LOCUS       sequence-248918-         561 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A repair construct to express GFP-ARF16-PB1-MCS and OsTIR1 under the
            control of the CMV promoter from the human AAVS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Sathyan KM, McKenna BD, Anderson WD, Duarte FM, Core L, Guertin MJ
  TITLE     An improved auxin-inducible degron system preserves native protein 
            levels and enables rapid and specific protein depletion.
  JOURNAL   Genes Dev. 2019 Oct 1;33(19-20):1441-1455. doi: 
            10.1101/gad.328237.119. Epub 2019 Aug 29.
  PUBMED    31467088
REFERENCE   2  (bases 1 to 561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2019-10-1"; volume: "33(19-20):1441-1455. doi"; pages:
            " 10.1101/gad.328237.119. Epub 2019 Aug 29"
COMMENT     Sequence Label: pMGS55 (GFP-ARF16-PB1-MCS-P2A-OsTIR1)
FEATURES             Location/Qualifiers
     source          1..561
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..24
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atgcctaaga agaagcggaa ggtgggcagc acactgactt ccgggcagcg ggatcaccgg
       61 gacgtcgaga acgagctgag ctccgccgcc ttcagctccc agagcttcgg ggtgcccgac
      121 atgagcttta agcccggctg cagcagcgac gtggccgtga ccgacgccgg gatgcccagc
      181 caggggctgt ggaacaacca gacccagcgg atgaggacat tcacaaaggt gcagaagcgg
      241 gggagcgtgg gccggagcat cgatatcacc aggtacaggg attacgatga gctgaggcat
      301 gatctcgcct gtatgttcgg catccagggc cagctggagg acccctaccg gatggactgg
      361 aagctggtgt acgtggacca cgagaacgat atcctgctgg tgggcgatga cccctgggag
      421 gagttcgtgg gctgcgtgaa gagcattaag attctgtctg ctgctgaggt gcagcagatg
      481 agcctggatg gagatctggg aggagtgcca cctcagactc aggcctgtag tgctagtgat
      541 gatgctaacg cctggagagg g
//
