LOCUS       sequence-249070-       11242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-based RNAi vector to clone target gene for silencing in 
            tandem with APT transcript segment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Orr RG, Foley SJ, Sherman CA, Abreu I, Galotto G, Liu B, 
            Gonzalez-Guerrero M, Vidali L
  TITLE     Robust survival-based RNAi of gene families using in tandem 
            silencing of adenine phosphoribosyltransferase.
  JOURNAL   Plant Physiol. 2020 Aug 6. pii: pp.20.00865. doi: 
            10.1104/pp.20.00865.
  PUBMED    32764132
REFERENCE   2  (bases 1 to 11242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2020 Aug 6. pii: pp.20.00865. doi: 10.1104/pp.20.00865."
COMMENT     Sequence Label: pGAPi
FEATURES             Location/Qualifiers
     source          1..11242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    2391..2514
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2555..2860)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(3202..3861)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDRVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(join(3915..3921,3922..3939,3940..3945))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(3970..4094)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    4488..4612
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4637..4642,4643..4660,4661..4667)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4721..5380
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDRVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             5722..6027
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(6068..6191)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      6611..6863
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        6896..7241
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     7184..7204
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             7271..8296
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7271..8296
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    8366..8542
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(9096..9112)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9096..9112)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9109..9131)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9120..9136
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9144..9150,9151..9168,9169..9174))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9189..9210
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9327..9344)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9498..10086)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9578..9597)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10257..11048,11049..11117))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10880..10899
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11118..11222)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctagagataa tgagcattgc atgtctaagt tataaaaaat taccacatat tttttttgtc
       61 acacttgttt gaagtgcagt ttatctatct ttatacatat atttaaactt tactctacga
      121 ataatataat ctatagtact acaataatat cagtgtttta gagaatcata taaatgaaca
      181 gttagacatg gtctaaagga caattgagta ttttgacaac aggactctac agttttatct
      241 ttttagtgtg catgtgttct cctttttttt tgcaaatagc ttcacctata taatacttca
      301 tccattttat tagtacatcc atttagggtt tagggttaat ggtttttata gactaatttt
      361 tttagtacat ctattttatt ctattttagc ctctaaatta agaaaactaa aactctattt
      421 tagttttttt atttaataat ttagatataa aatagaataa aataaagtga ctaaaaatta
      481 aacaaatacc ctttaagaaa ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga
      541 taatgccagc ctgttaaacg ccgtcgacga gtctaacgga caccaaccag cgaaccagca
      601 gcgtcgcgtc gggccaagcg aagcagacgg cacggcatct ctgtcgctgc ctctggaccc
      661 ctctcgagag ttccgctcca ccgttggact tgctccgctg tcggcatcca gaaattgcgt
      721 ggcggagcgg cagacgtgag ccggcacggc aggcggcctc ctcctcctct cacggcaccg
      781 gcagctacgg gggattcctt tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa
      841 taaatagaca ccccctccac accctctttc cccaacctcg tgttgttcgg agcgcacaca
      901 cacacaacca gatctccccc aaatccaccc gtcggcacct ccgcttcaag gtacgccgct
      961 cgtcctcccc ccccccccct ctctaccttc tctagatcgg cgttccggtc catggttagg
     1021 gcccggtagt tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg
     1081 ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac acgttctgat tgctaacttg
     1141 ccagtgtttc tctttgggga atcctgggat ggctctagcc gttccgcaga cgggatcgat
     1201 ttcatgattt tttttgtttc gttgcatagg gtttggtttg cccttttcct ttatttcaat
     1261 atatgccgtg cacttgtttg tcgggtcatc ttttcatgct tttttttgtc ttggttgtga
     1321 tgatgtggtc tggttgggcg gtcgttctag atcggagtag aattaattct gtttcaaact
     1381 acctggtgga tttattaatt ttggatctgt atgtgtgtgc catacatatt catagttacg
     1441 aattgaagat gatggatgga aatatcgatc taggataggt atacatgttg atgcgggttt
     1501 tactgatgca tatacagaga tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg
     1561 ggcggtcgtt cattcgttct agatcggagt agaatactgt ttcaaactac ctggtgtatt
     1621 tattaatttt ggaactgtat gtgtgtgtca tacatcttca tagttacgag tttaagatgg
     1681 atggaaatat cgatctagga taggtataca tgttgatgtg ggttttactg atgcatatac
     1741 atgatggcat atgcagcatc tattcatatg ctctaacctt gagtacctat ctattataat
     1801 aaacaagtat gttttataat tattttgatc ttgatatact tggatgatgg catatgcagc
     1861 agctatatgt ggattttttt agccctgcct tcatacgcta tttatttgct tggtactgtt
     1921 tcttttgtcg atgctcaccc tgttgtttgg tgttacttct gcgccagtgc caagttaatt
     1981 cgagctcaag ctttaccgtg cgccacttga cacagactgg aaatattctc gttcacacag
     2041 tgctagggct tcagggtgtg agccgtggcg catcttgtgg acttgccgtg gcgagctacc
     2101 tccacgctcc cgcttccgca attttgttgc agtgctttgg attctcgtgg ttgctcgtct
     2161 caggaagtga agatgtcggc ctccaaggat ggcgaccctc ggatccagta tatctcggac
     2221 agcatccgta ccattcctga ttttcctcac aaaggcatta tgttccgaga tgtgacgacg
     2281 ttgctgttgg atcataaggc tttcaaagac acgatcgaca tctttgttga gcgttaccgg
     2341 gaccagaagg tggacgtcat tgtgggaatt gaagctcgag gtatttaaat ccactttgta
     2401 caagaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
     2461 gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac tatggtcgac
     2521 ctgcagactg gctgtgtata agggagcctg acatttatat tccccagaac atcaggttaa
     2581 tggcgttttt gatgtcattt tcgcggtggc tgagatcagc cacttcttcc ccgataacgg
     2641 agaccggcac actggccata tcggtggtca tcatgcgcca gctttcatcc ccgatatgca
     2701 ccaccgggta aagttcacgg gagactttat ctgacagcag acgtgcactg gccaggggga
     2761 tcaccatccg tcgcccgggc gtgtcaataa tatcactctg tacatccaca aacagacgat
     2821 aacggctctc tcttttatag gtgtaaacct taaactgcat ttcaccagcc cctgttctcg
     2881 tcagcaaaag agccgttcat ttcaataaac cgggcgacct cagccatccc ttcctgattt
     2941 tccgctttcc agcgttcggc acgcagacga cgggcttcat tctgcatggt tgtgcttacc
     3001 agaccggaga tattgacatc atatatgcct tgagcaactg atagctgtcg ctgtcaactg
     3061 tcactgtaat acgctgcttc atagcatacc tctttttgac atacttcggg tatacatatc
     3121 agtatatatt cttataccgc aaaaatcagc gcgcaaatac gcatactgtt atctggcttt
     3181 tagtaagccg gatccacgcg tttacgcccc gccctgccac tcatcgcagt actgttgtaa
     3241 ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc
     3301 cagcggcatc agcaccctgt cgccttgcgt ataatatttg cccatggtga aaacgggggc
     3361 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
     3421 ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
     3481 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
     3541 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
     3601 actatcccat atcaccagct caccgtcttt cattgccata cggaattccg gatgagcatt
     3661 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
     3721 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
     3781 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
     3841 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg acggatccta
     3901 actcaaaatc cacacattat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     3961 tgcggccgcc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     4021 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttt
     4081 ttgtacaaac ttgtgattac gtacagatga acatggcatc gtggtgattg atgaaactgc
     4141 tgctgtcggc tttaacctct ctttaggcat tggtttcgaa gcgggcaaca agccgaaaga
     4201 actgtacagc gaagaggcag tcaacgggga aactcagcaa gcgcacttac aggcgattaa
     4261 agagctgata gcgcgtgaca aaaaccaccc aagcgtggtg atgtggagta ttgccaacga
     4321 accctcgagg gatacccgtc cgcaaggtgc acgggaatat ttcgcgccac tggcggaagc
     4381 aacgcgtaaa ctcgacccga cgcgtccgat cacctgcgtc aatgtaatgt tctgcgacgc
     4441 tcacaccgat accatcagcg atctctttga tgcctaggta cgtaatcaca agtttgtaca
     4501 aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc
     4561 ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggccgc
     4621 attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag
     4681 ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
     4741 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     4801 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
     4861 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
     4921 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag
     4981 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
     5041 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     5101 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
     5161 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     5221 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacagggtgc tgatgccgct
     5281 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga
     5341 attacaacag tactgcgatg agtggcaggg cggggcgtaa acgcgtggat ccggcttact
     5401 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
     5461 tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg
     5521 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
     5581 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
     5641 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
     5701 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
     5761 tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg
     5821 atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg
     5881 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc
     5941 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
     6001 attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca
     6061 ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg
     6121 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt
     6181 gtacaaagtg ggtttaaata cctcgagctt caattcccac aatgacgtcc accttctggt
     6241 cccggtaacg ctcaacaaag atgtcgatcg tgtctttgaa agccttatga tccaacagca
     6301 acgtcgtcac atctcggaac ataatgcctt tgtgaggaaa atcaggaatg gtacggatgc
     6361 tgtccgagat atactggatc cgagggtcgc catccttgga ggccgacatc ttcacttcct
     6421 gagacgagca accacgagaa tccaaagcac tgcaacaaaa ttgcggaagc gggagcgtgg
     6481 aggtagctcg ccacggcaag tccacaagat gcgccacggc tcacaccctg aagccctagc
     6541 actgtgtgaa cgagaatatt tccagtctgt gtcaagtggc gcacggtaaa gcttggtacc
     6601 cctactccaa gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
     6661 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
     6721 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
     6781 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
     6841 ggtgtcatct atgttactag atcggggata cagtctcaga agaccaaagg gctattgaga
     6901 cttttcaaca aagggtaatt tcgggaaacc tcctcggatt ccattgccca gctatctgtc
     6961 acttcatcga aaggacagta gaaaaggaag gtggctccta caaatgccat cattgcgata
     7021 aaggaaaggc tatcattcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
     7081 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
     7141 gatgtgacat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc
     7201 cttcctctat ataaggaagt tcatttcatt tggagaggac agcccaagca tggagaaccg
     7261 acctgcaggc atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
     7321 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
     7381 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
     7441 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
     7501 gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg
     7561 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga
     7621 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
     7681 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
     7741 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
     7801 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
     7861 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
     7921 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
     7981 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
     8041 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
     8101 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
     8161 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
     8221 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
     8281 gagggcaaag gaatgagcgg gactctgggg ttcggacggt acgctgaaat caccagtctc
     8341 tctctacaaa tctatctctc tctattttct ccataaataa tgtgtgagta gtttcccgat
     8401 aagggaaatt agggttctta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta
     8461 gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa
     8521 atccagtact aaaatccaga tctcctaaag tccctataga tctttgtcgt gaatataaac
     8581 cagacacgag acgactaaac ctggagccca gacgccgttc gaagctagaa gtaccgctta
     8641 ggcaggaggc cgttagggaa aagatgctaa ggcagggttg gttacgttga ctcccccgta
     8701 ggtttggttt aaatatgatg aagtggacgg aaggaaggag gaagacaagg aaggataagg
     8761 ttgcaggccc tgtgcaaggt aagaagatgg aaatttgata gaggtacgct actatactta
     8821 tactatacgc taagggaatg cttgtattta taccctatac cccctaataa ccccttatca
     8881 atttaagaaa taatccgcat aagcccccgc ttaaaaattg gtatcagagc catgaatagg
     8941 tctatgacca aaactcaaga ggataaaacc tcaccaaaat acgaaagagt tcttaactct
     9001 aaagataaaa gatctttcaa gatcaaaact agttccctca caccggtgac ggggatcgca
     9061 tgcgatatct cgagatctag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     9121 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     9181 ggtgcctaat gagtgagcta actcacatta attgcgttgt gctcactgcc cgctttccag
     9241 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     9301 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     9361 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     9421 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     9481 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     9541 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     9601 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     9661 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     9721 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9781 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9841 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9901 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     9961 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    10021 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    10081 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    10141 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    10201 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    10261 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    10321 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    10381 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    10441 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    10501 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    10561 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    10621 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    10681 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    10741 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    10801 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    10861 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    10921 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    10981 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    11041 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    11101 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    11161 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    11221 cgcacatttc cccgaaaagt gc
//
