LOCUS       sequence-249074-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX133
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(803..827)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             856..882
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             886..912
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atgggaagaa aatgttcaca ttgtggtaac actggtcaca attcaagawc ttgtagcaca
       61 ttgaaatgtg ctagtaatgg tagttttatt ggtggattaa agatttttgg agtgcaactt
      121 gatatcccta atgatgcttc atcttcttct tcttctattc acttgaagaa aagttytagt
      181 wtggagtgtt tatctccttt tgttaatgaa agttgtgaaa agagtacttc aattaataat
      241 ggttatctct ctgatggtct tgtagttcgt gttgaggaaa gaaagaaagg agttccatgg
      301 acagaggagg aacacagaaa attcttaatt ggactagaaa agctaggaaa aggagattgg
      361 agaggaattt caagaaactt tgtgacaaca aggacaccaa ctcaagttgc aagtcatgct
      421 caaaagtatt tcctaagaca atcaagtctc aacaaaaaga aacgacgttc cagccttttt
      481 gacatggcaa ggagcaacaa caagattcat gtcgatgcca taaccttgaa gttcaagcat
      541 ggagataatt ttgataattg tcttgacaca acaatcttaa caaaaaaaga ttgtcaagaa
      601 attagagcat cattattaga gttaaattca tttgttgaag atatgtcaat tgccaaagat
      661 aatcaaagga gtgaaaatcc tcaacagcct gaagagattt attcagtacc aaataagtca
      721 atttcttcat ttggagctct tgatttggag ctaagtctct cagctccaag aaatattgga
      781 ccaattacag ttacccccgg ggacccagct ttcttgtaca aagtggttga taacagcggg
      841 ttaattaaca tcttttaccc atacgatgtt cctgactatg cgggctatcc ctatgacgtc
      901 ccggactatg caggatccta tccatatgac gtwcmwgwtt amgacwrctc agtgcagcgc
      961 ttagagctcg aatttcccga tc
//
