LOCUS       sequence-248850-         438 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes VSVG tagged GSK3(1-97) for purification by nickel affinity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Smolko CM, Janes KA
  TITLE     An ultrasensitive fiveplex activity assay for cellular kinases.
  JOURNAL   Sci Rep. 2019 Dec 19;9(1):19409. doi: 10.1038/s41598-019-55998-8.
  PUBMED    31857650
REFERENCE   2  (bases 1 to 438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-12-19"; volume: "9(1):19409. doi"; pages: " 
            10.1038/s41598-019-55998-8"
COMMENT     Sequence Label: pET-29b-3xVSVG-GSK3(1-97)
FEATURES             Location/Qualifiers
     source          1..438
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..36
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             37..69
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             70..102
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             391..411
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             418..435
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgtacaccg atatagagat gaacaggctg ggaaagtaca ccgatataga gatgaacagg
       61 ctgggaaagt acaccgatat agagatgaac aggctgggaa agagcggcgg cgggccttcg
      121 ggaggcggcc ctgggggctc gggcagggcg cggactagct cgttcgcgga gcccggcggc
      181 ggaggcggag gaggcggcgg cggccccgga ggctcggcct ccggcccagg cggcaccggc
      241 ggcggaaagg catctgtcgg ggccatgggt gggggcgtcg gggcctcgag ctccgggggt
      301 ggacccggcg gcagcggcgg aggaggcagc ggaggccccg gcgcaggcac tagcttcccg
      361 ccgcccgggg tgaagctggg ccgtgacagc gagaacctat acttccaagg ggtcgagcac
      421 caccaccacc accactga
//
