LOCUS       sequence-249081-        2364 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes tagBFP as a Type 3a part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3a_027
FEATURES             Location/Qualifiers
     source          1..2364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             11..709
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     terminator      complement(729..763)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(834..1493)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1408..1427
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1675..2263
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2164..2183
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg
       61 caccgtggac aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg
      121 cacccagacc atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat
      181 cctggctact agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc
      241 cgacttcttc aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga
      301 agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta
      361 caacgtcaag atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac
      421 actcggctgg gaggccttca ccgaaacgct gtaccccgct gacggcggcc tggaaggcag
      481 aaacgacatg gccctgaagc tcgtgggcgg gagccatctg atcgcaaaca tcaagaccac
      541 atatagatcc aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta
      601 cagactggaa agaatcaagg aggccaacaa cgaaacctac gtcgagcagc acgaggtggc
      661 agtggccaga tactgcgacc tccctagcaa actggggcac aagcttaatg gttcttgaga
      721 ccagaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt
      781 ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc
      841 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
      901 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
      961 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     1021 aaatcaaaac tggtgaaact cacccaggga ttggctgaaa cgaaaaacat attctcaata
     1081 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
     1141 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
     1201 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
     1261 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
     1321 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
     1381 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
     1441 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
     1501 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
     1561 ttatggtgaa agttggaacc tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg
     1621 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1681 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1741 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1801 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     1861 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1921 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1981 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     2041 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2101 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2161 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2221 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2281 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2341 ccctgattct gtggataacc gtag
//
