LOCUS       sequence-248856-        3622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  contains the chaperonin-60 universal target from phytoplasma 
            baceria; used as a control for development of molecular diagnostic 
            assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3622)
  TITLE     Dumonceaux lab phytoplasma plasmids
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ESFY
FEATURES             Location/Qualifiers
     source          1..3622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(715..733)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(716..733)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(751..767)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(751..767)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(764..786)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    775..791
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(799..805,806..823,824..829))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    844..865
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(982..999)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1153..1741)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1233..1252)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1912..2703,2704..2772))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2535..2554
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2773..2877)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2955..3410)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3092..3113)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3304..3323
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3536..3558
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3550..3567
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3551..3567
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3574..3593
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3574..3592
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gccgccatgg cggccgcggg aattcgattg atggggcagg ggatggaaca acgactgcaa
       61 ctcttttagc tcaaaaaatg attcatcgtg gttttaaagc aatagattta ggtgctaatc
      121 ctgttttagt aaaagaagga atagaaaaag caacaaaaca agttatagca aaacttttag
      181 aaaaatcaaa accagtaata acgcaagaag atattgaaaa tgtagcttct atttcatctg
      241 gaagtaaaga aattggaaaa atcattgctt tagcaatgca aaaagtaaca aaaaatggtg
      301 ttattaatgt tgatgaatct aaaggttttg aaaccaaatt agaagtagtt gaaggtatgc
      361 aatatgataa aggttacgct tcaccttatt ttgttaataa cagagaaact atgtcagttg
      421 aattagaaca tcctttaatt ttagtaacag atcataaaat aaataatatt caagaaattt
      481 tacatttatt agaagaagta gttaaacaag gtaaatcatt attaataatt gctgattctg
      541 tagaaaatga agttacaagt attttagtta tcaataaaat gaaaggaaca tttaatatag
      601 ttgttactaa tgcccctggt tttggcgaca accaaatcac tagtgaattc gcggccgcct
      661 gcaggtcgac catatgggag agctcccaac gcgttggatg catagcttga gtattctata
      721 gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
      781 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
      841 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
      901 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
      961 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1021 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1081 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1141 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1201 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1261 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1321 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     1381 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     1441 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     1501 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     1561 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     1621 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1681 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     1741 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     1801 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     1861 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     1921 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1981 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     2041 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     2101 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     2161 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     2221 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     2281 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     2341 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     2401 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     2461 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     2521 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     2581 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     2641 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     2701 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     2761 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     2821 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     2881 atttccccga aaagtgccac ctgatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     2941 aataccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     3001 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     3061 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     3121 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     3181 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     3241 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     3301 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     3361 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca
     3421 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     3481 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     3541 tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg
     3601 ggcccgacgt cgcatgctcc cg
//
