LOCUS       sequence-249032-       10957 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of FGFR3 S249C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10957)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-FGFR3-S249C
FEATURES             Location/Qualifiers
     source          1..10957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5894..5918)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5971..6012
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6033..6600
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6050..6602
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6194..6211)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6421..6440
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6614..6616,6617..6619,6620..7333)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6614..6616,6617..6619,6620..7330)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6659..6680)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6920..6939)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7267..7288
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7347..7935
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7400..7420)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7818..7829)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8010..8243
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8362..8380)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8448..8552
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8553..8621,8622..9413)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8771..8790)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9584..10172
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10073..10092
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10326..10343
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10583..10604
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10619..10624,10625..10642,10643..10649)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10657..10673
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10662..10684
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10681..10697
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10681..10697
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10747..10766
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc agaagggccg tcaaggccca ccatgggcgc ccctgcctgc
     3481 gccctcgcgc tctgcgtggc cgtggccatc gtggccggcg cctcctcgga gtccttgggg
     3541 acggagcagc gcgtcgtggg gcgagcggca gaagtcccgg gcccagagcc cggccagcag
     3601 gagcagttgg tcttcggcag cggggatgct gtggagctga gctgtccccc gcccgggggt
     3661 ggtcccatgg ggcccactgt ctgggtcaag gatggcacag ggctggtgcc ctcggagcgt
     3721 gtcctggtgg ggccccagcg gctgcaggtg ctgaatgcct cccacgagga ctccggggcc
     3781 tacagctgcc ggcagcggct cacgcagcgc gtactgtgcc acttcagtgt gcgggtgaca
     3841 gacgctccat cctcgggaga tgacgaagac ggggaggacg aggctgagga cacaggtgtg
     3901 gacacagggg ccccttactg gacacggccc gagcggatgg acaagaagct gctggccgtg
     3961 ccggccgcca acaccgtccg cttccgctgc ccagccgctg gcaaccccac tccctccatc
     4021 tcctggctga agaacggcag ggagttccgc ggcgagcacc gcattggagg catcaagctg
     4081 cggcatcagc agtggagcct ggtcatggaa agcgtggtgc cctcggaccg cggcaactac
     4141 acctgcgtcg tggagaacaa gtttggcagc atccggcaga cgtacacgct ggacgtgctg
     4201 gagcgctgcc cgcaccggcc catcctgcag gcggggctgc cggccaacca gacggcggtg
     4261 ctgggcagcg acgtggagtt ccactgcaag gtgtacagtg acgcacagcc ccacatccag
     4321 tggctcaagc acgtggaggt gaacggcagc aaggtgggcc cggacggcac accctacgtt
     4381 accgtgctca agacggcggg cgctaacacc accgacaagg agctagaggt tctctccttg
     4441 cacaacgtca cctttgagga cgccggggag tacacctgcc tggcgggcaa ttctattggg
     4501 ttttctcatc actctgcgtg gctggtggtg ctgccagccg aggaggagct ggtggaggct
     4561 gacgaggcgg gcagtgtgta tgcaggcatc ctcagctacg gggtgggctt cttcctgttc
     4621 atcctggtgg tggcggctgt gacgctctgc cgcctgcgca gcccccccaa gaaaggcctg
     4681 ggctccccca ccgtgcacaa gatctcccgc ttcccgctca agcgacaggt gtccctggag
     4741 tccaacgcgt ccatgagctc caacacacca ctggtgcgca tcgcaaggct gtcctcaggg
     4801 gagggcccca cgctggccaa tgtctccgag ctcgagctgc ctgccgaccc caaatgggag
     4861 ctgtctcggg cccggctgac cctgggcaag ccccttgggg agggctgctt cggccaggtg
     4921 gtcatggcgg aggccatcgg cattgacaag gaccgggccg ccaagcctgt caccgtagcc
     4981 gtgaagatgc tgaaagacga tgccactgac aaggacctgt cggacctggt gtctgagatg
     5041 gagatgatga agatgatcgg gaaacacaaa aacatcatca acctgctggg cgcctgcacg
     5101 cagggcgggc ccctgtacgt gctggtggag tacgcggcca agggtaacct gcgggagttt
     5161 ctgcgggcgc ggcggccccc gggcctggac tactccttcg acacctgcaa gccgcccgag
     5221 gagcagctca ccttcaagga cctggtgtcc tgtgcctacc aggtggcccg gggcatggag
     5281 tacttggcct cccagaagtg catccacagg gacctggctg cccgcaatgt gctggtgacc
     5341 gaggacaacg tgatgaagat cgcagacttc gggctggccc gggacgtgca caacctcgac
     5401 tactacaaga agacaaccaa cggccggctg cccgtgaagt ggatggcgcc tgaggccttg
     5461 tttgaccgag tctacactca ccagagtgac gtctggtcct ttggggtcct gctctgggag
     5521 atcttcacgc tggggggctc cccgtacccc ggcatccctg tggaggagct cttcaagctg
     5581 ctgaaggagg gccaccgcat ggacaagccc gccaactgca cacacgacct gtacatgatc
     5641 atgcgggagt gctggcatgc cgcgccctcc cagaggccca ccttcaagca gctggtggag
     5701 gacctggacc gtgtccttac cgtgacgtcc accgacgagt acctggacct gtcggcgcct
     5761 ttcgagcagt actccccggg tggccaggac acccccagct ccagctcctc aggggacgac
     5821 tccgtgtttg cccacgacct gctgcccccg gccccaccca gcagtggggg ctcgcggacg
     5881 tagggcctca tgggcccagc tttcttgtac aaagtggttg atatccagca cagtggcggc
     5941 cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt
     6001 ctcgattcta cgcgtaccgg ttagtaatga gatccctccc ccccccctaa cgttactggc
     6061 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     6121 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     6181 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     6241 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     6301 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     6361 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     6421 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     6481 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     6541 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     6601 tatggccaca catatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     6661 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     6721 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     6781 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     6841 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     6901 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     6961 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     7021 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     7081 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     7141 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     7201 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     7261 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     7321 gctgtacaag taaatcgata gatcctaatc aacctctgga ttacaaaatt tgtgaaagat
     7381 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     7441 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     7501 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     7561 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     7621 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     7681 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     7741 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     7801 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     7861 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     7921 gggccgcctc cccgcctgag atcctttaag accaatgact tacaaggcag ctgtagatct
     7981 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     8041 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     8101 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     8161 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     8221 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     8281 acttgcaaag aaatgaatat cagagagtga gaggcccggg ttaattaagg aaagggctag
     8341 atcattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     8401 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     8461 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     8521 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     8581 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     8641 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     8701 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     8761 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     8821 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     8881 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     8941 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     9001 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     9061 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     9121 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     9181 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     9241 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     9301 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     9361 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     9421 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     9481 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     9541 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     9601 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     9661 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     9721 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     9781 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     9841 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     9901 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     9961 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    10021 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    10081 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    10141 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    10201 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    10261 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    10321 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
    10381 ccgcgcgttg gccgattcat taatgcagca agctcatggc tgactaattt tttttattta
    10441 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
    10501 tggaggccta ggcttttgca aaaagctccc cgtggcacga caggtttccc gactggaaag
    10561 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    10621 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    10681 caggaaacag ctatgacatg attacgaatt tcacaaataa agcatttttt tcactgcatt
    10741 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
    10801 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
    10861 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
    10921 attgtatttg ttaaatatgt actacaaact tagtagt
//
