LOCUS       sequence-249062-        2718 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Gibberellin Receptor as a Type 3a part to be used in the MTK
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3a_020
FEATURES             Location/Qualifiers
     source          1..2718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      236..824
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     725..744
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2008..2042)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(join(2113..2718,1..54))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2687..2706
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
       61 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
      121 attatggtga aagttggaac ctcttacgtg cccgatcaat catgaccaaa atcccttaac
      181 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
      241 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
      301 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
      361 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
      421 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      481 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      541 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      601 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      661 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      721 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      781 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      841 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      901 cccctgattc tgtggataac cgtagtcggt ctcatatggc tgcgagcgat gaagttaatc
      961 ttattgagag cagaacagtg gttcctctca atacatgggt tttaatatcc aacttcaaag
     1021 tagcctacaa tatccttcgt cgccctgatg gaacctttaa ccgacactta gctgagtatc
     1081 tagaccgtaa agtcactgca aacgccaatc cggttgatgg ggttttctcg ttcgatgtct
     1141 tgattgatcg caggatcaat cttctaagca gagtctatag accagcttat gcagatcaag
     1201 agcaacctcc tagtatttta gatctcgaga agcctgttga tggcgacatt gtccctgtta
     1261 tattgttctt ccatggaggt agctttgctc attcttctgc aaacagtgcc atctacgata
     1321 ctctttgtcg caggcttgtt ggtttgtgca agtgtgttgt tgtctctgtg aattatcggc
     1381 gtgcaccaga gaatccatac ccttgtgctt atgatgatgg ttggattgct cttaattggg
     1441 ttaactcgag atcttggctt aaatccaaga aagactcaaa ggtccatatt ttcttggctg
     1501 gtgatagctc tggaggtaac atcgcgcata atgtggcttt aagagcgggt gaatcgggaa
     1561 tcgatgtttt ggggaacatt ctgctgaatc ctatgtttgg tgggaatgag agaacggagt
     1621 ctgagaaaag tttggatggg aaatactttg tgacggttag agatcgcgat tggtactgga
     1681 aagcgttttt acccgaggga gaagatagag agcatccagc gtgtaatccg tttagcccga
     1741 gagggaaaag cttagaagga gtgagtttcc ccaagagtct tgtggttgtc gcgggtttgg
     1801 atttgattag agattggcag ttggcatacg cggaagggct caagaaagcg ggtcaagagg
     1861 ttaagcttat gcatttagag aaagcaactg ttgggtttta cctcttgcct aataacaatc
     1921 atttccataa tgttatggat gagatttcgg cgtttgtaaa cgcggaatgt gaattctcaa
     1981 gcgctgcagg ttcttgagac cagaccaata aaaaacgccc ggcggcaacc gagcgttctg
     2041 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag
     2101 ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag
     2161 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
     2221 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
     2281 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgaaac
     2341 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     2401 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     2461 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     2521 tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg
     2581 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
     2641 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     2701 tgcctcaaaa tgttcttt
//
