acttctgctc taaaagctgc
      781 ggaattgtac ccgcgggccc aggcccgaat tccaacttga agatgaaggc aacttctact
      841 ctgaaaatgt cagtcggatt cttgacaact tgctggaagg ctatgacaac cgtctacggc
      901 caggatttgg gggtgctgta acagaagtca aaacagacat ctatgtgacc agctttggac
      961 ctgtgtcaga tgtggagatg gagtatacaa tggatgtttt cttccgccag acttggactg
     1021 atgagagact gaaatttaaa ggccctgctg agattctgag cttaaataac ttgatggtca
     1081 gtaagatctg gactcctgac acttttttcc ggaatgggaa aaagtcaatt gctcacaaca
     1141 tgactacccc caacaagctc ttccgattaa tgcagaatgg aacaatcctc tataccatga
     1201 ggctcaccat caacgctgat tgccccatga gattggttaa cttccctatg gatggacacg
     1261 cttgtccact caagtttggg agctatgctt atcctaaaac tgaaatcata tatacatgga
     1321 aaaaaggacc actttactca gtagaagtcc cagaagaatc ttctagcctc ctccagtatg
     1381 atttgattgg gcaaacagtt tctagtgaga caattaaatc taacactggt gaatatgtaa
     1441 tcatgacagt ctatttccac ttacaaagga agatgtctcg aggtaccggc tacccatacg
     1501 atgttccaga ttacgcttaa gcggccgcgg ggatccagac atgataagat acattgatga
     1561 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     1621 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     1681 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttcgg atcctctaga
     1741 gtcgatctgc aggcatgcta gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     1801 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     1861 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     1921 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     1981 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     2041 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     2101 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2161 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2221 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2281 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2341 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2401 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2461 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2521 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2581 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     2641 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2701 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2761 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2821 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2881 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2941 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     3001 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     3061 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3121 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3181 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3241 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3301 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3361 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3421 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3481 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3541 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3601 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3661 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3721 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     3781 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     3841 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     3901 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     3961 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     4021 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     4081 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     4141 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     4201 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
     4261 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     4321 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     4381 aacgacggcc agtgagttcg agcttgcatg cctgcagg
//
