LOCUS       sequence-248997-       10568 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  APEX2 fused to the mitochondrial nucleoid protein Twinkle.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10568)
  AUTHORS   Han S, Udeshi ND, Deerinck TJ, Svinkina T, Ellisman MH, Carr SA, 
            Ting AY
  TITLE     Proximity Biotinylation as a Method for Mapping Proteins Associated 
            with mtDNA in Living Cells.
  JOURNAL   Cell Chem Biol. 2017 Mar 16;24(3):404-414. doi: 
            10.1016/j.chembiol.2017.02.002. Epub 2017 Feb 23.
  PUBMED    28238724
REFERENCE   2  (bases 1 to 10568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2017.02.002"; journalName: "Cell Chem Biol"; 
            date: "2017-03-16- 16"; volume: "24"; issue: "3"; pages: "404-414"
COMMENT     Sequence Label: Twinkle-APEX2-V5
FEATURES             Location/Qualifiers
     source          1..10568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    595..619
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2725..3471
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             3472..3513
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(3518..3542)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3575..4163
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3628..3648)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4046..4057)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4235..4468
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4546..4667
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4583..4602)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4637..4656
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4707..4842
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4862..4881)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4863..4881)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4891..4908)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4891..4907)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4900..4922)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5049..5504
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5136..5155)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5346..5367
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5530..5634
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5635..5703,5704..6495)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5853..5872)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6666..7254
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7155..7174
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7408..7425
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7542..7563
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7578..7583,7584..7601,7602..7608)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7616..7632
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7621..7643
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7640..7656
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7640..7656
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7675..7695
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7677..7695
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7723..7949
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7950..8130
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8177..8302
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8795..9028
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9213..9257
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9432..9932
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9851..9869
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9944..10339
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    10399..10516
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtacaaaa aagcaggctt cgaaggagat agaaccatgt gggtcctcct ccgaagtggg
      661 taccccctcc gtatcttgtt acccctgcgt ggggagtgga tgggtcggag gggcctgccc
      721 cgaaacttgg ccccaggccc tcctcgcaga cgttacagga aggagactct ccaagccttg
      781 gatatgccag tgttgcctgt aactgcaact gaaatccgcc agtatttgcg ggggcatggg
      841 atccccttcc aggatggtca cagttgcctg cgggcactga gcccctttgc agagtcttca
      901 cagctcaaag gccagactgg tgttaccact tccttcagcc tcttcattga caagaccaca
      961 ggccactttc tctgcatgac cagcctagca gaagggagct gggaagactt ccaggccagc
     1021 gtggaggggc gaggggatgg ggccagggag gggtttctgc ttagcaaggc accagaattt
     1081 gaggacagcg aggaggtccg gaggatctgg aaccgagcaa tacctctctg ggagctgcct
     1141 gatcaggagg aggttcagct ggctgataca atgtttggcc ttaccaaggt tacagatgac
     1201 acactcaagc gtttcagtgt gcgatatctg cgacctgctc gcagtcttgt cttcccttgg
     1261 ttctcccctg ggggctcagg attacgaggc ctgaagctcc tagaggctaa atgccagggg
     1321 gatggagtga gctacgagga aaccactatt ccccgaccca gcgcctacca caatctgttt
     1381 ggattaccac tgattagtcg tcgagatgct gaggtggtac tgacgagtcg tgagcttgac
     1441 agcctggcct tgaaccagtc cacggggctg cctaccctta ctctaccccg aggaacgacc
     1501 tgcttacccc ctgccttact cccttacctg gaacagttcc ggcggattgt attctggttg
     1561 ggggatgacc ttcggtcctg ggaagccgcc aagttgtttg cacgaaaact gaaccccaaa
     1621 cgatgcttct tggtgcgacc aggagaccag caaccccgtc ccctggaggc cctgaacgga
     1681 ggcttcaatc tttctcgtat tcttcgtacc gccctgcctg cctggcacaa gtccatcgta
     1741 tctttccggc agcttcggga ggaggtgcta ggagaactgt caaatgtgga gcaagcagct
     1801 ggcctccgct ggagccgctt tccagacctc aatcgtatct tgaagggaca tcgaaagggc
     1861 gagctgacgg tcttcacagg gccaacaggc agtggaaaga cgacattcat cagtgagtat
     1921 gccctggatt tgtgttccca gggggtgaac acactgtggg gtagcttcga gatcagcaat
     1981 gtgagactag cccgggtcat gctgacacag tttgccgagg ggcggctgga agatcaactg
     2041 gacaaatatg atcactgggc tgaccgcttt gaggacctgc ccctctattt catgactttc
     2101 catggacagc aaagcatcag gactgtaata gatacaatgc aacatgcagt ctacgtctat
     2161 gacatttgtc atgtgatcat cgacaacctg cagttcatga tgggtcacga gcagctgtcc
     2221 acagacagga tcgcagctca agactacatc atcggggtct ttcggaagtt tgcaacagac
     2281 aataactgcc atgtgacact ggtcattcac ccccggaaag aggatgatga caaggaactg
     2341 cagacagcgt ccatttttgg ctcagccaaa gcaagccagg aagcagacaa tgttctgatc
     2401 ctgcaggaca ggaagctggt aaccgggcca gggaaacggt atctgcaggt gtccaagaac
     2461 cgctttgatg gagatgtagg tgtcttcccg cttgagttca acaagaactc cctcaccttc
     2521 tccattccac caaagaacaa ggcccggctc aagaagatca aggatgacac tggaccagtg
     2581 gccaaaaagc cctcttctgg caaaaagggg gctacgacac agaactctga gatttgctca
     2641 ggccaggccc ccactcccga ccagccagac acctccaagc gttcaaaggg atccaagggc
     2701 tcgggctcga cctcgggctc gggcggaaag tcttacccaa ctgtgagtgc tgattaccag
     2761 gacgccgttg agaaggcgaa gaagaagctc agaggcttca tcgctgagaa gagatgcgct
     2821 cctctaatgc tccgtttggc attccactct gctggaacct ttgacaaggg cacgaagacc
     2881 ggtggaccct tcggaaccat caagcaccct gccgaactgg ctcacagcgc taacaacggt
     2941 cttgacatcg ctgttaggct tttggagcca ctcaaggcgg agttccctat tttgagctac
     3001 gccgatttct accagttggc tggcgttgtt gccgttgagg tcacgggtgg acctaaggtt
     3061 ccattccacc ctggaagaga ggacaagcct gagccaccac cagagggtcg cttgcccgat
     3121 cccactaagg gttctgacca tttgagagat gtgtttggca aagctatggg gcttactgac
     3181 caagatatcg ttgctctatc tgggggtcac actattggag ctgcacacaa ggagcgttct
     3241 ggatttgagg gtccctggac ctctaatcct cttattttcg acaactcata cttcacggag
     3301 ttgttgagtg gtgagaagga aggtctcctt cagctacctt ctgacaaggc tcttttgtct
     3361 gaccctgtat tccgccctct cgttgacaaa tatgcagcgg acgaagatgc cttctttgct
     3421 gattacgctg aggctcacca aaagctttcc gagcttgggt ttgctgatgc cggcaagccc
     3481 atccccaacc ccctgctggg cctggacagc acctaagacc cagctttctt gtacaaagtg
     3541 gtgctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt acaaaatttg
     3601 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     3661 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     3721 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     3781 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     3841 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     3901 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     3961 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     4021 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     4081 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     4141 ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta
     4201 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     4261 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     4321 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     4381 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     4441 ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt
     4501 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     4561 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     4621 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     4681 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     4741 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     4801 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     4861 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     4921 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     4981 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     5041 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     5101 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     5161 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     5221 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     5281 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     5341 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     5401 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     5461 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     5521 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5581 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     5641 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     5701 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     5761 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     5821 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     5881 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     5941 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     6001 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     6061 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     6121 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     6181 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     6241 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     6301 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     6361 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     6421 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     6481 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     6541 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6601 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6661 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6721 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6781 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     6841 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6901 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6961 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7021 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     7081 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7141 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7201 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     7261 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     7321 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     7381 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     7441 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     7501 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     7561 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     7621 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     7681 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
     7741 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
     7801 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
     7861 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
     7921 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct
     7981 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     8041 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     8101 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     8161 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     8221 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     8281 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     8341 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     8401 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     8461 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     8521 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     8581 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     8641 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     8701 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     8761 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     8821 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     8881 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     8941 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     9001 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     9061 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     9121 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     9181 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     9241 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     9301 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     9361 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     9421 cgaggggacc cttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct
     9481 ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt
     9541 ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc
     9601 ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa
     9661 cggaagccgc acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag
     9721 cgcgccgacc gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca
     9781 gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc
     9841 tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc
     9901 tcgttgaccg aatcaccgac ctctctcccc agggggtacc accatggcca agcctttgtc
     9961 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc
    10021 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt
    10081 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc
    10141 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat
    10201 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc
    10261 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc
    10321 tggttatgtg tgggagggcc tgcagctgca gtagtaagaa ttctagatct tgagacaaat
    10381 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
    10441 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
    10501 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt ggcgccggct
    10561 cgaggggg
//
