LOCUS       sequence-248916-       14647 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Over-expression of human BLM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14647)
  AUTHORS   Gratia M, Rodero MP, Conrad C, Bou Samra E, Maurin M, Rice GI, Duffy
            D, Revy P, Petit F, Dale RC, Crow YJ, Amor-Gueret M, Manel N
  TITLE     Bloom syndrome protein restrains innate immune sensing of 
            micronuclei by cGAS.
  JOURNAL   J Exp Med. 2019 May 6;216(5):1199-1213. doi: 10.1084/jem.20181329. 
            Epub 2019 Apr 1.
  PUBMED    30936263
REFERENCE   2  (bases 1 to 14647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1084/jem.20181329"; journalName: "J Exp Med"; date: "2019-05-6- 
            6"; volume: "216"; issue: "5"; pages: "1199-1213"
COMMENT     Sequence Label: pTRIP-CMV-puro-2A-BLM
FEATURES             Location/Qualifiers
     source          1..14647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3254..3557
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3558..3761
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3712..3732
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3801..4397
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3801..3820)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4297..4317
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4407..4463
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             8998..9178
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10706..10725)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(10939..10958)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11058..11080
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11118..11136)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11204..11308
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11309..11377,11378..12169)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11527..11546)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12340..12928
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12829..12848
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13082..13099
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(13169..13189)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        13174..13503
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      13354..13489
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 atttttaagt gtaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtatagtaat caattacggg gtcattagtt catagcccat
     3241 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     3301 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     3361 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     3421 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     3481 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     3541 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     3601 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     3661 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     3721 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga
     3781 tcccgcgggc tagcgccacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     3841 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     3901 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     3961 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     4021 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     4081 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     4141 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     4201 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     4261 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     4321 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     4381 cccgcaagcc cggtgccgga agcggagcta ctaacttcag cctgctgaag caggctggag
     4441 acgtggagga gaaccctgga cctggatcca ctagtatggc tgctgttcct caaaataatc
     4501 tacaggagca actagaacgt cactcagcca gaacacttaa taataaatta agtctttcaa
     4561 aaccaaaatt ttcaggtttc acttttaaaa agaaaacatc ttcagataac aatgtatctg
     4621 taactaatgt gtcagtagca aaaacacctg tattaagaaa taaagatgtt aatgttaccg
     4681 aagacttttc cttcagtgaa cctctaccca acaccacaaa tcagcaaagg gtcaaggact
     4741 tctttaaaaa tgctccagca ggacaggaaa cacagagagg tggatcaaaa tcattattgc
     4801 cagatttctt gcagactccg aaggaagttg tatgcactac ccaaaacaca ccaactgtaa
     4861 agaaatcccg ggatactgct ctcaagaaat tagaatttag ttcttcacca gattctttaa
     4921 gtaccatcaa tgattgggat gatatggatg actttgatac ttctgagact tcaaaatcat
     4981 ttgttacacc accccaaagt cactttgtaa gagtaagcac tgctcagaaa tcaaaaaagg
     5041 gtaagagaaa cttttttaaa gcacagcttt atacaacaaa cacagtaaag actgatttgc
     5101 ctccaccctc ctctgaaagc gagcaaatag atttgactga ggaacagaag gatgactcag
     5161 aatggttaag cagcgatgtg atttgcatcg atgatggccc cattgctgaa gtgcatataa
     5221 atgaagatgc tcaggaaagt gactctctga aaactcattt ggaagatgaa agagataata
     5281 gcgaaaagaa gaagaatttg gaagaagctg aattacattc aactgagaaa gttccatgta
     5341 ttgaatttga tgatgatgat tatgatacgg attttgttcc accttctcca gaagaaatta
     5401 tttctgcttc ttcttcctct tcaaaatgcc ttagtacgtt aaaggacctt gacacatctg
     5461 acagaaaaga ggatgttctt agcacatcaa aagatctttt gtcaaaacct gagaaaatga
     5521 gtatgcagga gctgaatcca gaaaccagca cagactgtga cgctagacag ataagtttac
     5581 agcagcagct tattcatgtg atggagcaca tctgtaaatt aattgatact attcctgatg
     5641 ataaactgaa acttttggat tgtgggaacg aactgcttca gcagcggaac ataagaagga
     5701 aacttctaac ggaagtagat tttaataaaa gtgatgccag tcttcttggc tcattgtgga
     5761 gatacaggcc tgattcactt gatggcccta tggagggtga ttcctgccct acagggaatt
     5821 ctatgaagga gttaaatttt tcacaccttc cctcaaattc tgtttctcct ggggactgtt
     5881 tactgactac caccctagga aagacaggat tctctgccac caggaagaat ctttttgaaa
     5941 ggcctttatt caatacccat ttacagaagt cctttgtaag tagcaactgg gctgaaacac
     6001 caagactagg aaaaaaaaat gaaagctctt atttcccagg aaatgttctc acaagcactg
     6061 ctgtgaaaga tcagaataaa catactgctt caataaatga cttagaaaga gaaacccaac
     6121 cttcctatga tattgataat tttgacatag atgactttga tgatgatgat gactgggaag
     6181 acataatgca taatttagca gccagcaaat cttccacagc tgcctatcaa cccatcaagg
     6241 aaggtcggcc aattaaatca gtatcagaaa gactttcctc agccaagaca gactgtcttc
     6301 cagtgtcatc tactgctcaa aatataaact tctcagagtc aattcagaat tatactgaca
     6361 agtcagcaca aaatttagca tccagaaatc tgaaacatga gcgtttccaa agtcttagtt
     6421 ttcctcatac aaaggaaatg atgaagattt ttcataaaaa atttggcctg cataatttta
     6481 gaactaatca gctagaggcg atcaatgctg cactgcttgg tgaagactgt tttatcctga
     6541 tgccgactgg aggtggtaag agtttgtgtt accagctccc tgcctgtgtt tctcctgggg
     6601 tcactgttgt catttctccc ttgagatcac ttatcgtaga tcaagtccaa aagctgactt
     6661 ccttggatat tccagctaca tatctgacag gtgataagac tgactcagaa gctacaaata
     6721 tttacctcca gttatcaaaa aaagacccaa tcataaaact tctatatgtc actccagaaa
     6781 agatctgtgc aagtaacaga ctcatttcta ctctggagaa tctctatgag aggaagctct
     6841 tggcacgttt tgttattgat gaagcacatt gtgtcagtca gtggggacat gattttcgtc
     6901 aagattacaa aagaatgaat atgcttcgcc agaagtttcc ttctgttccg gtgatggctc
     6961 ttacggccac agctaatccc agggtacaga aggacatcct gactcagctg aagattctca
     7021 gacctcaggt gtttagcatg agctttaaca gacataatct gaaatactat gtattaccga
     7081 aaaagcctaa aaaggtggca tttgattgcc tagaatggat cagaaagcac cacccatatg
     7141 attcagggat aatttactgc ctctccaggc gagaatgtga caccatggct gacacgttac
     7201 agagagatgg gctcgctgct cttgcttacc atgctggcct cagtgattct gccagagatg
     7261 aagtgcagca gaagtggatt aatcaggatg gctgtcaggt tatctgtgct acaattgcat
     7321 ttggaatggg gattgacaaa ccggacgtgc gatttgtgat tcatgcatct ctccctaaat
     7381 ctgtggaggg ttactaccaa gaatctggca gagctggaag agatggggaa atatctcact
     7441 gcctgctttt ctatacctat catgatgtga ccagactgaa aagacttata atgatggaaa
     7501 aagatggaaa ccatcataca agagaaactc acttcaataa tttgtatagc atggtacatt
     7561 actgtgaaaa tataacggaa tgcaggagaa tacagctttt ggcctacttt ggtgaaaatg
     7621 gatttaatcc tgatttttgt aagaaacacc cagatgtttc ttgtgataat tgctgtaaaa
     7681 caaaggatta taaaacaaga gatgtgactg acgatgtgaa aagtattgta agatttgttc
     7741 aagaacatag ttcatcacaa ggaatgagaa atataaaaca tgtaggtcct tctggaagat
     7801 ttactatgaa tatgctggtc gacattttct tggggagtaa gagtgcaaaa atccagtcag
     7861 gtatatttgg aaaaggatct gcttattcac gacacaatgc cgaaagactt tttaaaaagc
     7921 tgatacttga caagattttg gatgaagact tatatatcaa tgccaatgac caggcgatcg
     7981 cttatgtgat gctcggaaat aaagcccaaa ctgtactaaa tggcaattta aaggtagact
     8041 ttatggaaac agaaaattcc agcagtgtga aaaaacaaaa agcgttagta gcaaaagtgt
     8101 ctcagaggga agagatggtt aaaaaatgtc ttggagaact tacagaagtc tgcaaatctc
     8161 tggggaaagt ttttggtgtc cattacttca atatttttaa taccgtcact ctcaagaagc
     8221 ttgcagaatc tttatcttct gatcctgagg ttttgcttca aattgatggt gttactgaag
     8281 acaaactgga aaaatatggt gcggaagtga tttcagtatt acagaaatac tctgaatgga
     8341 catcgccagc tgaagacagt tccccaggga taagcctgtc cagcagcaga ggccccggaa
     8401 gaagtgccgc tgaggagctc gacgaggaaa tacctgtatc ttcccactac tttgcaagta
     8461 aaaccagaaa tgaaaggaag aggaaaaaga tgccagcctc ccaaaggtct aagaggagaa
     8521 aaactgcttc cagtggttcc aaggcaaagg gggggtctgc cacatgtaga aagatatctt
     8581 ccaaaacgaa atcctccagc atcattggat ccagttcagc ctcacatact tctcaagcga
     8641 catcaggagc caatagcaaa ttggggatta tggctccacc gaagcctata aatagaccgt
     8701 ttcttaagcc ttcatatgca ttctcataac tcgagaccta gaaaaacatg gagcaatcac
     8761 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     8821 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     8881 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     8941 acgaagacaa aatcgtcgag agatgctgca tataagcagc tgctttttgc ttgtactggg
     9001 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     9061 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     9121 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     9181 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     9241 agtgagaggc cttgacatta taatagattt agcaggaatt gaactaggag tggagcacac
     9301 aggcaaagct gcagaagtac ttggaagaag ccaccagaga tactcacgat tctgcacata
     9361 cctggctaat cccagatcct aaggattaca ttaagtttac taacatttat ataatgattt
     9421 atagtttaaa gtataaactt atctaattta ctattctgac agatattaat taatcctcaa
     9481 atatcataag agatgattac tattatcccc atttaacaca agaggaaact gagagggaaa
     9541 gatgttgaag taattttccc acaattacag catccgttag ttacgactct atgatcttct
     9601 gacacaaatt ccatttactc ctcaccctat gactcagtcg aatatatcaa agttatggac
     9661 attatgctaa gtaacaaatt acccttttat atagtaaata ctgagtagat tgagagaaga
     9721 aattgtttgc aaacctgaat agcttcaaga agaagagaag tgaggataag aataacagtt
     9781 gtcatttaac aagttttaac aagtaacttg gttagaaagg gattcaaatg cataaagcaa
     9841 gggataaatt tttctggcaa caagactata caatataacc ttaaatatga cttcaaataa
     9901 ttgttggaac ttgataaaac taattaaata ttattgaaga ttatcaatat tataaatgta
     9961 atttactttt aaaaagggaa catagaaatg tgtatcatta gagtagaaaa caatccttat
    10021 tatcacaatt tgtcaaaaca agtttgttat taacacaagt agaatactgc attcaattaa
    10081 gttgactgca gattttgtgt tttgttaaaa ttagaaagag ataacaacaa tttgaattat
    10141 tgaaagtaac atgtaaatag ttctacatac gttcttttga catcttgttc aatcattgat
    10201 cgaagttctt tatcttggaa gaatttgttc caaagactct gaaataagga aaacaatcta
    10261 ttatatagtc tcacaccttt gttttacttt tagtgatttc aatttaataa tgtaaatggt
    10321 taaaatttat tcttctctga gatcatttca cattgcagat agaaaacctg agactggggt
    10381 aatttttatt aaaatctaat ttaatctcag aaacacatct ttattctaac atcaattttt
    10441 ccagtttgat attatcatat aaagtcagcc ttcctcatct gcaggttcca caacaaaaat
    10501 ccaaccaact gtggatcaaa aatattggga aaaaattaaa aatagcaata caacaataaa
    10561 aaaatacaaa tcagaaaaac agcacagtat aacaacttta tttagcattt acaatctatt
    10621 aggtattata agtaatctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc
    10681 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
    10741 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
    10801 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc
    10861 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
    10921 cgaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
    10981 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
    11041 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
    11101 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
    11161 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
    11221 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
    11281 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
    11341 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
    11401 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
    11461 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
    11521 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
    11581 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
    11641 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
    11701 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
    11761 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
    11821 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
    11881 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
    11941 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
    12001 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
    12061 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
    12121 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
    12181 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    12241 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    12301 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
    12361 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
    12421 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
    12481 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
    12541 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
    12601 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
    12661 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    12721 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
    12781 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    12841 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    12901 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
    12961 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
    13021 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
    13081 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
    13141 gcgttggccg attcattaat gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
    13201 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
    13261 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
    13321 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
    13381 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
    13441 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
    13501 aaaaagcttg gacacaagac aggcttgcga gatatgtttg agaataccac tttatcccgc
    13561 gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg tttttagtgc
    13621 gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt aagccgtaga
    13681 taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg acatgttgca
    13741 gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga aaggcattat
    13801 tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt
    13861 catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg
    13921 ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc
    13981 ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc
    14041 gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc gcaagatacc
    14101 tggattgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa
    14161 tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg ggttacaata
    14221 gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct
    14281 gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg
    14341 cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact ggtatcgcat
    14401 gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca
    14461 ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg atacggtgat
    14521 tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg aaggaacctt
    14581 acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa
    14641 atataaa
//
