LOCUS       sequence-249051-        1905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Kruppel Associated Box repression domain as a Type 3a part 
            to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3a_014
FEATURES             Location/Qualifiers
     source          1..1905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             206..391
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     terminator      complement(435..469)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(540..1199)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1114..1133
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1381..1905,1..64)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1870..1889
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
       61 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
      121 catgttcttt cctgcgttat cccctgattc tgtggataac cgtagtcggt ctcatatgga
      181 tgctaagtca ctaactgcct ggtcccggac actggtgacc ttcaaggatg tatttgtgga
      241 cttcaccagg gaggagtgga agctgctgga cactgctcag cagatcgtgt acagaaatgt
      301 gatgctggag aactataaga acctggtttc cttgggttat cagcttacta agccagatgt
      361 gatcctccgg ttggagaagg gagaagagcc cggcggttcc ggcggagggt cgatgggttc
      421 ttgagaccag accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
      481 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
      541 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
      601 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
      661 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
      721 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgaaacgaa aaacatattc
      781 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
      841 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
      901 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
      961 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
     1021 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     1081 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     1141 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     1201 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     1261 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga ccaaaatccc
     1321 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     1381 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     1441 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     1501 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     1561 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     1621 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     1681 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     1741 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     1801 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     1861 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtt
//
