LOCUS       sequence-249061-        2235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Absisic acid receptor as a Type 3a part to be used in the 
            MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3a_019
FEATURES             Location/Qualifiers
     source          1..2235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      236..824
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     725..744
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1525..1559)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(join(1630..2235,1..54))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2204..2223
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
       61 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
      121 attatggtga aagttggaac ctcttacgtg cccgatcaat catgaccaaa atcccttaac
      181 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
      241 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
      301 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
      361 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
      421 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      481 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      541 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      601 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      661 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      721 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      781 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      841 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      901 cccctgattc tgtggataac cgtagtcggt ctcatatggg tgggggcgcg ccaactcaag
      961 acgaattcac ccaactctcc caatcaatcg ccgagttcca cacgtaccaa ctcggtaacg
     1021 gccgttgctc atctctccta gctcagcgaa tccacgcgcc gccggaaaca gtatggtccg
     1081 tggttagacg tttcgatagg ccacagattt acaaacactt catcaaaagc tgtaacgtga
     1141 gtgaagattt cgagatgcga gtgggatgca cgcgcgacgt gaacgtgata agtggattac
     1201 cggcgaatac ttctcgggag agattagatc tgttggacga tgatcggaga gtgactgggt
     1261 ttagtataac cggtggtgaa cataggctga ggaattataa atcggttacg acggttcata
     1321 gatttgagaa agaagaagaa gaagaaagga tctggaccgt tgttttggaa tcttatgttg
     1381 ttgatgtacc ggaaggtaat tcggaggaag atacgagatt gtttgctgat acggttatta
     1441 gattgaatct tcagaaactt gcttcgatca ctgaagctat gaacgaattc tcaagcgctg
     1501 caggtggttc ttgagaccag accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     1561 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
     1621 gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
     1681 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
     1741 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
     1801 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgaaacgaa
     1861 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
     1921 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
     1981 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
     2041 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc
     2101 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
     2161 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
     2221 ctcaaaatgt tcttt
//
