LOCUS       sequence-248858-        3622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  contains the chaperonin-60 universal target from phytoplasma 
            baceria; used as a control for development of molecular diagnostic 
            assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3622)
  TITLE     Dumonceaux lab phytoplasma plasmids
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SCWL
FEATURES             Location/Qualifiers
     source          1..3622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      694..703
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      698..707
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(737..756)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(738..756)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(763..780)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(763..779)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(772..794)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      920..1375
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1007..1026)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1217..1238
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1453..1557
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1558..1626,1627..2418)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1776..1795)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2589..3177
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3078..3097
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3331..3348
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3465..3486
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3501..3506,3507..3524,3525..3531)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3539..3555
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3544..3566
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3563..3579
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3563..3579
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3597..3615
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3597..3614
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 agctatgcat ccaacgcgtt gggagctctc ccatatggtc gacctgcagg cggccgcgaa
       61 ttcactagtg attgatgggt cgggggatgg gacgacgaca gctactgttc tagctcaaaa
      121 tatgattcat aaaggtttca aatttattga tgcaggagca aaatctatct cggttaaaga
      181 aggtatttta aaagcagcaa aaagagtatc tcaagaatta cttaaaaaaa ctaaatatgt
      241 aacaactcaa gaggatattg aaaatgttgc ttctatttct gcaggaaata aagaaatagg
      301 taaaattatc gcttctgcaa tggaaaaagt aactaaaaac ggtgttatta atgttgatga
      361 atccaaagga tttgaaacag aattagaagt agttaaggga atgcaatatg ataagggtta
      421 tgtttctcct cattttgtaa caaataaaga aaatatgtca gtagagttag aaaaagcatt
      481 cgtactagtt actgatcata aaataagtaa tattcatgaa atcagacctt tattagaaga
      541 gatagttaaa aagtctacac ctatattaat gatcgctgat tcatttgaaa atgatgttat
      601 aagtacatta atattcaata aaattagtgg agtatttaac atagtagcga cagatgcccc
      661 tagttttggc gaccatcaaa tcgaattccc gcggccgcca tggcggccgg gagcatgcga
      721 cgtcgggccc aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca
      781 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
      841 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
      901 cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
      961 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     1021 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     1081 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     1141 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     1201 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     1261 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     1321 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttcctga
     1381 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatcagg tggcactttt
     1441 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     1501 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     1561 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     1621 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     1681 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     1741 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     1801 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     1861 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     1921 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     1981 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     2041 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     2101 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     2161 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2221 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     2281 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2341 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     2401 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     2461 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     2521 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     2581 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2641 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     2701 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     2761 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     2821 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     2881 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     2941 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3001 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3061 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3121 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     3181 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     3241 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     3301 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     3361 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     3421 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     3481 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     3541 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctattt
     3601 aggtgacact atagaatact ca
//
