LOCUS       sequence-249087-        3018 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes human alpha tubulin 1 as a Type 3b part to be used in the 
            MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3b_002
FEATURES             Location/Qualifiers
     source          1..3018
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2152..2186)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2257..2916)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2831..2850
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 caatcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtagt cggtctcatt
      781 ctcgtgagtg catctccatc cacgttggcc aggctggtgt ccagattggc aatgcctgct
      841 gggagctcta ctgcctggaa cacggcatcc agcccgatgg ccagatgcca agtgacaaga
      901 ccattggggg aggagatgac tccttcaaca ccttctttag tgaaacgggc gctggcaagc
      961 acgtgccccg ggctgtgttt gtagacttgg aacccacagt cattgatgaa gttcgcactg
     1021 gcacctaccg ccagctcttc caccctgagc agctcatcac aggcaaggaa gatgctgcca
     1081 ataactatgc ccgagggcac tacaccattg gcaaggagat cattgacctt gtgttggacc
     1141 gaattcgcaa gctggctgac cagtgcaccg gtcttcaggg cttcttggtt ttccacagct
     1201 ttggtggggg aactggttct gggttcacct ccctgctcat ggaacgactc tcagttgatt
     1261 atggcaagaa gtccaagctg gagttctcca tttacccagc accccaggtt tccacagctg
     1321 tagttgagcc ctacaactcc atcctcacca cccacaccac cctggagcac tctgattgtg
     1381 ccttcatggt agacaatgag gccatctatg acatctgtcg tagaaacctc gatatcgagc
     1441 gcccaaccta cactaacctt aaccgcctta ttagccagat tgtgtcctcc atcactgctt
     1501 ccctgagatt tgatggagcc ctgaatgttg acctgacaga attccagacc aacctggtgc
     1561 cctacccccg catccacttc cctctggcca catatgcccc tgtcatctct gctgagaaag
     1621 cctaccatga acagctttct gtagcagaga tcaccaatgc ttgctttgag ccagccaacc
     1681 agatggtgaa atgtgaccct cgccatggta aatacatggc ttgctgcctg ttgtaccgtg
     1741 gtgacgtggt tcccaaagat gtcaatgctg ccattgccac catcaaaacc aagcgcagca
     1801 tccagtttgt ggattggtgc cccactggct tcaaggttgg catcaactac cagcctccca
     1861 ctgtggtgcc tggtggtgac ctggccaagg tacagagagc tgtgtgcatg ctgagcaaca
     1921 ccacagccat tgctgaggcc tgggctcgcc tggaccacaa gtttgacctg atgtatgcca
     1981 agcgtgcctt tgttcactgg tacgtgggtg aggggatgga ggaaggcgag ttttcagagg
     2041 cccgtgaaga tatggctgcc cttgagaagg attatgagga ggttggtgtg gattctgttg
     2101 aaggagaggg tgaggaagaa ggagaggaat acggatcctg agaccagacc aataaaaaac
     2161 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     2221 tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc
     2281 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
     2341 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     2401 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     2461 actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt tagggaaata
     2521 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     2581 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     2641 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa
     2701 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     2761 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     2821 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     2881 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
     2941 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
     3001 acctcttacg tgcccgat
//
