LOCUS       sequence-249050-       14870 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14870)
  AUTHORS   Zhao B, Li L, Lu Q, Wang LH, Liu CY, Lei Q, Guan KL
  TITLE     Angiomotin is a novel Hippo pathway component that inhibits YAP 
            oncoprotein.
  JOURNAL   Genes Dev. 2011 Jan 1;25(1):51-63.
  PUBMED    21205866
REFERENCE   2  (bases 1 to 14870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2011-01-1"; volume: "25(1)"; pages: "51-63"
COMMENT     Sequence Label: Lenti GFP-AMOT p130
FEATURES             Location/Qualifiers
     source          1..14870
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGREQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1793..1811
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1849..1871)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1971..1990
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2204..2223
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        2513..2531
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2513..2530
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             4010..4643
                     /label=5' LTR
                     /note="5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4846..5434
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4952..4963
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5361..5381
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5976..5993)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(7523..7543)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             complement(join(8702..9412,9413..9415,9416..9418))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(8702..9412,9413..9415,9416..9418))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8744..8765)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     9093..9112
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9352..9373
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9489..9513)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(9510..9713)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9539..9559)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9714..10017
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    10081..10198
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(10460..10504)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10689..10922
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    11415..11540
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             11587..12220
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    12388..12522
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12412..12431)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     12466..12485
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(12947..12967)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          13097..13162
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(14296..14625)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      14310..14445
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     14610..14630
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(14700..14717)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tctcgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     1801 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     1861 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     1921 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     1981 gcagattgta ctgagagtgc accattcgac gctctccctt atgcgactcc tgcattagga
     2041 agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca
     2101 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac
     2161 aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat
     2221 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga
     2281 ggatctggct agcgatgacc ctgctgattg gttcgctgac catttccggg tgcgggacgg
     2341 cgttaccaga aactcagaag gttcgtccaa ccaaaccgac tctgacggca gtttacgaga
     2401 gagatgatag ggtctgcttc agtaagccag atgctacaca attaggcttg tacatattgt
     2461 cgttagaacg cggctacaat taatacataa ccttatgtat catacacata cgatttaggt
     2521 gacactatag aatacacgga attaattcta gattacttat aatacctaat agattgtaaa
     2581 tgctaaataa agttgttata ctgtgctgtt tttctgattt gtattttttt attgttgtat
     2641 tgctattttt aattttttcc caatattttt gatccacagt tggttggatt tttgttgtgg
     2701 aacctgcaga tgaggaaggc tgactttata tgataatatc aaactggaaa aattgatgtt
     2761 agaataaaga tgtgtttctg agattaaatt agattttaat aaaaattacc ccagtctcag
     2821 gttttctatc tgcaatgtga aatgatctca gagaagaata aattttaacc atttacatta
     2881 ttaaattgaa atcactaaaa gtaaaacaaa ggtgtgagac tatataatag attgttttcc
     2941 ttatttcaga gtctttggaa caaattcttc caagataaag aacttcgatc aatgattgaa
     3001 caagatgtca aaagaacgta tgtagaacta tttacatgtt actttcaata attcaaattg
     3061 ttgttatctc tttctaattt taacaaaaca caaaatctgc agtcaactta attgaatgca
     3121 gtattctact tgtgttaata acaaacttgt tttgacaaat tgtgataata aggattgttt
     3181 tctactctaa tgatacacat ttctatgttc cctttttaaa agtaaattac atttataata
     3241 ttgataatct tcaataatat ttaattagtt ttatcaagtt ccaacaatta tttgaagtca
     3301 tatttaaggt tatattgtat agtcttgttg ccagaaaaat ttatcccttg ctttatgcat
     3361 ttgaatccct ttctaaccaa gttacttgtt aaaacttgtt aaatgacaac tgttattctt
     3421 atcctcactt ctcttcttct tgaagctatt caggtttgca aacaatttct tctctcaatc
     3481 tactcagtat ttactatata aaagggtaat ttgttactta gcataatgtc cataactttg
     3541 atatattcga ctgagtcata gggtgaggag taaatggaat ttgtgtcaga agatcataga
     3601 gtcgtaacta acggatgctg taattgtggg aaaattactt caacatcttt ccctctcagt
     3661 ttcctcttgt gttaaatggg gataatagta atcatctctt atgatatttg aggattaatt
     3721 aatatctgtc agaatagtaa attagataag tttatacttt aaactataaa tcattatata
     3781 aatgttagta aacttaatgt aatccttagg atctgggatt agccaggtat gtgcagaatc
     3841 gtgagtatct ctggtggctt cttccaagta cttctgcagc tttgcctgtg tgctccactc
     3901 ctagttcaat tcctgctaaa tctattataa tgtcaaggcc tctcactctc tgatattcat
     3961 ttctttgcaa gttataaata ctgaataata agatgacatg aactactact gctagagatt
     4021 ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg
     4081 gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct
     4141 agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc agtacaagca
     4201 aaaagcagct gcttatatgc agcatctgag ggctcgccac tccccagtcc cgcccaggcc
     4261 acgcctccct ggaaagtccc cagcggaaag tcccttgtag caagctcgat gtcagcagtt
     4321 cttgaagtac tccggatgca gctctcgggc catgtgatga aatgctaggc ggctgtcaaa
     4381 cctccactct aatacttctc tctccgggtc atccatccca tgcaggctca cagggtgtaa
     4441 caagcgggtg ttctctcctt cattggcttc ttctaccttc tcttgctcaa ctggtactag
     4501 cttgtagcac catccaaagg tcagtggata tctgatccct ggccctggtg tgtagttctg
     4561 ccaatcaggg aagtagcctt gtgtgtggta gatccacaga tcaaggatat cttgtcttcg
     4621 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     4681 agctgccttg taagtcattg gtcttaaagg tacctgaggt gtgactggaa aacccacctc
     4741 ctcctcctct tgtgcttcta gccaggcaca atcagcattg gtagctgctg tattgctact
     4801 tgtgattgct ccatgttttt ctaggtctcg actgcagaat tccaggcggg gaggcggccc
     4861 aaagggagat ccgactcgtc tgagggcgaa ggcgaagacg cggaagaggc cgcagagccg
     4921 gcagcaggcc gcgggaagga aggtccgctg gattgagggc cgaagggacg tagcagaagg
     4981 acgtcccgcg cagaatccag gtggcaacac aggcgagcag ccatggaaag gacgtcagct
     5041 tccccgacaa caccacggaa ttgtcagtgc ccaacagccg agcccctgtc cagcagcggg
     5101 caaggcaggc ggcgatgagt tccgccgtgg caatagggag ggggaaagcg aaagtcccgg
     5161 aaaggagctg acaggtggtg gcaatgcccc aaccagtggg ggttgcgtca gcaaacacag
     5221 tgcacaccac gccacgttgc ctgacaacgg gccacaactc ctcataaaga gacagcaacc
     5281 aggatttata caaggaggag aaaatgaaag ccatacggga agcaatagca tgatacaaag
     5341 gcattaaagc agcgtatcca catagcgtaa aaggagcaac atagttaaga ataccagtca
     5401 atctttcaca aattttgtaa tccagaggtt gattctcgag ttagatgaga tattccacca
     5461 tctctgcatc aggctcttgt cccaggatct gaatgggagt ttttctttcc agagtattgg
     5521 agtggaacac aggcccatct gttttgtctg gattgcaggt caaacttggt atagacaaac
     5581 gatgtggtcc aggaccggta gctggacttg caggaacctc agcctgagcc acagctggag
     5641 ttggagttgg agctggagtt ggagccacag ccggagctga agttggtgcc tgagtctgag
     5701 caggagcaga agcctgagcc gctgctggag ctgggaccgg aaccagagcc ggagctggaa
     5761 ctggagccgg agcagctgga gcaacctgaa cagcagcagc agcagcagca gaaggagcaa
     5821 cggcagcagc tgaggcaaca gaggcagcag ctggaatctg accagcagca gctggagaaa
     5881 cagcagcagc agtagcagcg gcagtggctg gagacggggc agcagcagca gctggagcag
     5941 cagcagcagc aacagcaact ggagcagcag ctaccatggt ggtggtgatg gtggcagcag
     6001 tggcagtgat ggcggcagca gtggcggcag cagcaactgg agcaggaaca gagatgggag
     6061 caacagctgc agttttgttc gattccgtcc cacgttcagt ttgggtactg cagtctcggc
     6121 tgtctgtctt ggagtgagcc gagagcaggg gagtcgaggg tggcacaggc gagggtgtgg
     6181 aagggacata ttcagcacgg tagtctccac ccaggagaat gcctaggctc cccttccagc
     6241 tcttgtcgtc tcgcttttct tccatgatgg gggagccagt cagggtggat gagtgggaga
     6301 gcaagcctga tccagcattg gaaatggaca tcagagactt cgctggccgc atgcacgaca
     6361 gctgctctgt cttgctcggc tccttccggg aacgctgctg gagtactttg atcatggcat
     6421 ccttctcaat aatctgggca tggagggtct taatcctgcc ctccatgtca aggcaacgct
     6481 tattggccat caagatttct tcctcctctt tctggatgcg agcttctaga gctgtgtcat
     6541 agctggtgtt aggagagtga ctgatgactg ttgtgtccct ctgagcagcc acagttgcag
     6601 cagcatccag agcaaaatgt ctcatcacat tctcctccaa atatttctgc tcccactttg
     6661 tcatatcagc ttccagagcc agaatcctct cctctttctc ccgaaggagc tccatcagtg
     6721 cagcagcatt gtattctgaa acgttggtgg gctgacagtt gccctgacgc tgctggattc
     6781 tcagggattc cagttccctc tccagtcgtg tccggagacg gtgctctagc tgctcacgtt
     6841 tttcacatgc tgcctggagc tgtacaaggg cctgctgcat cttctccacc ttgtcaacgt
     6901 acacttgctt ctttttcagc tcttcttcca gctttaccac cttggcctgg gcattactca
     6961 gggcctgatc tcggatttcg atgtgtcgtc tttggtcctc attggtagaa cgggcagtgg
     7021 ccagctccgc ttccagcttc tccttctcac gctggctttc tttatttttt gcaaagagct
     7081 gcgagatggt ttttctggtg tcctctgacc cctcatattc cttctctgca agctgcttgt
     7141 tggcagtctc tagacgctct ctcagatccc tgttgaaatc atgcatcctc cgaatctcgc
     7201 cctctagctt gtttctcatg gctttctcta gggcctctct tttggaggat gacttcacga
     7261 ggttctcata tgcctccgag acgcgctgga tttctgtctc caccttctgc agtcttgcca
     7321 ccttctcata gcatccttcc aactcttgcc tcaagttccg gttctcgtct gagaggatct
     7381 caaccatctg ctgggctctg gaaacaatgg caaaagggtc tgctggcact ggctgataag
     7441 aagcagagga tggctgagcc cgaggcatag ctgaataggc ttctcctggc tgctgctgtg
     7501 gctgctgctg ctgctgttgt tggtggtgat ggtgatgatg gtgctgctgc tgctgttgct
     7561 gctgctgctg actcaggcca ggttgggaga gacggtaatg atccccctgg tgagcctgat
     7621 taggaaggaa atgctgctgc ggcctaggca ggtgagctga atggtctccc tggtttggga
     7681 ccaaggggtg tcgggcagga gacaatctag tggatggtgg agattgtagc aagggcaagg
     7741 accccccaga ggtcagagaa gaagtagggc tgtgaggctg cgagttcctg gctgacaatg
     7801 gcaatgagat gtcctgcgct ggcctggctg ctccatactc agggggatgc tgatacctca
     7861 tcagttgccc ctctgttctc cctggctggt tcaggcgatg ctcactatag aagtgccctg
     7921 gctcttgggg cttgcacact acagattggg gtggcatgcc cttgaaggga tattctggtg
     7981 gggggcctcg gtgttccatg cccttcaggc tatgctggtt aggaagtggc ccttgggcct
     8041 tgtagaattc actggaactt gtgggattct tgtagaggtc attgggttgt ggtggggaga
     8101 ggggagcact ggtaacaggt ggatgggcct taactccact ggtggccagt gacatctgca
     8161 ttagtcgttc actcaaggaa cggacatgcc cttgcttaag gtctctgagt ccctcatctt
     8221 ggtgcatctt tccaggattg agccgctgaa ctgatgggcg tccctcagtc ctcatcttct
     8281 ggttggtgac tccagtgaca tagaaggcag ctccaacact ggcatgctgt tggccccgaa
     8341 agtactggga ctggaccttg gcttcttcat aggtcgggag ttcttcatta ttttgcattc
     8401 gaggagacag ctgcttctcc atgatgaggt tttctgactg gatttcctgc ccctggggtt
     8461 cttgtcgagc agcatgagcc acaagctgtt ggtggtggtc ttggggactc aacacatcac
     8521 tctgagggcc cgggttccca ctgccactgg ggaaaggagg gccattccct gtggcttgct
     8581 ggtgtatggc aagcaggctg cgattctcac taggattgcc atagcgaagc tgctcttgta
     8641 gcaaacgctg caataccgtg gtccctccac ttggctgttc gtcagaattt ctcatggatc
     8701 ccttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga
     8761 ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt
     8821 ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg
     8881 cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt
     8941 tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt
     9001 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
     9061 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga
     9121 agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct
     9181 tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg
     9241 tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt
     9301 aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt
     9361 ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg
     9421 tggtagatcc tctagtagag tcggtgtctt ctatggaggt caaaacagcg tggatggcgt
     9481 ctccaggcga tctgacggtt cactaaacga gctctgctta tatagacctc ccaccgtaca
     9541 cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt
     9601 tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc
     9661 cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta
     9721 atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac
     9781 tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc
     9841 atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga
     9901 cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat
     9961 gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg
    10021 aactcccaag cttatcgatg aattccaaac tggatctctg ctgtccctgt aataaacccg
    10081 aaaattttga atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat
    10141 tatgtctact attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaatc
    10201 gataccgtcg agatccgttc actaatcgaa tggatctgtc tctgtctctc tctccacctt
    10261 cttcttctat tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg
    10321 ataatggtga atatccctgc ctaactctat tcactataga aagtacagca aaaactattc
    10381 ttaaacctac caagcctcct actatcatta tgaataattt tatataccac agccaatttg
    10441 ttatgttaaa ccaattccac aaacttgccc atttatctaa ttccaataat tcttgttcat
    10501 tcttttcttg ctggttttgc gattcttcaa ttaaggagtg tattaagctt gtgtaattgt
    10561 taatttctct gtcccactcc atccaggtcg tgtgattcca aatctgttcc agagatttat
    10621 tactccaact agcattccaa ggcacagcag tggtgcaaat gagttttcca gagcaacccc
    10681 aaatccccag gagctgttga tcctttaggt atctttccac agccaggatt cttgcctgga
    10741 gctgcttgat gccccagact gtgagttgca acagatgctg ttgcgcctca atagccctca
    10801 gcaaattgtt ctgctgctgc actataccag acaataattg tctggcctgt accgtcagcg
    10861 tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg aacaaagctc
    10921 ctattcccac tgctcttttt tctctctgca ccactcttct ctttgccttg gtgggtgcta
    10981 ctcctaatgg ttcaattttt actactttat atttatataa ttcacttctc caattgtccc
    11041 tcatatctcc tcctccaggt ctgaagatca gcggccgctt gctgtgcggt ggtcttactt
    11101 ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat ctctatcctt
    11161 tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc ttctgatcct
    11221 gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga tgtttctaac
    11281 aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat atgttttaat
    11341 ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga tctaattctc
    11401 ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc gctagtcaaa
    11461 atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc
    11521 cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc cctgttcggg
    11581 cgccactgct agagattttc cacactgact aaaagggtct gagggatctc tagttaccag
    11641 agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct
    11701 taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga
    11761 gagacccagt acaggcaaaa agcagctgct tatatgcagg atctgagggc tcgccactcc
    11821 ccagtcccgc ccaggccacg cctccctgga aagtccccag cggaaagtcc cttgtagcaa
    11881 gctcgatatc agcagttctt gaagtactcc ggatgcagct ctcgggccac gtgatgaaat
    11941 gctaggcggc tgtcaaacct ccactctaac acttctctct ccgggtcatc catcccatgc
    12001 aggctcacag ggtgtaacaa gctggtgttc tctcctttat tggcctcttc taccttatct
    12061 ggctcaactg gtactagctt gtagcaccat ccaaaggtca gtggatatct gacccctggc
    12121 cctggtgtgt agttctgcta atcagggaag tagccttgtg tgtggtagat ccacagatca
    12181 aggatatctt gtcttctttg ggagtgaatt agcccttcca actactaagt ttgtagtaca
    12241 tatttaacaa atacaatttc tttaaaatga aaataattca gaggaatcac aggtttagag
    12301 taaatgaaac cacaggtaat tggcagtggt aatagggtat ggggtgggaa gtttgggatg
    12361 attttggtta gcttgagtta tccagttgat ccagacatga taagatacat tgatgagttt
    12421 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
    12481 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
    12541 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
    12601 tacaaatgtg gtatggctga ttatgatctc tagtcaaggc actatacatc aaatattcct
    12661 tattaacccc tttacaaatt aaaaagctaa aggtacacaa tttttgagca tagttattaa
    12721 tagcagacac tctatgcctg tgtggagtaa gaaaaaacag tatgttatga ttataactgt
    12781 tatgcctact tataaaggtt acagaatatt tttccataat tttcttgtat agcagtgcag
    12841 ctttttcctt tgtggtgtaa atagcaaagc aagcaagagt tctattacta aacacagcat
    12901 gactcaaaaa acttagcaat tctgaaggaa agtccttggg gtcttctacc tttctcttct
    12961 tttttggagg agtagaatgt tgagagtcag cagtagcctc atcatcacta gatggcattt
    13021 cttctgagca aaacaggttt tcctcattaa aggcattcca ccactgctcc cattcatcag
    13081 ttccataggt tggaatctaa aatacacaaa caattagaat cagtagttta acacattata
    13141 cacttaaaaa ttttatattt accttagagc tttaaatctc tgtaggtagt ttgtccaatt
    13201 atgtcacacc acagaagtaa ggttccttca caaagatccg gggcccactc ataaatccag
    13261 ttgccgccac ggtagccaat caccgtatcg tataaatcat cgtcggtacg ttcggcatcg
    13321 ctcatcacaa tacgtgcctg gacgtcgagg atttcgcgtg ggtcaatgcc gcgccagatc
    13381 cacatcagac ggttaatcat gcgataccag tgagggatgg ttttaccatc aagggccgac
    13441 tgcacaggcg gttgtgcgcc gtgattaaag cggcggacta gcgtcgaggt ttcaggatgt
    13501 ttaaagcggg gtttgaacag ggtttcgctc aggtttgcct gtgtcatgga tgcagcctcc
    13561 agaatactta ctggaaacta ttgtaacccg cctgaagtta aaaagaacaa cgcccggcag
    13621 tgccaggcgt tgaaaagatt agcgaccgga gattggcggg acgaatacga cgcccatatc
    13681 ccacggctgt tcaatccagg tatcttgcgg gatatcaaca acatagtcat caaccagcgg
    13741 acgaccagcc ggttttgcga agatggtgac aaagtgcgct tttggataca tttcacgaat
    13801 cgcaaccgca gtaccaccgg tatccaccag gtcatcaata acgatgaagc cttcgccatc
    13861 gccttctgcg cgtttcagca ctttaagctc gcgctggttg tcgtgatcgt agctggaaat
    13921 acaaacggta tcgacatgac gaatacccag ttcacgcgcc agtaacgcac ccggtacaga
    13981 ccgccacggc ttacggcaat aatgcctttc cattgttcag aaggcatcag tcggcttgcg
    14041 agtttacgtg catggatctg caacatgtcc caggtgacga tgtatttttc gctcatgtga
    14101 agtgtcccag cctgtttatc tacggcttaa aaagtgttcg aggggaaaat aggttgcgcg
    14161 agattataga gatctggcgc actaaaaacc agtatttcac atgagtccgc gtctttttac
    14221 gcactgcctc tccctgacgc gggataaagt ggtattctca aacatatctc gcaagcctgt
    14281 cttgtgtcca agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
    14341 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
    14401 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
    14461 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
    14521 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
    14581 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagctgcat
    14641 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
    14701 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
    14761 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
    14821 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
//
