LOCUS       sequence-249040-       12092 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A cytidine deaminase-mediated base-editing plasmid in Acinetobacter 
            baumannii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12092)
  AUTHORS   Wang Y, Wang Z, Chen Y, Hua X, Yu Y, Ji Q
  TITLE     A Highly Efficient CRISPR-Cas9-Based Genome Engineering Platform in 
            Acinetobacter baumannii to Understand the H2O2-Sensing Mechanism of 
            OxyR.
  JOURNAL   Cell Chem Biol. 2019 Sep 17. pii: S2451-9456(19)30277-6. doi: 
            10.1016/j.chembiol.2019.09.003.
  PUBMED    31548010
REFERENCE   2  (bases 1 to 12092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Chem 
            Biol. 2019 Sep 17. pii: S2451-9456(19)30277-6. doi: 
            10.1016/j.chembiol.2019.09.003."
COMMENT     Sequence Label: pBECAb-apr
FEATURES             Location/Qualifiers
     source          1..12092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(1..1335,1336..1422))
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     CDS             1967..2653
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(2702..2725,2726..2728,2729..6805)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     promoter        6850..6884
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        6902..6977
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(7010..7026)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7010..7026)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7085..7101)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7085..7101)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7885..8076
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    8178..8317
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8332..8349)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8503..9091)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8583..8602)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(10039..10143)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10211..10229
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     11946..11968
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11960..11977
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11961..11977
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12014..12030
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     12015..12031
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttatttgtta actgttaatt gtccttgttc aaggatgctg tctttgacaa cagatgtttt
       61 cttgcctttg atgttcagca ggaagctagg cgcaaacgtt gattgtttgt ctgcgtagaa
      121 tcctctgttt gtcatatagc ttgtaatcac gacattgttt cctttcgctt gaggtacagc
      181 gaagtgtgag taagtaaagg ttacatcgtt aggatcaaga tccattttta acacaaggcc
      241 agttttgttc agcggcttgt atgggccagt taaagaatta gaaacataac caagcatgta
      301 aatatcgtta gacgtaatgc cgtcaatcgt catttttgat ccgcgggagt cagtgaacag
      361 ataccatttg ccgttcattt taaagacgtt cgcgcgttca atttcatctg ttactgtgtt
      421 agatgcaatc agcggtttca tcactttttt cagtgtgtaa tcatcgttta gctcaatcat
      481 accgagagcg ccgtttgcta actcagccgt gcgtttttta tcgctttgca gaagtttttg
      541 actttcttga cggaagaatg atgtgctttt gccatagtat gctttgttaa ataaagattc
      601 ttcgccttgg tagccatctt cagttccagt gtttgcttca aatactaagt atttgtggcc
      661 tttatcttct acgtagtgag gatctctcag cgtatggttg tcgcctgagc tgtagttgcc
      721 ttcatcgatg aactgctgta cattttgata cgtttttccg tcaccgtcaa agattgattt
      781 ataatcctct acaccgttga tgttcaaaga gctgtctgat gctgatacgt taacttgtgc
      841 agttgtcagt gtttgtttgc cgtaatgttt accggagaaa tcagtgtaga ataaacggat
      901 ttttccgtca gatgtaaatg tggctgaacc tgaccattct tgtgtttggt cttttaggat
      961 agaatcattt gcatcgaatt tgtcgctgtc tttaaagacg cggccagcgt ttttccagct
     1021 gtcaatagaa gtttcgccga ctttttgata gaacatgtaa atcgatgtgt catccgcatt
     1081 tttaggatct ccggctaatg caaagacgat gtggtagccg tgatagtttg cgacagtgcc
     1141 gtcagcgttt tgtaatggcc agctgtccca aacgtccagg ccttttgcag aagagatatt
     1201 tttaattgtg gacgaatcga actcaggaac ttgatatttt tcattttttt gctgttcagg
     1261 gatttgcagc atatcatggc gtgtaatatg ggaaatgccg tatgtttcct tatatggctt
     1321 ttggttcgtt tctttcgcaa acgcttgagt tgcgcctcct gccagcagtg cggtagtaaa
     1381 ggttaatact gttgcttgtt ttgcaaactt tttgatgttc atcgttcatg tctccttttt
     1441 tatgtactgt gttagcggtc tgcttcttcc agccctcctg tttgaagatg gcaagttagt
     1501 tacgcacaat aaaaaaagac ctaaaatatg taaggggtga cgccaaagta tacactttgc
     1561 cctttacaca ttttaggtct tgcctgcttt atcagtaaca aacccgcgcg atttactttt
     1621 cgacctcatt ctattagact ctcgtttgga ttgcaactgg tctattttcc tcttttgttt
     1681 gatagaaaat cataaaagga tttgcagact acgggcctaa agaactaaaa aatctatctg
     1741 tttcttttca ttctctgtat tttttatagt ttctgttgca tgggcataaa gttgcaagct
     1801 tgcaaacatg ctttgggtaa acatgtaggt attaacgtca atgcgacaat agtagcattg
     1861 attaaatgag aattagtaag tgttttactt actaaatttt atttaaccta aaaatgaacc
     1921 acctggatgt gtgggattaa aaagtgaaga gaggaggaca tatcacatga gtagcgaaac
     1981 cggtccggtt gcagtggatc cgaccctgcg ccgtcgcatt gaaccgcacg agtttgaagt
     2041 gttctttgat ccgcgcgagc tgcgcaaaga aacttgcctg ctgtacgaga ttaactgggg
     2101 tggccgccat agcatctggc gccataccag ccagaacacc aataagcacg tggaagtgaa
     2161 ttttattgaa aaatttacca ccgagcgcta cttctgccct aatacccgct gcagcatcac
     2221 ctggtttctg agctggagcc cgtgcggcga atgtagtcgc gccattaccg agttcctgag
     2281 ccgctatccg catgtgaccc tgttcatcta catcgcccgt ctgtaccatc acgccgatcc
     2341 gcgcaatcgc caaggtctgc gtgatctgat tagcagcggt gtgaccatcc agatcatgac
     2401 cgaacaagag agcggctatt gctggcgcaa cttcgtgaac tattctccga gcaacgaagc
     2461 ccactggccg cgttatccgc atctgtgggt gcgcctgtat gtgctggagc tgtactgcat
     2521 catcctgggc ctgccgcctt gcctgaatat tctgcgccgt aaacagccgc aactgacatt
     2581 cttcaccatc gcactgcaga gctgccatta tcagcgcctg ccgccgcaca ttttatgggc
     2641 caccggtctg aaaagcggta gtgaaactcc gggcacaagc gaaagcgcaa ccccggaaag
     2701 tgataagaaa tactcaatag gcttagctat cggcacaaat agcgtcggat gggcggtgat
     2761 cactgatgaa tataaggttc cgtctaaaaa gttcaaggtt ctgggaaata cagaccgcca
     2821 cagtatcaaa aaaaatctta taggggctct tttatttgac agtggagaga cagcggaagc
     2881 gactcgtctc aaacggacag ctcgtagaag gtatacacgt cggaagaatc gtatttgtta
     2941 tctacaggag attttttcaa atgagatggc gaaagtagat gatagtttct ttcatcgact
     3001 tgaagagtct tttttggtgg aagaagacaa gaagcatgaa cgtcatccta tttttggaaa
     3061 tatagtagat gaagttgctt atcatgagaa atatccaact atctatcatc tgcgaaaaaa
     3121 attggtagat tctactgata aagcggattt gcgcttaatc tatttggcct tagcgcatat
     3181 gattaagttt cgtggtcatt ttttgattga gggagattta aatcctgata atagtgatgt
     3241 ggacaaacta tttatccagt tggtacaaac ctacaatcaa ttatttgaag aaaaccctat
     3301 taacgcaagt ggagtagatg ctaaagcgat tctttctgca cgattgagta aatcaagacg
     3361 attagaaaat ctcattgctc agctccccgg tgagaagaaa aatggcttat ttgggaatct
     3421 cattgctttg tcattgggtt tgacccctaa ttttaaatca aattttgatt tggcagaaga
     3481 tgctaaatta cagctttcaa aagatactta cgatgatgat ttagataatt tattggcgca
     3541 aattggagat caatatgctg atttgttttt ggcagctaag aatttatcag atgctatttt
     3601 actttcagat atcctaagag taaatactga aataactaag gctcccctat cagcttcaat
     3661 gattaaacgc tacgatgaac atcatcaaga cttgactctt ttaaaagctt tagttcgaca
     3721 acaacttcca gaaaagtata aagaaatctt ttttgatcaa tcaaaaaacg gatatgcagg
     3781 ttatattgat gggggagcta gccaagaaga attttataaa tttatcaaac caattttaga
     3841 aaaaatggat ggtactgagg aattattggt gaaactaaat cgtgaagatt tgctgcgcaa
     3901 gcaacggacc tttgacaacg gctctattcc ccatcaaatt cacttgggtg agctgcatgc
     3961 tattttgaga agacaagaag acttttatcc atttttaaaa gacaatcgtg agaagattga
     4021 aaaaatcttg acttttcgaa ttccttatta tgttggtcca ttggcgcgtg gcaatagtcg
     4081 ttttgcatgg atgactcgga agtctgaaga aacaattacc ccatggaatt ttgaagaagt
     4141 tgtcgataaa ggtgcttcag ctcaatcatt tattgaacgc atgacaaact ttgataaaaa
     4201 tcttccaaat gaaaaagtac taccaaaaca tagtttgctt tatgagtatt ttacggttta
     4261 taacgaattg acaaaggtca aatatgttac tgaaggaatg cgaaaaccag catttctttc
     4321 aggtgaacag aagaaagcca ttgttgattt actcttcaaa acaaatcgaa aagtaaccgt
     4381 taagcaatta aaagaagatt atttcaaaaa aatagaatgt tttgatagtg ttgaaatttc
     4441 aggagttgaa gatagattta atgcttcatt aggtacctac catgatttgc taaaaattat
     4501 taaagataaa gattttttgg ataatgaaga aaatgaagat atcttagagg atattgtttt
     4561 aacattgacc ttatttgaag atagggagat gattgaggaa agacttaaaa catatgctca
     4621 cctctttgat gataaggtga tgaaacagct taaacgtcgc cgttatactg gttggggacg
     4681 tttgtctcga aaattgatta atggtattag ggataagcaa tctggcaaaa caatattaga
     4741 ttttttgaaa tcagatggtt ttgccaatcg caattttatg cagctgatcc atgatgatag
     4801 tttgacattt aaagaagaca ttcaaaaagc acaagtgtct ggacaaggcg atagtttaca
     4861 tgaacatatt gcaaatttag ctggtagccc tgctattaaa aaaggtattt tacagactgt
     4921 aaaagttgtt gatgaattgg tcaaagtaat ggggcggcat aagccagaaa atatcgttat
     4981 tgaaatggca cgtgaaaatc agacaactca aaagggccag aaaaattcgc gagagcgtat
     5041 gaaacgaatc gaagaaggta tcaaagaatt aggaagtcag attcttaaag agcatcctgt
     5101 tgaaaatact caattgcaaa atgaaaagct ctatctctat tatctccaaa atggaagaga
     5161 catgtatgtg gaccaagaat tagatattaa tcgtttaagt gattatgatg tcgatcacat
     5221 tgttccacaa agtttcctta aagacgattc aatagacaat aaggtcttaa cgcgttctga
     5281 taaaaatcgt ggtaaatcgg ataacgttcc aagtgaagaa gtagtcaaaa agatgaaaaa
     5341 ctattggaga caacttctaa acgccaagtt aatcactcaa cgtaagtttg ataatttaac
     5401 gaaagctgaa cgtggaggtt tgagtgaact tgataaagct ggttttatca aacgccaatt
     5461 ggttgaaact cgccaaatca ctaagcatgt ggcacaaatt ttggatagtc gcatgaatac
     5521 taaatacgat gaaaatgata aacttattcg agaggttaaa gtgattacct taaaatctaa
     5581 attagtttct gacttccgaa aagatttcca attctataaa gtacgtgaga ttaacaatta
     5641 ccatcatgcc catgatgcgt atctaaatgc cgtcgttgga actgctttga ttaagaaata
     5701 tccaaaactt gaatcggagt ttgtctatgg tgattataaa gtttatgatg ttcgtaaaat
     5761 gattgctaag tctgagcaag aaataggcaa agcaaccgca aaatatttct tttactctaa
     5821 tatcatgaac ttcttcaaaa cagaaattac acttgcaaat ggagagattc gcaaacgccc
     5881 tctaatcgaa actaatgggg aaactggaga aattgtctgg gataaagggc gagattttgc
     5941 cacagtgcgc aaagtattgt ccatgcccca agtcaatatt gtcaagaaaa cagaagtaca
     6001 gacaggcgga ttctccaagg agtcaatttt accaaaaaga aattcggaca agcttattgc
     6061 tcgtaaaaaa gactgggatc caaaaaaata tggtggtttt gatagtccaa cggtagctta
     6121 ttcagtccta gtggttgcta aggtggaaaa agggaaatcg aagaagttaa aatccgttaa
     6181 agagttacta gggatcacaa ttatggaaag aagttccttt gaaaaaaatc cgattgactt
     6241 tttagaagct aaaggatata aggaagttaa aaaagactta atcattaaac tacctaaata
     6301 tagtcttttt gagttagaaa acggtcgtaa acggatgctg gctagtgccg gagaattaca
     6361 aaaaggaaat gagctggctc tgccaagcaa atatgtgaat tttttatatt tagctagtca
     6421 ttatgaaaag ttgaagggta gtccagaaga taacgaacaa aaacaattgt ttgtggagca
     6481 gcataagcat tatttagatg agattattga gcaaatcagt gaattttcta agcgtgttat
     6541 tttagcagat gccaatttag ataaagttct tagtgcatat aacaaacata gagacaaacc
     6601 aatacgtgaa caagcagaaa atattattca tttatttacg ttgacgaatc ttggagctcc
     6661 cgctgctttt aaatattttg atacaacaat tgatcgtaaa cgatatacgt ctacaaaaga
     6721 agttttagat gccactctta tccatcaatc catcactggt ctttatgaaa cacgcattga
     6781 tttgagtcag ctaggaggtg actgatggct ggttggcgta ctgttgtggt aaataccaag
     6841 cttgatatct tgacagctag ctcagtccta ggtataatac tagtcgagac cattggtctc
     6901 agttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
     6961 tggcaccgag tcggtgcttt ttttgatatc gaattcctgc agcccggggg atccactagt
     7021 tctagagcgg ccgccaccgc ggtggagctc atcccaatgg cgcgccgagc ttggctcgag
     7081 catggtcata gctgtttcct gctcactcat taggcaccgg gatctcgacc gatgcccttg
     7141 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca
     7201 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc
     7261 attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta
     7321 ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc
     7381 ggcgagaagc aggccattat cgccggcatg gcggccccac gggtgcgcat gatcgtgctc
     7441 ctgtcgttga ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca
     7501 ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca
     7561 acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc
     7621 tgcaccatta tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct
     7681 acatctgtat taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc
     7741 atccataccg ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca
     7801 gtaacccgta tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga
     7861 aatccccctt acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc
     7921 cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat
     7981 gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc
     8041 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     8101 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     8161 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
     8221 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccattgc ggtgtgaaat
     8281 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
     8341 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8401 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8461 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8521 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8581 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8641 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8701 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8761 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8821 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8881 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8941 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9001 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9061 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9121 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9181 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9241 tgagtaaact tggtctgaca gtcagccaat cgactggcga gcggcatcgc attcttcgca
     9301 tcccgcctct ggcggatgca ggaagatcaa cggatctcgg cccagttgac ccagggctgt
     9361 cgccacaatg tcgcgggagc ggatcaaccg agcaaaggca tgaccgactg gaccttcctt
     9421 ctgaaggctc ttctccttga gccacctgtc cgccaaggca aagcgctcac agcagtggtc
     9481 attctcgaga taatcgacgc gtaccaactt gccatcctga agaatggtgc agtgtctcgg
     9541 caccccatag ggaacctttg ccatcaactc ggcaagatgc agcgtcgtgt tggcatcgtg
     9601 tcccacgccg aggagaagta cctgcccatc gagttcatgg acacgggcga ccgggcttgc
     9661 aggcgagtga ggtggcaggg gcaatggatc agagatgatc tgctctgcct gtggccccgc
     9721 tgccgcaaag gcaaatggat gggcgctgcg ctttacattt ggcaggcgcc agaatgtgtc
     9781 agagacaact ccaaggtccg gtgtaacggg cgacgtggca ggatcgaacg gctcgtcgtc
     9841 cagacctgac cacgagggca tgacgagcgt ccctcccgga cccagcgcag cacgcagggc
     9901 ctcgatcagt ccaagtggcc catcttcgag gggccggacg ctacggaagg agctgtggac
     9961 cagcagcaca ccgccggggg taaccccaag gttgagaagc tgaccgatga gctcggcttt
    10021 tcgccattcg tattgcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10081 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10141 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
    10201 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
    10261 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
    10321 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
    10381 taactatgcg gcatcagagc agattgtact gagagtgcac cataggattt taacattttg
    10441 cgttgttcca aaagttatca acagcctaga acgtcatagg aagcgattac agacacttta
    10501 tagctatcag catgggaaca taagggcagg atgaaatatg ggtcttaaaa cgcaaatggt
    10561 gaggttttag aggtattttt tgaagatgat taaggcggtt tgtttttaaa atttttggcg
    10621 gctctcaggc tgcttacatt ttaaccagtt cagtgaaaag ttctttttca gcaaatttct
    10681 gtttagcacc atagctaaaa cttgcgtgga acatattaag aattgaccga aatgacacaa
    10741 tctcaattat attttttttg aaaagttttc tttatcaaat attttaaatc attgatttat
    10801 atataagtat acattcattt taataattaa tctttattta acaatgattt atctatattc
    10861 aattgtttaa ttattcttac taatattatc tctatatcaa tattttttat ttaaaaacat
    10921 atgtttagta gtgcttttga ttaaagtacc agagggaggg agcagagctg aatgggaaat
    10981 actcacccta gagcgattct taaaaatcac cctaaagtat tcccattcga tgtaccgtcg
    11041 gtcggtcgct ttcgcatcag ggatgacatc actgtatcaa gctgccactg ttatgattac
    11101 gattgatagc accgcctgaa cacgctcata accgccaatt aaatgactac tcattgcgtc
    11161 cgctactctg ttcagttccc ctagtaatag cgtttttccg atgtgtgcta gcgtcactgt
    11221 acctcatcac ccacacatgg acagattgag ttacagacat tggctaaatt tttggggtct
    11281 atgcttgaca aagcgagtta aaaccttaga atttaagaca ggtacattaa gcccctgtgg
    11341 tgaaatcatt agcggtcgtc aaaccttaat gctttcgatt gccgatatgt cagtatcgaa
    11401 gatctgtacc ccataaatta cggtaaagcc ccaagcaatt gcaaggggct tttatctttt
    11461 ttaacaaaaa aaatttataa atcaggattt tataacacta aataatccaa agtacatgag
    11521 taagtcatga ccactcctgc gatgtgtgta gcactctgag tatccgtatt atatcagtga
    11581 tgtcatatac aaccacataa tgcggatgta tcactaactc cctagtatct ttctgtctgc
    11641 ctgtgcgccc catcagtcga ttatcaacaa gtaaatctgt cttttcttca attaaatcat
    11701 caatttcaat agctgctata gggttgcgtt cttcaagata atcatagata tttctacgat
    11761 cttggctatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    11821 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    11881 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    11941 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgacgcgta ttgggatggt
    12001 accgggcccc ccctcgaggt cgacggtatc gatacggcat cagagcagat tgtactgaga
    12061 gtgcaccata atcggcattt tcttttgcgt tt
//
