LOCUS       sequence-248988-       12025 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Over-express NLS-FLAG-cGAS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12025)
  AUTHORS   Gentili M, Lahaye X, Nadalin F, Nader GPF, Puig Lombardi E, Herve S,
            De Silva NS, Rookhuizen DC, Zueva E, Goudot C, Maurin M, Bochnakian 
            A, Amigorena S, Piel M, Fachinetti D, Londono-Vallejo A, Manel N
  TITLE     The N-Terminal Domain of cGAS Determines Preferential Association 
            with Centromeric DNA and Innate Immune Activation in the Nucleus.
  JOURNAL   Cell Rep. 2019 Feb 26;26(9):2377-2393.e13. doi: 
            10.1016/j.celrep.2019.01.105.
  PUBMED    30811988
REFERENCE   2  (bases 1 to 12025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.01"; journalName: "Cell Rep"; date: 
            "2019-02-26- 26"; volume: "26"; issue: "9"; pages: "2377-2393.e13"
COMMENT     Sequence Label: pTRIP-CMV-Puro-2A-NLS-FLAG-cGAS
FEATURES             Location/Qualifiers
     source          1..12025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3254..3557
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3558..3761
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3712..3732
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3801..4397
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3801..3820)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4297..4317
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4407..4463
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4491..4511
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4515..4538
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     LTR             6376..6556
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8084..8103)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8317..8336)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8436..8458
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8496..8514)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8582..8686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8687..8755,8756..9547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8905..8924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9718..10306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10207..10226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10460..10477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10547..10567)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10552..10881
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10732..10867
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 atttttaagt gtaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtatagtaat caattacggg gtcattagtt catagcccat
     3241 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     3301 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     3361 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     3421 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     3481 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     3541 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     3601 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     3661 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     3721 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga
     3781 tcccgcgggc tagcgccacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     3841 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     3901 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     3961 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     4021 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     4081 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     4141 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     4201 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     4261 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     4321 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     4381 cccgcaagcc cggtgccgga agcggagcta ctaacttcag cctgctgaag caggctggag
     4441 acgtggagga gaaccctgga cctggatcca ctagtggtgg cggcggaagt cccaagaaga
     4501 agcgcaaggt gatggactac aaagacgatg acgacaagat gcagccttgg cacggaaagg
     4561 ccatgcagag agcttccgag gccggagcca ctgcccccaa ggcttccgca cggaatgcca
     4621 ggggcgcccc gatggatccc aacgagtctc cggctgcccc cgaggccgcc ctgcctaagg
     4681 cgggaaagtt cggccccgcc aggaagtcgg gatcccggca gaaaaagagc gccccggaca
     4741 cccaggagag gccgcccgtc cgcgcaactg gggcccgcgc caaaaaggcc cctcagcgcg
     4801 cccaggacac gcagccgtct gacgccacca gcgcccctgg ggcagagggg ctggagcctc
     4861 ctgcggctcg ggagccggct ctttccaggg ctggttcttg ccgccagagg ggcgcgcgct
     4921 gctccacgaa gccaagaccc ccgcccgggc cctgggacgt gcccagcccc ggcctgccgg
     4981 tctcggcccc cattctcgta cggagggatg cggcgcctgg ggcctcgaag ctccgggcgg
     5041 ttttggagaa gttgaagctc agccgcgatg atatctccac ggcggcgggg atggtgaaag
     5101 gggttgtgga ccacctgctg ctcagactga agtgcgactc cgcgttcaga ggcgtcgggc
     5161 tgctgaacac cgggagctac tatgagcacg tgaagatttc tgcacctaat gaatttgatg
     5221 tcatgtttaa actggaagtc cccagaattc aactagaaga atattccaac actcgtgcat
     5281 attactttgt gaaatttaaa agaaatccga aagaaaatca tctgagtcag tttttagaag
     5341 gtgaaatatt atcagcttct aagatgctgt caaagtttag gaaaatcatt aaggaagaaa
     5401 ttaacgacat taaagataca gatgtcatca tgaagaggaa aagaggaggg agccctgctg
     5461 taacacttct tattagtgaa aaaatatctg tggatataac cctggctttg gaatcaaaaa
     5521 gtagctggcc tgctagcacc caagaaggcc tgcgcattca aaactggctt tcagcaaaag
     5581 ttaggaagca actacgacta aagccatttt accttgtacc caagcatgca aaggaaggaa
     5641 atggtttcca agaagaaaca tggcggctat ccttctctca catcgaaaag gaaattttga
     5701 acaatcatgg aaaatctaaa acgtgctgtg aaaacaaaga agagaaatgt tgcaggaaag
     5761 attgtttaaa actaatgaaa taccttttag aacagctgaa agaaaggttt aaagacaaaa
     5821 aacatctgga taaattctct tcttatcatg tgaaaactgc cttctttcac gtatgtaccc
     5881 agaaccctca agacagtcag tgggaccgca aagacctggg cctctgcttt gataactgcg
     5941 tgacatactt tcttcagtgc ctcaggacag aaaaacttga gaattatttt attcctgaat
     6001 tcaatctatt ctctagcaac ttaattgaca aaagaagtaa agaatttctg acaaagcaaa
     6061 ttgaatatga aagaaacaat gagtttccag tttttgatga attttgactc gagacctaga
     6121 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct
     6181 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc
     6241 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     6301 agggctaatt cactcccaac gaagacaaaa tcgtcgagag atgctgcata taagcagctg
     6361 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6421 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     6481 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     6541 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     6601 aagaaatgaa tatcagagag tgagaggcct tgacattata atagatttag caggaattga
     6661 actaggagtg gagcacacag gcaaagctgc agaagtactt ggaagaagcc accagagata
     6721 ctcacgattc tgcacatacc tggctaatcc cagatcctaa ggattacatt aagtttacta
     6781 acatttatat aatgatttat agtttaaagt ataaacttat ctaatttact attctgacag
     6841 atattaatta atcctcaaat atcataagag atgattacta ttatccccat ttaacacaag
     6901 aggaaactga gagggaaaga tgttgaagta attttcccac aattacagca tccgttagtt
     6961 acgactctat gatcttctga cacaaattcc atttactcct caccctatga ctcagtcgaa
     7021 tatatcaaag ttatggacat tatgctaagt aacaaattac ccttttatat agtaaatact
     7081 gagtagattg agagaagaaa ttgtttgcaa acctgaatag cttcaagaag aagagaagtg
     7141 aggataagaa taacagttgt catttaacaa gttttaacaa gtaacttggt tagaaaggga
     7201 ttcaaatgca taaagcaagg gataaatttt tctggcaaca agactataca atataacctt
     7261 aaatatgact tcaaataatt gttggaactt gataaaacta attaaatatt attgaagatt
     7321 atcaatatta taaatgtaat ttacttttaa aaagggaaca tagaaatgtg tatcattaga
     7381 gtagaaaaca atccttatta tcacaatttg tcaaaacaag tttgttatta acacaagtag
     7441 aatactgcat tcaattaagt tgactgcaga ttttgtgttt tgttaaaatt agaaagagat
     7501 aacaacaatt tgaattattg aaagtaacat gtaaatagtt ctacatacgt tcttttgaca
     7561 tcttgttcaa tcattgatcg aagttcttta tcttggaaga atttgttcca aagactctga
     7621 aataaggaaa acaatctatt atatagtctc acacctttgt tttactttta gtgatttcaa
     7681 tttaataatg taaatggtta aaatttattc ttctctgaga tcatttcaca ttgcagatag
     7741 aaaacctgag actggggtaa tttttattaa aatctaattt aatctcagaa acacatcttt
     7801 attctaacat caatttttcc agtttgatat tatcatataa agtcagcctt cctcatctgc
     7861 aggttccaca acaaaaatcc aaccaactgt ggatcaaaaa tattgggaaa aaattaaaaa
     7921 tagcaataca acaataaaaa aatacaaatc agaaaaacag cacagtataa caactttatt
     7981 tagcatttac aatctattag gtattataag taatctagcc agatcctcta cgccggacgc
     8041 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     8101 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     8161 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     8221 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     8281 tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata
     8341 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     8401 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     8461 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     8521 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     8581 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     8641 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     8701 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     8761 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     8821 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     8881 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     8941 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     9001 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     9061 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     9121 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     9181 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     9241 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     9301 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     9361 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     9421 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     9481 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     9541 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     9601 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     9661 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     9721 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     9781 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     9841 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     9901 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     9961 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
    10021 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
    10081 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
    10141 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
    10201 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
    10261 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
    10321 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
    10381 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
    10441 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
    10501 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag
    10561 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
    10621 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
    10681 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
    10741 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
    10801 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
    10861 ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag
    10921 aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga
    10981 aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa
    11041 cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt
    11101 cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga
    11161 acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg
    11221 cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac
    11281 cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt
    11341 gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg
    11401 cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt
    11461 gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg
    11521 ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac
    11581 ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc
    11641 aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt
    11701 ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat
    11761 ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg
    11821 cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga
    11881 tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga
    11941 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
    12001 tttaaagctc taaggtaaat ataaa
//
