LOCUS       sequence-249055-        2568 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Absisic acid insensitive 1 as a Type 3a part to be used in 
            the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3a_016
FEATURES             Location/Qualifiers
     source          1..2568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      56..644
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     545..564
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1678..1712)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1783..2442)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2357..2376
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
       61 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
      121 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
      181 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
      241 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      301 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      361 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      421 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      481 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      541 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      601 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      661 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      721 cccctgattc tgtggataac cgtagtcggt ctcatatgac gcgtgtgcct ttgtatggtt
      781 ttacttcgat ttgtggaaga agacctgaga tggaagctgc tgtttcgact ataccaagat
      841 tccttcaatc ttcctctggt tcgatgttag atggtcggtt tgatcctcaa tccgccgctc
      901 atttcttcgg tgtttacgac ggccatggcg gttctcaggt agcgaactat tgtagagaga
      961 ggatgcattt ggctttggcg gaggagatag ctaaggagaa accgatgctc tgcgatggtg
     1021 atacgtggct ggagaagtgg aagaaagctc ttttcaactc gttcctgaga gttgactcgg
     1081 agattgagtc agttgcgccg gaaacggttg ggtcaacgtc ggtggttgcc gttgttttcc
     1141 catctcacat cttcgtcgct aactgcggtg actctagagc cgttctttgc cgcggcaaaa
     1201 ctgcacttcc attatccgtt gaccataaac cggatagaga agatgaagct gcgaggattg
     1261 aagccgcagg agggaaagtg attcagtgga atggagctcg tgttttcggt gttctcgcca
     1321 tgtcgagatc cattggcgat agatacttga aaccatccat cattcctgat ccggaagtga
     1381 cggctgtgaa gagagtaaaa gaagatgatt gtctgatttt ggcgagtgac ggggtttggg
     1441 atgtaatgac ggatgaagaa gcgtgtgaga tggcaaggaa gcggattctc ttgtggcaca
     1501 agaaaaacgc ggtggctggg gatgcatcgt tgctcgcgga tgagcggaga aaggaaggga
     1561 aagatcctgc ggcgatgtcc gcggctgagt atttgtcaaa gctggcgata cagagaggaa
     1621 gcaaagacaa cataagtgtg gtggtggttg atttgaaggg ttcttgagac cagaccaata
     1681 aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt
     1741 actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca
     1801 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacaaac
     1861 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
     1921 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
     1981 ggtgaaactc acccagggat tggctgaaac gaaaaacata ttctcaataa accctttagg
     2041 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
     2101 ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
     2161 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
     2221 acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
     2281 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
     2341 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg
     2401 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
     2461 tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa
     2521 gttggaacct cttacgtgcc cgatcaatca tgaccaaaat cccttaac
//
