LOCUS       sequence-249041-        2198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly DE part ECK120029600 (378x-strength 
            natural terminator; see DOI: 10.1038/nmeth.2515). Please see 
            Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2198)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T12m
FEATURES             Location/Qualifiers
     source          1..2198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     71..89
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(93..136)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    139..160
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    280..300
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(306..323)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(477..1065)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(557..576)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1236..2027,2028..2096))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1859..1878
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(2097..2198,1..3))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
       61 aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt
      121 cgctaaggat gatttctgga attcgcggcc gcttctagag actagtgggt ctcaaggttt
      181 cagccaaaaa acttaagacc gccggtcttg tccactacct tgcagtaatg cggtggacag
      241 gatcggcggt tttcttttct cttctcaagc ttagagacct actagtagcg gccgctgcag
      301 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      361 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      421 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      481 cacaggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      541 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      601 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      661 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      721 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      781 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      841 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      901 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      961 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1021 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1081 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1141 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1201 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1261 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1321 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgcgac
     1381 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1441 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1501 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1561 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1621 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1681 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1741 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1801 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1861 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1921 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1981 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     2041 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     2101 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     2161 tttgaatgta tttagaaaaa taaacaaata ggggttcc
//
