LOCUS       sequence-249084-        1144 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1144)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX144
FEATURES             Location/Qualifiers
     source          1..1144
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(998..1022)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1051..1077
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1081..1107
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atgggtatgt ttgtagtaca tcccgcttca tttcaccacc accatcatca tcaacattca
       61 catcaagaaa ataccattaa ttttgacccg ataaacggct ccaatatctc tacaacgaac
      121 acatctcttg gtgttggtgt aattccgctt ctcactgcaa ctcctttaac gaacatggtt
      181 agcttcgacg atcaagattt agtgagtaga aatcgaggaa atgatactga aggtggtggt
      241 tttcagttct tttcaaacca acaacctcag aactctacga attataccaa aaacagtaac
      301 attcttggta gtggcaatga caacaacatt ggtggttcag tttcatcaac aagttcagca
      361 acatgtcaag attgtggaaa ccaagctaaa aaggattgtc cacacagaag atgtagaaca
      421 tgttgtaaaa gtagaggttt tgattgtaca acacatgtaa aaagtacttg ggtaccagcg
      481 gcgagaaggc gcgagagaca actaattgga ggcacttcaa cgaatgtaaa tgttgctgct
      541 ggttcatctt ctcactcaac ttcaagtgct aagaaaccta gacttgttaa ttctcaagct
      601 acaactacag cttctcatac ttctacttca aatactactc ctagaagttt tgatactagt
      661 tctagtcatc aagatgcaag ttttaaagcc tcattgccag gacaagtgag agcaccagct
      721 gtattcaagt gtgtaagagt gacttcagtg gatgatggag atgatgaatt tgcatatcaa
      781 gctgttgtta gaattggtgg ccatgttttc aaaggttttc tatatgatca aggaattgaa
      841 gaaggcaaag attataataa ttatcccaat ttatctgatt tacatttggg tggcagtggt
      901 aatattacta gtgaaacttc tgatatttat gctgcttctg ctgctggtgg tggattactt
      961 ggtggatcaa attatgttaa ccaaataaat cccggggacc cagctttctt gtacaaagtg
     1021 gttgataaca gcgggttaat taacatcttt tacccatacg atgttcctga ctatgcgggc
     1081 tatccctatg acgtcccgga ctatgcagga tcctatccat ataacgtgca aaatcaaaga
     1141 caaa
//
