LOCUS       sequence-249060-        3553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CFP fluorescent reporter for GadX.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Rossi NA, El Meouche I, Dunlop MJ
  TITLE     Forecasting cell fate during antibiotic exposure using stochastic 
            gene expression.
  JOURNAL   Commun Biol. 2019 Jul 11;2:259. doi: 10.1038/s42003-019-0509-0. 
            eCollection 2019.
  PUBMED    31312728
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2019-07-11"; volume: "2:259. doi"; pages: " 
            10.1038/s42003-019-0509-0. eCollection 2019"
COMMENT     Sequence Label: PgadX-cfp  (ColE1)
FEATURES             Location/Qualifiers
     source          1..3553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1..12
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      785..856
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      872..899
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(930..1518)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1010..1029)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1601..1695)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1726..2520)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1837..1856)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2447..2466
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 aaagaggaga aaggtaccgc atgactagca aaagaagcaa aggtgaagaa ctgttcactg
       61 gtgttgttcc aattctggtt gaactggatg gtgatgttaa tggtcacaaa ttttctgtct
      121 ctggtgaggg tgaaggtgat gcaacctacg gtaaactgac cctgaaattt atttgcacta
      181 ctggtaaact gcctgttccg tggccaaccc tggtcactac tctgacttgg ggtgttcaat
      241 gctttgctcg ttacccagat cacatgaaac agcatgactt tttcaagtct gccatgccgg
      301 aaggttatgt tcaggaacgt actatctttt tcaaagatga cggtaactac aagacccgtg
      361 ctgaagtcaa gtttgaaggt gataccctgg ttaatcgtat cgagctgaaa ggtattgatt
      421 ttaaagaaga tggtaacatt ctgggtcaca aactggaata caacgctatt tctgataatg
      481 tatacatcac tgctgacaaa caaaagaatg gtatcaaagc taatttcaaa attcgtcaca
      541 acattgaaga tggtagcgtt caactggcag accattatca acaaaatact ccaattggcg
      601 atggccctgt cctgctgcca gacaaccatt acctgtccac ccaatctcgt ctgtctaaag
      661 atccgaacga aaagcgcgat cacatggtcc tgctggagtt tgtaaccgct gctggtatta
      721 ccctgggcat ggatgaactg tataaataat agggatccaa actcgagtaa ggatctccag
      781 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      841 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
      901 ttatacctag gggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
      961 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1021 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1081 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1141 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1201 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1261 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1321 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     1381 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1441 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1501 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1561 aacgaaaact cacgttaagg gattttggtc atgagcttgg attctcacca ataaaaaacg
     1621 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
     1681 ctatcaacag gagtccaagc gagctctcga accccagagt cccgctcaga agaactcgtc
     1741 aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag
     1801 gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat
     1861 gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc
     1921 attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc
     1981 gtcgggcatg cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc
     2041 ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat
     2101 gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg
     2161 cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc
     2221 ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag
     2281 cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg
     2341 cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc
     2401 tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc
     2461 gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat
     2521 gcgaaacgat cctcatcctg tctcttgatc agatcatgat cccctgcgcc atcagatcct
     2581 tggcggcaag aaagccatcc agtttacttt gcagggcttc ccaaccttac cagagggcgc
     2641 cccagctggc aattccgacg tcggtgccta atgagtgagc taacttacat taattgcgtt
     2701 gcgcgttgac tactgggtgg tcaaattggt acttttgcca tcaacttctc ccgcccggcg
     2761 ggtcaggtaa ttgcacagta ctatgaattc ctgcgcctcg gtcgtgaagg ctataccaaa
     2821 gtacagaacg cctcttacca ggttgccgct tatctggcgg atgaaatcgc caaactgggg
     2881 ccgtatgagt tcatctgtac gggtcgcccg gacgaaggca tcccggcggt ttgcttcaaa
     2941 ctgaaagatg gtgaagatcc gggatacacc ctgtacgacc tctctgaacg tctgcgtctg
     3001 cgcggctggc aggttccggc cttcactctc ggcggtgaag ccaccgacat cgtggtgatg
     3061 cgcattatgt gtcgtcgcgg cttcgaaatg gactttgctg aactgttgct ggaagactac
     3121 aaagcctccc tgaaatatct cagcgatcac ccgaaactgc agggtattgc ccagcagaac
     3181 agctttaaac acacctgata acataacgtt gtaaaaaccg aatgcccagc ctttaaaaaa
     3241 acagctgggc attcggttgc ttattaatcg caataatata ttggctgtct attcatcgtg
     3301 ttgataagat atatgttata tgaatgttta ttaaatgtag cgattacctt taatgtcacg
     3361 aaaagggatt atttgcttac tattaatttc cctgtgttct taaacacgtt tttgtattat
     3421 aaatccagtc atcctgcccg tagaacatca cacattatca tcctgttctc ccgcttcgtt
     3481 taaatttatt tatcaatcaa tttgacttaa gagggcggcg tgctacatta ataaacagtg
     3541 gatccttaac ttt
//
