LOCUS       sequence-248922-        2507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tuner sRNA booster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Bartoli V, Meaker GA, di Bernardo M, Gorochowski TE
  TITLE     Tunable genetic devices through simultaneous control of 
            transcription and translation.
  JOURNAL   Nat Commun. 2020 Apr 29;11(1):2095. doi: 10.1038/s41467-020-15653-7.
  PUBMED    32350250
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-04-29"; volume: "11(1):2095. doi"; pages: " 
            10.1038/s41467-020-15653-7"
COMMENT     Sequence Label: pVB005
FEATURES             Location/Qualifiers
     source          1..2507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        386..404
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      471..518
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(650..667)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(760..1348)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(840..859)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1519..2310,2311..2379))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2142..2161
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2380..2484)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggccacgtg tcttgtccag agctctaata cgactcacta taggggggtc atgactggga
      421 cacgccagtc atgagaatac agacataaag caagccagag attaacgaag ctagcataac
      481 cccttggggc ctctaaacgg gtcttgaggg gttttttggg tacctggagc acaagactgg
      541 cctcatgggc cttccgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      601 attaacatgg tcatagctgt ttccttgcgt attgggcgct ctccgcttcc tcgctcactg
      661 actcgctgcg ctcggtcgtt cgggtaaagc ctggggtgcc taatgagcaa aaggccagca
      721 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
      781 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      841 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
      901 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
      961 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1021 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1081 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1141 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1201 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1261 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1321 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1381 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1441 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1501 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     1561 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     1621 gggcttacca tctggcccca gtgctgcaat gataccgcga gaaccacgct caccggctcc
     1681 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     1741 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     1801 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     1861 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     1921 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     1981 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     2041 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     2101 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     2161 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     2221 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     2281 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     2341 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     2401 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     2461 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccac
//
