LOCUS       sequence-249049-        6220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For in vitro translation of human ELL2 lacking COOH region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Martincic K, Alkan SA, Cheatle A, Borghesi L, Milcarek C
  TITLE     Transcription elongation factor ELL2 directs immunoglobulin 
            secretion in plasma cells by stimulating altered RNA processing.
  JOURNAL   Nat Immunol. 2009 Oct;10(10):1102-9. doi: 10.1038/ni.1786. Epub 2009
            Sep 13.
  PUBMED    19749764
REFERENCE   2  (bases 1 to 6220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.1786"; 
            journalName: "Nat Immunol"; date: "2009-10"; volume: "10"; issue: 
            "10"; pages: "1102-9"
COMMENT     Sequence Label: 564aa ELL2 bluescript
FEATURES             Location/Qualifiers
     source          1..6220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(52..85)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(641..652)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(3410..3428)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3449..3465)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3449..3465)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3462..3484)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3473..3489
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3497..3503,3504..3521,3522..3527))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3542..3563
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3680..3697)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3851..4439)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3931..3950)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4610..5401,5402..5470))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5233..5252
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5471..5575)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5601..6056)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5738..5759)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5950..5969
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6183..6205
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6197..6214
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6198..6214
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatacgact cactataggg cgaattggag ctccaccgcg gtggcggccg cataacttcg
       61 tatagcatac attatacgaa gttatggatc aggccaaatc ggccgagctc gaattcgtcg
      121 agagcgggat aagttggcgc gtcacctaga cgtcagcctc ttacctctcg gacgtcccgt
      181 cccaggagca aaactctagt gactttgggc ccgcccccgt gagagtggag ggcggccgat
      241 gagggcggtt ctgaagggaa caagaccaat gagacgagcc cggggcgggg cgcggggccg
      301 gagggtgggg cgggtggcgg cggtggcagt ccgaggtctg gctccgcgca gtatatgaca
      361 gtacgtcagc agcgggatgg ccctagcagt ggcggcggct gcagaagccc aagcagccgc
      421 ggccgcagtg gaggctagag ccggagcggc ggcggcggcg gcaccccggg gagtttaaga
      481 tggcggcggg ggggacaggg ggcctgcggg aggagcagcg ctatgggctg tcgtgcggac
      541 ggctggggca ggacaacatc accgtactgc atgtgaagct caccgagacg gcgatccggg
      601 cgctcgagac ttaccagagc cacaagaatt taattccttt tcgaccttca atccagttcc
      661 aaggactcca cgggcttgtc aaaattccca aaaatgatcc cctcaatgaa gttcataact
      721 ttaactttta tttgtcaaat gtgggcaaag acaaccctca gggcagcttt gactgcatcc
      781 agcaaacatt ctccagctct ggagcctccc agctcaattg cctgggattt atacaagata
      841 aaattacagt gtgtgcaaca aacgactcgt atcagatgac acgagaaaga atgacccagg
      901 cagaggagga atcccgcaac cgaagcacaa aagttatcaa acccggtgga ccatatgtag
      961 ggaaaagagt gcaaattcgg aaagcacctc aagctgtttc agatacagtt cctgagagga
     1021 aaaggtcaac ccccatgaac cctgcaaata caattcgaaa gacacatagc agcagcacca
     1081 tctctcagag gccatacagg gacagggtga ttcacttact ggccctgaag gcctacaaga
     1141 aaccggagct acttgctaga ctccagaaag atggtgtcaa tcaaaaagac aagaactccc
     1201 tgggagcaat tctgcaacag gtagccaatc tgaattctaa ggacctctca tataccttaa
     1261 aggattatgt ttttaaagag cttcaaagag actggcctgg atacagtgaa atagacagac
     1321 ggtcattgga gtcagtgctc tctagaaaac taaatccgtc tcagaatgct acaggcacca
     1381 gccgttcaga atctcctgta tgttctagta gagatgctgt atcttctcct cagaaacggc
     1441 ttttggattc agagtttatt gatcctttaa tgaataaaaa agcccgaata tctcacctga
     1501 cgaacagagt accaccaaca ctaaatggtc atttgaatcc caccagtgaa aaatcggctg
     1561 caggcctccc actgccccct gcggctgctg ccatccccac ccctccaccg ctgccttcaa
     1621 cctatctgcc catctcacat cctcctcaga ttgtaaattc taactccaac tcccctagca
     1681 ctccagaagg ccgggggact caagacctac ctgttgacag ttttagtcaa aacgatagta
     1741 tctatgagga ccagcaagac aaatatacct ctaggacttc tctggaaacc ttaccccctg
     1801 gttccgttct actaaagtgt ccaaagccta tggaagaaaa ccattcaatg tctcacaaaa
     1861 agtccaaaaa gaagtctaaa aaacataagg aaaaggacca aataaaaaag cacgacattg
     1921 agactattga ggaaaaggag gaagatctta agagagaaga ggaaattgcc aagctaaata
     1981 actccagtcc aaattccagt ggaggagtta aagaggattg cactgcctcc atggaacctt
     2041 cagcaattga actcccagat tatttgataa aatatatcgc tatcgtctcc tatgagcaac
     2101 gccagaatta taaggatgac ttcaatgcag agtatgatga gtacagagct ttgccaggat
     2161 ggagactgta gctagaagat ttatcaaact agatgcacaa agaaagcgcc tttctccagg
     2221 ctcaaaagag tatcagaatg ttcatgaaga agtcttacaa gaatatcaga agataaagca
     2281 gtctagtccc aattaccatg aagaaaaata cagatgtgaa tatcttcata acaagctggc
     2341 tcacatcaaa aggctaatag gtgaatttga ccaacagcaa gcagagtcat ggtcctagaa
     2401 ctctgcttgg accagaagat gtgaataaac ttaagcttgg aaaaaaaaaa aaaaaaaaaa
     2461 aaaaaaaaac gcgtaacccc ccattgcaat ggttgcctta aggtgtttgc tagttgtgta
     2521 catagtgtgg ttaatcatta gctacactgc ttcccacttg attagagcaa tgggaagcat
     2581 actgtggcct accagcatct ggaagtgtgt gctcgatctg tatgtgtgca gaggtggtgt
     2641 ggatgtgagc gtgcatgaag gaaaaaaagc tgctactcct agtaggccaa acgctcaggt
     2701 taaacaactg acgagtgtta ctgtagggtg tttttttgtt tttttgtttt ttttcttcta
     2761 tcaaattgct acttttgttg tggaagacaa aagcatttcc atttcaacga gtttgtcagc
     2821 tttattaatg ttgggcaaaa attgatatgt catgaaaatg aaacagatct atagttttgg
     2881 gacaaaatta taaaatgaaa tgtgtaggta acctatttat atactgctat aaagtatttt
     2941 ttgaagagag atatgcaaag aagctattac ctacataaga ggtatattta aagatttttt
     3001 ttttttcatc ctggtgccag gaatataaaa aagagtggat atatttaacc ataacatact
     3061 gtgattcatc aaacagcaca aactttcatt tcatggagtt tatctgttga cattgattta
     3121 aactgtcact tgttttatca tgtgggaaca taagttatgt ggtcaaaaat ataaggattt
     3181 tgaattaatg ttgattcaag ttgtattgtc ttttcaaagt gctgccagtt gaaaagggaa
     3241 gcattatgtt tacaaatctg ttttgaaatg tttgccaaaa ttttggtagt gtctttaata
     3301 aagatgtttg tctccagcat ccagaaaaaa aaaaaaaaaa ctctccagcg ctggatccgg
     3361 ccataagggc ctgatccttc gagggggggc ccggtaccag cttttgttcc ctttagtgag
     3421 ggttaatttc gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     3481 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     3541 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     3601 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     3661 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     3721 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     3781 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     3841 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     3901 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     3961 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     4021 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     4081 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     4141 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     4201 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     4261 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     4321 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     4381 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     4441 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     4501 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     4561 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     4621 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     4681 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     4741 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     4801 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     4861 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     4921 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     4981 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     5041 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     5101 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     5161 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     5221 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     5281 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     5341 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     5401 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     5461 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     5521 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     5581 ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     5641 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     5701 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     5761 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     5821 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     5881 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     5941 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     6001 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca
     6061 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     6121 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     6181 ttcccagtca cgacgttgta aaacgacggc cagtgaattg
//
