LOCUS       sequence-248894-       12786 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For generating Tg mice expressing the 2P-activatable RAF system with
            P2A and full-length RAF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12786)
  AUTHORS   Kinjo T, Terai K, Horita S, Nomura N, Sumiyama K, Togashi K, Iwata 
            S, Matsuda M
  TITLE     FRET-assisted photoactivation of flavoproteins for in vivo 
            two-photon optogenetics.
  JOURNAL   Nat Methods. 2019 Sep 9. pii: 10.1038/s41592-019-0541-5. doi: 
            10.1038/s41592-019-0541-5.
  PUBMED    31501546
REFERENCE   2  (bases 1 to 12786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2019 Sep 9. pii: 10.1038/s41592-019-0541-5. doi: 
            10.1038/s41592-019-0541-5."
COMMENT     Sequence Label: pT2ADW_2paRAF
FEATURES             Location/Qualifiers
     source          1..12786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..124
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(125..193,194..985)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(343..362)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1156..1744
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1645..1664
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1898..1915
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2032..2053
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2068..2073,2074..2091,2092..2098)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2106..2122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2111..2133
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2130..2146
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2130..2146
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2165..2185
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2167..2185
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(2242..2253,2254..2441)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     enhancer        3819..4198
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4200..4477
                     /label=chicken beta-actin promoter
     intron          4479..5496
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5420..5442
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5504..5523
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             9658..9714
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9715..10224
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     CDS             10243..10938
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(10376..10394)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     10644..10663
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    11025..11613
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11078..11098)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11496..11507)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    11678..11812
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11702..11721)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11756..11775
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(11934..12096,12097..12108)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(12138..12157)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12139..12157)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12167..12184)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12167..12183)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12176..12198)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12325..12780
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12412..12431)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12622..12643
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
       61 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      121 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      181 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      241 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      301 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      361 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      421 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      481 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
      541 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      601 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
      661 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
      721 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
      781 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
      841 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
      901 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      961 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1021 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1081 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1141 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1201 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1261 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1321 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1381 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1441 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1501 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1561 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1621 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1681 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1741 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     1801 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     1861 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     1921 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     1981 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     2041 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     2101 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     2161 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctcggatctg ctgggcttgc
     2221 tgaaggtagg gggtcaagaa ccagaggtgt aaagtacttg agtaatttta cttgattact
     2281 gtacttaagt attatttttg gggattttta ctttacttga gtacaattaa aaatcaatac
     2341 ttttactttt acttaattac atttttttag aaaaaaaagt actttttact ccttacaatt
     2401 ttatttacag tcaaaaagta cttatttttt ggagatcact tgggccctct agatgcatgc
     2461 cggctggccc aggccatcgg cattccggag cccagggtcc agatttggtt tcagaatgag
     2521 aggtcacgcc agctgaggca gcaccggcgg gaatctcggc cctggcccgg gagacgcggc
     2581 ccgccagaag gccggcgaaa gcggaccgcc gtcaccggat cccagaccgc cctgctcctc
     2641 cgagcctttg agaaggatcg ctttccaggc atcgccgccc gggaggagct ggccagagag
     2701 acgggcctcc cggagtccag gattcagatc tggtttcaga atcgaagggc caggcacccg
     2761 ggacagggtg gcagggcgcc cgcgcaggca ggcggcctgt gcagcgcggc ccccggcggg
     2821 ggtcaccctg ctccctcgtg ggtcgccttc gcccacaccg gcgcgtgggg aacggggctt
     2881 cccgcacccc acgtgccctg cgcgcctggg gctctcccac agggggcttt cgtgagccag
     2941 gcagcgaggg ccgcccccgc gctgcagccc agccaggccg cgccggcaga gggggtctcc
     3001 caacctgccc cggcgcgcgg ggatttcgcc tacgccgccc cggctcctcc ggacggggcg
     3061 ctctcccacc ctcaggctcc tcgctggcct ccgcacccgg gcaaaagccg ggaggaccgg
     3121 gacccgcagc gcgacggcct gccgggcccc tgcgcggtgg cacagcctgg gcccgctcaa
     3181 gcggggccgc agggccaagg ggtgcttgcg ccacccacgt cccaggggag tccgtggtgg
     3241 ggctggggcc ggggtcccca ggtcgccggg gcggcgtggg aaccccaagc cggggcagct
     3301 ccacctcccc agcccgcgcc cccggacgcc tccgcctccg cgcggcaggg gcagatgcaa
     3361 ggcatcccgg cgccctccca ggcgctccag gagccggcgc cctggtctgc actcccctgc
     3421 ggcctgctgc tggatgagct cctggcgagc ccggagtttc tgcagcaggc gcaacctctc
     3481 ctagaaacgg aggccccggg ggagctggag gcctcggaag aggccgcctc gctggaagca
     3541 cccctcagcg aggaagaata ccgggctctg ctggaggagc tttaggacgc ggggttggga
     3601 cggggtcggg tggttcgggg cagggcggtg gcctctcttt cgcggggaac acctggctgg
     3661 ctacggaggg gcgtgtctcc gccccgcccc ctccaccggg ctgaccggcc tgggattcct
     3721 gccttctagg tctaggcccg gtgagagact ccacaccgcg gagaactgcc attctttcct
     3781 gggcatcccg gggatcccag agccggccca gcatgctcga cattgattat tgactagtta
     3841 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     3901 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     3961 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     4021 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     4081 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     4141 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     4201 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
     4261 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg
     4321 gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc
     4381 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg
     4441 gcggcggccc tataaaaagc gaagcgcgcg gcgggcgggg agtcgctgcg acgctgcctt
     4501 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
     4561 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
     4621 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
     4681 ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
     4741 gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt
     4801 tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
     4861 gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg
     4921 ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag
     4981 cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg
     5041 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
     5101 ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc
     5161 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
     5221 atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg
     5281 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
     5341 cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg
     5401 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct
     5461 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
     5521 gtgctgtctc atcattttgg caaagaattc accatgaaga tggacaagaa aaccatcgtg
     5581 tggtttcgga gagatctgag gattgaggac aacccagccc tggccgctgc cgctcacgaa
     5641 ggcagcgtgt ttccagtgtt catctggtgc cccgaggaag agggacagtt ctacccagga
     5701 agggccagcc gctggtggat gaagcagtcc ctggctcacc tgtctcagag tctgaaggcc
     5761 ctggggtcag atctgaccct gatcaaaacc cataacacaa tcagcgccat tctggactgt
     5821 attcgggtga ctggagccac caaagtggtg tttaatcacc tgtatgatcc cgtgagcctg
     5881 gtgcgggacc atacagtgaa ggagaaactg gtggaaagag gcatcagtgt gcagtcatac
     5941 aacggggatc tgctgtatga gccatgggaa atctactgcg agaaggggaa accctttacc
     6001 agcttcaatt cctactggaa gaaatgtctg gacggatctg gaatgagcga gctgattaag
     6061 gagaacatgc acatgaagct gtacatggag ggcaccgtgg acaaccatca cttcaagtgc
     6121 acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtggtc
     6181 gagggcggcc ctctcccctt cgccttcgac atcctggcta ctagcttcct ctacggcagc
     6241 aagaccttca tcaaccacac ccagggcatc cccgacttct tcaagcagtc cttccctgag
     6301 ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac cgctacccag
     6361 gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc
     6421 acatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcatt caccgagacg
     6481 ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca tggccctgaa gctcgtgggc
     6541 gggagccatc tgatcgcaaa cgccaagacc acatatagat ccaagaaacc cgctaagaac
     6601 ctcaagatgc ctggcgtcta ctatgtggac tacagactgg aaagaatcaa ggaggccaac
     6661 aacgagacct acgtcgagca gcacgaggtg gcagtggcca gatacggtag cggtccatgg
     6721 aggctgatgc ctattacagc cgctgccgaa gctatctggg cctgctccat tgaagagctg
     6781 ggactggaaa acgaggccga aaagccttct aatgctctgc tgactcgcgc ctggtcccca
     6841 ggatggtcta acgctgataa actgctgaat gagttcatcg aaaagcagct gattgactat
     6901 gccaaaaact ctaagaaagt ggtgggcaac agcaccagcc tgctgtctcc atacctgcac
     6961 tttggagaga tcagtgtgcg gcacgtgttc cagtgcgcta gaatgaagca gatcatttgg
     7021 gccagggata aaaacagtga gggcgaagag tcagccgacc tgtttctgag gggaatcgga
     7081 ctgagggagt actcccgcta tatttgtttc aattttccct tcactcacga acagtcactg
     7141 ctgagccatc tgcggttctt tccttgggac gctgatgtgg acaagttcaa agcctggcgg
     7201 cagggaagaa caggctatcc cctggtggat gctggaatga gagagctgtg ggccactggc
     7261 tggatgcaca ataggatccg cgtgattgtg agctcctttg ctgtgaagtt cctgctgctg
     7321 ccttggaagt ggggcatgaa atatttttgg gatacactgc tggatgccga cctggagtgt
     7381 gacatcctgg ggtggcagta catctccgga tctattccag atggccatga actggatagg
     7441 ctggacaacc ccgctctgca gggcgctaag tatgaccctg agggagaata catcaggcag
     7501 tggctgcctg agctggctag gctgccaact gaatggattc accatccctg ggacgctcct
     7561 ctgacagtgc tgaaggccag tggggtggag ctgggaacta attacgctaa acctatcgtg
     7621 gatattgaca ccgccagaga gctgctggct aaggccatct cccggaccag agaagcccag
     7681 atcatgattg gcgctgccat ggagcacata cagggagctt ggaagacgat cagcaatggt
     7741 tttggattca aagatgccgt gtttgatggc tccagctgca tctctcctac aatagttcag
     7801 cagtttggct atcagcgccg ggcatcagat gatggcaaac tcacagatcc ttctaagaca
     7861 agcaacacta tccgtgtttt cttgccgaac aagcaaagaa cagtggtcaa tgtgcgaaat
     7921 ggaatgagct tgcatgactg ccttatgaaa gcactcaagg tgaggggcct gcaaccagag
     7981 tgctgtgcag tgttcagact tctccacgaa cacaaaggta aaaaagcacg cttagattgg
     8041 aatactgatg ctgcgtcttt gattggagaa gaacttcaag tagatttcct ggatcatgtt
     8101 cccctcacaa cacacaactt tgctcggaag acgttcctga agcttgcctt ctgtgacatc
     8161 tgtcagaaat tcctgctcaa tggatttcga tgtcagactt gtggctacaa atttcatgag
     8221 cactgtagca ccaaagtacc tactatgtgt gtggactgga gtaacatcag acaactctta
     8281 ttgtttccaa attccactat tggtgatagt ggagtcccag cactaccttc tttgactatg
     8341 cgtcgtatgc gagagtctgt ttccaggatg cctgttagtt ctcagcacag atattctaca
     8401 cctcacgcct tcacctttaa cacctccagt ccctcatctg aaggttccct ctcccagagg
     8461 cagaggtcga catccacacc taatgtccac atggtcagca ccacgctgcc tgtggacagc
     8521 aggatgattg aggatgcaat tcgaagtcac agcgaatcag cctcaccttc agccctgtcc
     8581 agtagcccca acaatctgag cccaacaggc tggtcacagc cgaaaacccc cgtgccagca
     8641 caaagagagc gggcaccagt atctgggacc caggagaaaa acaaaattag gcctcgtgga
     8701 cagagagatt caagctatta ttgggaaata gaagccagtg aagtgatgct gtccactcgg
     8761 attgggtcag gctcttttgg aactgtttat aagggtaaat ggcacggaga tgttgcagta
     8821 aagatcctaa aggttgtcga cccaacccca gagcaattcc aggccttcag gaatgaggtg
     8881 gctgttctgc gcaaaacacg gcatgtgaac attctgcttt tcatggggta catgacaaag
     8941 gacaacctgg caattgtgac ccagtggtgc gagggcagca gcctctacaa acacctgcat
     9001 gtccaggaga ccaagtttca gatgttccag ctaattgaca ttgcccggca gacggctcag
     9061 ggaatggact atttgcatgc aaagaacatc atccatagag acatgaaatc caacaatata
     9121 tttctccatg aaggcttaac agtgaaaatt ggagattttg gtttggcaac agtaaagtca
     9181 cgctggagtg gttctcagca ggttgaacaa cctactggct ctgtcctctg gatggcccca
     9241 gaggtgatcc gaatgcagga taacaaccca ttcagtttcc agtcggatgt ctactcctat
     9301 ggcatcgtat tgtatgaact gatgacgggg gagcttcctt attctcacat caacaaccga
     9361 gatcagatca tcttcatggt gggccgagga tatgcctccc cagatcttag taagctatat
     9421 aagaactgcc ccaaagcaat gaagaggctg gtagctgact gtgtgaagaa agtaaaggaa
     9481 gagaggcctc tttttcccca gatcctgtct tccattgagc tgctccaaca ctctctaccg
     9541 aagatcaacc ggagcgcttc cgagccatcc ttgcatcggg cagcccacac tgaggatatc
     9601 aatgcttgca cgctgaccac gtccccgagg ctgcctgtct tcctcgaggg aagcggagcg
     9661 acgaatttta gtctactgaa acaagcggga gacgtggagg aaaaccctgg acctatgaat
     9721 ggagctatag gaggtgacct tttgctcaat tttcctgaca tgtcggtcct agagcgccaa
     9781 agggctcacc tcaagtacct caatcccacc tttgattctc ctctcgccgg cttctttgcc
     9841 gattcttcaa tgattaccgg cggcgagatg gacagctatc tttcgactgc cggtttgaat
     9901 cttccgatga tgtacggtga gacgacggtg gaaggtgatt caagactctc aatttcgccg
     9961 gaaacgacgc ttgggactgg aaatttcaag gcagcgaagt ttgatacaga gactaaggat
    10021 tgtaatgagg cggcgaagaa gatgacgatg aacagagatg acctagtaga agaaggagaa
    10081 gaagagaagt cgaaaataac agagcaaaac aatgggagca caaaaagcat caagaagatg
    10141 aaacacaaag ccaagaaaga agagaacaat ttctctaatg attcatctaa agtgacgaag
    10201 gaattggaga aaacggatta tattcatctc gagggatctg gaatggtgag caagggcgag
    10261 gcagtgatca aggagttcat gcggttcaag gtgcacatgg agggctccat gaacggccac
    10321 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
    10381 ctgaaggtga ccaagggtgg ccccctgccc ttctcctggg acatcctgtc ccctcagttc
    10441 atgtacggct ccagggcctt catcaagcac cccgccgaca tccccgacta ctataagcag
    10501 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgccgtg
    10561 accgtgaccc aggacacctc cctggaggac ggcaccctga tctacaaggt gaagctccgc
    10621 ggcaccaact tccctcctga cggccccgta atgcagaaga agacaatggg ctgggaagcg
    10681 tccaccgagc ggttgtaccc cgaggacggc gtgctgaagg gcgacattaa gatggccctg
    10741 cgcctgaagg acggaggccg ctacctggcg gacttcaaga ccacctacaa ggccaagaag
    10801 cccgtgcaga tgcccggcgc ctacaacgtc gaccgcaagt tggacatcac ctcccacaac
    10861 gaggactaca ccgtggtgga acagtacgaa cgctccgagg gccgccactc caccggcggc
    10921 atggacgagc tgtacaagtc tagaaagatg agcaaagatg gtaaaaagaa gaaaaagaag
    10981 tcaaagacaa agtgtgtaat tatgtaagtc gacggatcct cgataatcaa cctctggatt
    11041 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
    11101 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
    11161 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
    11221 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
    11281 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
    11341 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
    11401 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
    11461 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
    11521 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
    11581 cgagtcggat ctccctttgg gccgcctccc cgcatctcga caaaggatcc aatggaggcc
    11641 gccaccgcgg tggagctcga attaattcat cgatgatgat ccagacatga taagatacat
    11701 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
    11761 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
    11821 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
    11881 gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctagatcaga tctttcttgc
    11941 ttttactttt acttccttaa tactcaagta caattttaat ggagtacttt tttactttta
    12001 ctcaagtaag attctagcca gatactttta cttttaattg agtaaaattt tccctaagta
    12061 cttgtacttt cacttgagta aaatttttga gtacttttta cacctctgtc aagaaccata
    12121 tgccggtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
    12181 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
    12241 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
    12301 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
    12361 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
    12421 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
    12481 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
    12541 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
    12601 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
    12661 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
    12721 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
    12781 aggtgg
//
