LOCUS       sequence-249085-        1555 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1555)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX025
FEATURES             Location/Qualifiers
     source          1..1555
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(1406..1430)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1459..1485
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1489..1515
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atgttggatc tgaatgtatc tgcagtttct gtgaactcaa attgcgatga gactgacccc
       61 aacaacaaca ccaccctttt ctttaaagat gaatattctg ctggaacatc caacaccact
      121 gcttcctcct ccgtcgtcaa cgcccccgtc gccggtgacg aagattccaa cagctcctcc
      181 gtgcatcacg gttctaaatg ggcccaccat tctctttcaa ctctcaattt ctccatactg
      241 aagagcgatc gggccattga tactgaagat gatatgagca ctgatgacgt gctgacgagg
      301 cagctttttc cggtgaaaaa tgaatcgcag gggcagggga cgaggattca gggtcaatct
      361 cagtgctggc tgaacttgtc tgtgccggaa tccggcggcg gagcagaaat gggaatttac
      421 aaaccaccta cgccggcgca acagccggtg aagaaaagcc gacgtgggcc caggtcccgt
      481 agctcacagt atcgtggtgt cacattttac cggcgaactg gcagatggga atcgcatatc
      541 tgggattgtg ggaaacaagt gtatttggga ggatttgata ctgctcatgc tgcagctagg
      601 gcatatgatc gagctgcaat taagttccgg ggagttgatg ctgatatcaa cttcaatata
      661 actgattatg aagaagatat ggaacagatg aaaaatttgt caaaagaaga atttgttcaa
      721 gtccttcgtc gtcaaagcac tggattctct aggggaaatt caaaatacag aggggtaacc
      781 ttgcacaaat gtggtcgctg ggaggctcgt gtgggtcagt tcctaggaaa aaaggcttat
      841 gacaaggcag ccatcaaatg taatggaagg gaagcggtta ccagctttga gccaaataca
      901 tatgaacggg atatgaagga ggataataaa gatggtagtg gtcagaatct tgatctgaac
      961 ctctggattt ctccaccttt ggctggtcca aagcgtgatg aaattggtag aaacgtaccc
     1021 ttcaattttg gagctcatga aatggttatt ggaagaagat tagaggtaga gaactcctct
     1081 actgctccac aaggtgcacc aacggcttct aagctgtccg tattgactgg cgtgtatcct
     1141 ggtttttctc cgcacaatat ggaaggagca atggcgaagg ttgaagcaac ttcctctcca
     1201 ggattcccaa attgggcttg gaaaatacat agccagggtt taatcactcc agtgcccgtg
     1261 ttttcttctt ctgcagcatc atcaggattc tctaccacta ccactctgta tccgagtacc
     1321 ctcctactgc caagcaacca actacgactc cccacaaact cgactttcca acaccctccc
     1381 gtcaccctta acatcagtcc cggggaccca gctttcttgt acaaagtggt tgataacagc
     1441 gggttaatta acatctttta cccatacgat gttcctgact atgcgggcta tccctatgac
     1501 gtcccggact atgcaggatc ctatccatat actccaagac ttatactaac tacca
//
