LOCUS       sequence-249056-        1941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Gibberellin Insensitive as a Type 3a part to be used in the 
            MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3a_017
FEATURES             Location/Qualifiers
     source          1..1941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      236..824
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     725..744
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             948..965
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(1231..1265)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(join(1336..1941,1..54))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1910..1929
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
       61 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
      121 attatggtga aagttggaac ctcttacgtg cccgatcaat catgaccaaa atcccttaac
      181 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
      241 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
      301 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
      361 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
      421 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      481 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      541 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      601 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      661 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      721 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      781 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      841 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      901 cccctgattc tgtggataac cgtagtcggt ctcatatgaa gagagatcat catcatcatc
      961 atcatcaaga taagaagact atgatgatga atgaagaaga cgacggtaac ggcatggatg
     1021 agcttctagc tgttcttggt tacaaggtta ggtcatccga aatggctgat gttgctcaga
     1081 aactcgagca gcttgaagtt atgatgtcta atgttcaaga agacgatctt tctcaactcg
     1141 ctactgagac tgttcactat aatccggcgg agctttacac gtggcttgat tctatgctca
     1201 ccgaccttaa tggttcttga gaccagacca ataaaaaacg cccggcggca accgagcgtt
     1261 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc
     1321 gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
     1381 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
     1441 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     1501 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     1561 aacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     1621 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     1681 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     1741 ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag
     1801 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     1861 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
     1921 aaatgcctca aaatgttctt t
//
