LOCUS       sequence-248882-        2031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express APEX2 fusion to DEAD-box translation factor eIF4A1 in 
            bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Padron A, Iwasaki S, Ingolia NT
  TITLE     Proximity RNA Labeling by APEX-Seq Reveals the Organization of 
            Translation Initiation Complexes and Repressive RNA Granules.
  JOURNAL   Mol Cell. 2019 Aug 22;75(4):875-887.e5. doi: 
            10.1016/j.molcel.2019.07.030.
  PUBMED    31442426
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2019.07"; journalName: "Mol Cell"; date: 
            "2019-08-22- 22"; volume: "75"; issue: "4"; pages: "875-887.e5"
COMMENT     Sequence Label: pHis APEX2-eIF4A1
FEATURES             Location/Qualifiers
     source          1..2031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..750
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="MGKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAG
                     TFDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVA
                     VEVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGH
                     TIGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVD
                     KYAADEDAFFADYAEAHQKLSELGFADA"
     CDS             757..783
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             787..807
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgggaaagt cttacccaac tgtgagtgct gattaccagg acgccgttga gaaggcgaag
       61 aagaagctca gaggcttcat cgctgagaag agatgcgctc ctctaatgct ccgtttggca
      121 ttccactctg ctggaacctt tgacaagggc acgaagaccg gtggaccctt cggaaccatc
      181 aagcaccctg ccgaactggc tcacagcgct aacaacggtc ttgacatcgc tgttaggctt
      241 ttggagccac tcaaggcgga gttccctatt ttgagctacg ccgatttcta ccagttggct
      301 ggcgttgttg ccgttgaggt cacgggtgga cctaaggttc cattccaccc tggaagagag
      361 gacaagcctg agccaccacc agagggtcgc ttgcccgatc ccactaaggg ttctgaccat
      421 ttgagagatg tgtttggcaa agctatgggg cttactgacc aagatatcgt tgctctatct
      481 gggggtcaca ctattggagc tgcacacaag gagcgttctg gatttgaggg tccctggacc
      541 tctaatcctc ttattttcga caactcatac ttcacggagt tgttgagtgg tgagaaggaa
      601 ggtctccttc agctaccttc tgacaaggct cttttgtctg accctgtatt ccgccctctc
      661 gttgacaaat atgcagcgga cgaagatgcc ttctttgctg attacgctga ggctcaccaa
      721 aagctttccg agcttgggtt tgctgatgcc ctgcagctgc ctcccctgga gcgcctgacc
      781 ctggacgaaa acctgtattt tcagggatcc atgtctgcga gccaggattc ccgatccaga
      841 gacaatggcc ccgatgggat ggagcccgaa ggcgtcatcg agagtaactg gaatgagatt
      901 gttgacagct ttgatgacat gaacctctcg gagtcccttc tccgtggcat ctacgcctat
      961 ggttttgaga agccctctgc catccagcag cgagccattc taccttgtat caagggttat
     1021 gatgtgattg ctcaagccca atctgggact gggaaaacgg ccacatttgc catatcgatt
     1081 ctgcagcaga ttgaattaga tctaaaagcc acccaggcct tggtcctagc acccactcga
     1141 gaattggctc agcagataca gaaggtggtc atggcactag gagactacat gggcgcctcc
     1201 tgtcacgcct gtatcggggg caccaacgtg cgtgctgagg tgcagaaact gcagatggaa
     1261 gctccccaca tcatcgtggg tacccctggc cgtgtgtttg atatgcttaa ccggagatac
     1321 ctgtccccca aatacatcaa gatgtttgta ctggatgaag ctgacgaaat gttaagccgt
     1381 ggattcaagg accagatcta tgacatattc caaaagctca acagcaacac ccaggtagtt
     1441 ttgctgtcag ccacaatgcc ttctgatgtg cttgaggtga ccaagaagtt catgagggac
     1501 cccattcgga ttcttgtcaa gaaggaagag ttgaccctgg agggtatccg ccagttctac
     1561 atcaacgtgg aacgagagga gtggaagctg gacacactat gtgacttgta tgaaaccctg
     1621 accatcaccc aggcagtcat cttcatcaac acccggagga aggtggactg gctcaccgag
     1681 aagatgcatg ctcgagattt cactgtatcc gccatgcatg gagatatgga ccaaaaggaa
     1741 cgagacgtga ttatgaggga gtttcgttct ggctctagca gagttttgat taccactgac
     1801 ctgctggcca gaggcattga tgtgcagcag gtttctttag tcatcaacta tgaccttccc
     1861 accaacaggg aaaactatat ccacagaatc ggtcgaggtg gacggtttgg ccgtaaaggt
     1921 gtggctatta acatggtgac agaagaagac aagaggactc ttcgagacat tgagaccttc
     1981 tacaacacct ccattgagga aatgcccctc aatgttgctg acctcatctg a
//
