LOCUS       sequence-24883-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Lee HH, Kim YS, Kim KH, Heo I, Kim SK, Kim O, Kim HK, Yoon JY, Kim 
            HS, Kim do J, Lee SJ, Yoon HJ, Kim SJ, Lee BG, Song HK, Kim VN, Park
            CM, Suh SW
  TITLE     Structural and functional insights into dom34, a key component of 
            no-go mRNA decay.
  JOURNAL   Mol Cell. 2007 Sep 21. 27(6):938-50.
  PUBMED    17889667
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2007 Sep 21. 27(6):938-50."
COMMENT     Sequence Label: Dom34(265-386)
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             26..48
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             494..511
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgnggaaat tcccnntaca ctaattttgt ttaactttaa gaaggagata taccatgggc
       61 agnggccncc ctttaggnnc ntcacagcag cggcctggtg ccgngcggca gccatatgaa
      121 tccactgtnt gcttcaaagc tgcaagatac tagaaatact ccaaaganat catggtgatg
      181 gatgaatttt tgttgcactt tcaacnagga tgatgataaa ncttggtatg gtgagaaana
      241 ggtanttnan gcggctgagn ntggcncaat tannnattta cngttaacag annaagtttn
      301 gcattcagac tatattgcac naagagagga atattngana ctgatgnnct ccgtatagag
      361 cantggagga nnagcnttgn tattangtac ttngcattcn ctnggagaan aacttgatcn
      421 attgacnggt atagcctgta ttcnaaaata cccgctcccc gatcttgatn aanacgatng
      481 tgaggagctc gagcaccacc accaccacca ctgagatccg gctgctaaca aaancccgaa
      541 aggangctga tttggctgct gcccgccgct gagcaataac tagnanaacc tncntcgggc
      601 ctccaaacgn gtcntggcgc cgtctttngc cgnaaggcgg aactatancc ccgattgcga
      661 acnananccg cctgnnnaan nnnttntgcc cggcggnagg gtgntacncg cacncgacgc
      721 tananttgga caccgcctac gncgnccttt cgnttcttcc ctccntttcg cacgttcgcg
      781 gnttcccgna gnntaaacgg gcncccttag gtccattatg ttnggnctca cccnaaactn
      841 tagngagttc cngtggctcc tgngagttnc cttatgncct ttaang
//
