LOCUS       sequence-248900-        3621 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  contains the chaperonin-60 universal target from phytoplasma 
            baceria; used as a control for development of molecular diagnostic 
            assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3621)
  TITLE     Dumonceaux lab phytoplasma plasmids
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CVB
FEATURES             Location/Qualifiers
     source          1..3621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1..17
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      753..762
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      757..766
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(796..815)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(797..815)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(822..839)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(822..838)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(831..853)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      979..1434
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1066..1085)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1276..1297
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1512..1616
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1617..1685,1686..2477)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1835..1854)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2648..3236
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3137..3156
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3390..3407
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3524..3545
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3560..3565,3566..3583,3584..3590)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3598..3614
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(3603..3621,1..4)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agctatttag gtgacactat agaatactca
       61 agctatgcat ccaacgcgtt gggagctctc ccatatggtc gacctgcagg cggccgcgaa
      121 ttcactagtg attgatgggg caggggatgg aaccacgaca gcaactgttt tggcacaaag
      181 tatgattcat cgtggttttg atgcaattga tgcaggagct aatcctgttt tagtaaaaga
      241 aggaattgag ttagcttcat taacaattgc caaaaaactt ttatctaaat ctaaaaaagt
      301 agacgcccaa gaagatattc aaaatgtggc ttctgtttca tcaggtagta aagaaattgg
      361 aaaaatcatt gcccaagcga tgcaaaaagt aggtaaagat ggagttatta atgttgatga
      421 atccaaaggt tttgaaacag aattagaagt tgttgaagga ttgcagtacg ataaaggata
      481 tgcttctcct tattttgtct ctgatagaga aagcatgaca gtacatttag aaaatgcttt
      541 agttttagta actgatcata aaattagtac tgtgcaagaa attgtaccta ttttggaaga
      601 agtagtaaaa gcatctagac ctttgttaat tgtagctgaa tcagtggaaa atgaagtttt
      661 aggtgttttg gtagctaata aattaagagg aacttttaat gtagttgtaa ctaatgcccc
      721 tggttttggc gacaaccaat cgaattcccg cggccgccat ggcggccggg agcatgcgac
      781 gtcgggccca attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa
      841 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
      901 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
      961 agcctgaatg gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     1021 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     1081 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     1141 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     1201 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     1261 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     1321 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     1381 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttcctgat
     1441 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatcaggt ggcacttttc
     1501 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     1561 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     1621 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     1681 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     1741 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     1801 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     1861 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     1921 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     1981 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     2041 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     2101 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     2161 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     2221 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     2281 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     2341 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     2401 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     2461 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     2521 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     2581 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2641 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2701 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2761 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     2821 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2881 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2941 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3001 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3061 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3121 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3181 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3241 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3301 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     3361 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     3421 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     3481 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     3541 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     3601 tgagcggata acaatttcac a
//
