LOCUS       sequence-249090-        9578 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Positive RNAi control for pGAPi-based RNAi silencing in tandem with 
            APT transcript segment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9578)
  AUTHORS   Orr RG, Foley SJ, Sherman CA, Abreu I, Galotto G, Liu B, 
            Gonzalez-Guerrero M, Vidali L
  TITLE     Robust survival-based RNAi of gene families using in tandem 
            silencing of adenine phosphoribosyltransferase.
  JOURNAL   Plant Physiol. 2020 Aug 6. pii: pp.20.00865. doi: 
            10.1104/pp.20.00865.
  PUBMED    32764132
REFERENCE   2  (bases 1 to 9578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2020 Aug 6. pii: pp.20.00865. doi: 10.1104/pp.20.00865."
COMMENT     Sequence Label: MyoUTipGAPi
FEATURES             Location/Qualifiers
     source          1..9578
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    2390..2414
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3238..3262)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    3656..3680
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4504..4528)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      4947..5199
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        5232..5577
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5520..5540
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             5607..6632
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5607..6632
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    6702..6878
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(7432..7448)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7432..7448)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7445..7467)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7456..7472
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7480..7486,7487..7504,7505..7510))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7525..7546
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7663..7680)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7834..8422)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7914..7933)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8593..9384,9385..9453))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9216..9235
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9454..9558)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctagagataa tgagcattgc atgtctaagt tataaaaaat taccacatat tttttttgtc
       61 acacttgttt gaagtgcagt ttatctatct ttatacatat atttaaactt tactctacga
      121 ataatataat ctatagtact acaataatat cagtgtttta gagaatcata taaatgaaca
      181 gttagacatg gtctaaagga caattgagta ttttgacaac aggactctac agttttatct
      241 ttttagtgtg catgtgttct cctttttttt tgcaaatagc ttcacctata taatacttca
      301 tccattttat tagtacatcc atttagggtt tagggttaat ggtttttata gactaatttt
      361 tttagtacat ctattttatt ctattttagc ctctaaatta agaaaactaa aactctattt
      421 tagttttttt atttaataat ttagatataa aatagaataa aataaagtga ctaaaaatta
      481 aacaaatacc ctttaagaaa ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga
      541 taatgccagc ctgttaaacg ccgtcgacga gtctaacgga caccaaccag cgaaccagca
      601 gcgtcgcgtc gggccaagcg aagcagacgg cacggcatct ctgtcgctgc ctctggaccc
      661 ctctcgagag ttccgctcca ccgttggact tgctccgctg tcggcatcca gaaattgcgt
      721 ggcggagcgg cagacgtgag ccggcacggc aggcggcctc ctcctcctct cacggcaccg
      781 gcagctacgg gggattcctt tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa
      841 taaatagaca ccccctccac accctctttc cccaacctcg tgttgttcgg agcgcacaca
      901 cacacaacca gatctccccc aaatccaccc gtcggcacct ccgcttcaag gtacgccgct
      961 cgtcctcccc ccccccccct ctctaccttc tctagatcgg cgttccggtc catggttagg
     1021 gcccggtagt tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg
     1081 ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac acgttctgat tgctaacttg
     1141 ccagtgtttc tctttgggga atcctgggat ggctctagcc gttccgcaga cgggatcgat
     1201 ttcatgattt tttttgtttc gttgcatagg gtttggtttg cccttttcct ttatttcaat
     1261 atatgccgtg cacttgtttg tcgggtcatc ttttcatgct tttttttgtc ttggttgtga
     1321 tgatgtggtc tggttgggcg gtcgttctag atcggagtag aattaattct gtttcaaact
     1381 acctggtgga tttattaatt ttggatctgt atgtgtgtgc catacatatt catagttacg
     1441 aattgaagat gatggatgga aatatcgatc taggataggt atacatgttg atgcgggttt
     1501 tactgatgca tatacagaga tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg
     1561 ggcggtcgtt cattcgttct agatcggagt agaatactgt ttcaaactac ctggtgtatt
     1621 tattaatttt ggaactgtat gtgtgtgtca tacatcttca tagttacgag tttaagatgg
     1681 atggaaatat cgatctagga taggtataca tgttgatgtg ggttttactg atgcatatac
     1741 atgatggcat atgcagcatc tattcatatg ctctaacctt gagtacctat ctattataat
     1801 aaacaagtat gttttataat tattttgatc ttgatatact tggatgatgg catatgcagc
     1861 agctatatgt ggattttttt agccctgcct tcatacgcta tttatttgct tggtactgtt
     1921 tcttttgtcg atgctcaccc tgttgtttgg tgttacttct gcgccagtgc caagttaatt
     1981 cgagctcaag ctttaccgtg cgccacttga cacagactgg aaatattctc gttcacacag
     2041 tgctagggct tcagggtgtg agccgtggcg catcttgtgg acttgccgtg gcgagctacc
     2101 tccacgctcc cgcttccgca attttgttgc agtgctttgg attctcgtgg ttgctcgtct
     2161 caggaagtga agatgtcggc ctccaaggat ggcgaccctc ggatccagta tatctcggac
     2221 agcatccgta ccattcctga ttttcctcac aaaggcatta tgttccgaga tgtgacgacg
     2281 ttgctgttgg atcataaggc tttcaaagac acgatcgaca tctttgttga gcgttaccgg
     2341 gaccagaagg tggacgtcat tgtgggaatt gaagctcgag gtatttaaat ccactttgta
     2401 caagaaagct gggtcggcgc gcccaccctt gcgccacgcg aaactcctga ttgcgatcac
     2461 ctcatctaca ctggtgtggc ctgcacaaga tttaaaacac gacaactcgc acgctccaca
     2521 gacggagccc ccgctccaca gcactgatcc acaaactccg cgctcactcc cccgctaatc
     2581 acctacgagc cacccctcgc ccgcctcctc cctcctgatc cagcaaatat tgcaacgcac
     2641 ggtctatctg accgcttcca cggcgagaaa aataaaaata aaagtaaaaa aaaaatgccc
     2701 gggcttgatg atgttccttg agaaacaatc tgcgcgcctc tccgcggctc cgagagggac
     2761 cgaggcgaag tattgattcg agtcagtgtg gagagcagat gcggagggga gggaggggaa
     2821 gggggacagc actaacatta cgggtgaagg gggcggccgc gggaattcga ttctttttca
     2881 attgtgattt cgcgttggtt gtgttggtgc tgtctcctcc tcctcctcgt catctcctct
     2941 tcagacgcac agtcgaagct acaccccccc ccccaattac tgtggttggc tggcacctag
     3001 gatcacgcac gcacgctctc taccgggtct tcatcgacac tttcatttct cccaggctag
     3061 gagaggtttt gttctttgct gtggaagtgt gctgctgccg ctgctcaggg gaggggctgc
     3121 tgctgtgagt agcaagattc gttagattac gaacgaggac gacgacgagg aacacgaggg
     3181 gacgctttct tggtgattgg gacgggggtt caatcactag tgaattcgcg gccgcggagc
     3241 ctgctttttt gtacaaactt gtgattacgt acagatgaac atggcatcgt ggtgattgat
     3301 gaaactgctg ctgtcggctt taacctctct ttaggcattg gtttcgaagc gggcaacaag
     3361 ccgaaagaac tgtacagcga agaggcagtc aacggggaaa ctcagcaagc gcacttacag
     3421 gcgattaaag agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt
     3481 gccaacgaac cctcgaggga tacccgtccg caaggtgcac gggaatattt cgcgccactg
     3541 gcggaagcaa cgcgtaaact cgacccgacg cgtccgatca cctgcgtcaa tgtaatgttc
     3601 tgcgacgctc acaccgatac catcagcgat ctctttgatg cctaggtacg taatcacaag
     3661 tttgtacaaa aaagcaggct ccgcggccgc gaattcacta gtgattgaac ccccgtccca
     3721 atcaccaaga aagcgtcccc tcgtgttcct cgtcgtcgtc ctcgttcgta atctaacgaa
     3781 tcttgctact cacagcagca gcccctcccc tgagcagcgg cagcagcaca cttccacagc
     3841 aaagaacaaa acctctccta gcctgggaga aatgaaagtg tcgatgaaga cccggtagag
     3901 agcgtgcgtg cgtgatccta ggtgccagcc aaccacagta attggggggg ggggtgtagc
     3961 ttcgactgtg cgtctgaaga ggagatgacg aggaggagga ggagacagca ccaacacaac
     4021 caacgcgaaa tcacaattga aaaagaatcg aattcccgcg gccgccccct tcacccgtaa
     4081 tgttagtgct gtcccccttc ccctccctcc cctccgcatc tgctctccac actgactcga
     4141 atcaatactt cgcctcggtc cctctcggag ccgcggagag gcgcgcagat tgtttctcaa
     4201 ggaacatcat caagcccggg catttttttt ttacttttat ttttattttt ctcgccgtgg
     4261 aagcggtcag atagaccgtg cgttgcaata tttgctggat caggagggag gaggcgggcg
     4321 aggggtggct cgtaggtgat tagcggggga gtgagcgcgg agtttgtgga tcagtgctgt
     4381 ggagcggggg ctccgtctgt ggagcgtgcg agttgtcgtg ttttaaatct tgtgcaggcc
     4441 acaccagtgt agatgaggtg atcgcaatca ggagtttcgc gtggcgcaag ggtgggcgcg
     4501 ccgacccagc tttcttgtac aaagtgggtt taaatacctc gagcttcaat tcccacaatg
     4561 acgtccacct tctggtcccg gtaacgctca acaaagatgt cgatcgtgtc tttgaaagcc
     4621 ttatgatcca acagcaacgt cgtcacatct cggaacataa tgcctttgtg aggaaaatca
     4681 ggaatggtac ggatgctgtc cgagatatac tggatccgag ggtcgccatc cttggaggcc
     4741 gacatcttca cttcctgaga cgagcaacca cgagaatcca aagcactgca acaaaattgc
     4801 ggaagcggga gcgtggaggt agctcgccac ggcaagtcca caagatgcgc cacggctcac
     4861 accctgaagc cctagcactg tgtgaacgag aatatttcca gtctgtgtca agtggcgcac
     4921 ggtaaagctt ggtaccccta ctccaagatc gttcaaacat ttggcaataa agtttcttaa
     4981 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta
     5041 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
     5101 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
     5161 ataaattatc gcgcgcggtg tcatctatgt tactagatcg gggatacagt ctcagaagac
     5221 caaagggcta ttgagacttt tcaacaaagg gtaatttcgg gaaacctcct cggattccat
     5281 tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaaggtgg ctcctacaaa
     5341 tgccatcatt gcgataaagg aaaggctatc attcaagatg cctctgccga cagtggtccc
     5401 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
     5461 tcaaagcaag tggattgatg tgacatctcc actgacgtaa gggatgacgc acaatcccac
     5521 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacagcc
     5581 caagcatgga gaaccgacct gcaggcatga aaaagcctga actcaccgcg acgtctgtcg
     5641 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg
     5701 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
     5761 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
     5821 tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct
     5881 cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc
     5941 tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg
     6001 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat
     6061 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
     6121 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc
     6181 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa
     6241 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca
     6301 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga
     6361 ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg
     6421 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc
     6481 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
     6541 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac
     6601 gccccagcac tcgtccgagg gcaaaggaat gagcgggact ctggggttcg gacggtacgc
     6661 tgaaatcacc agtctctctc tacaaatcta tctctctcta ttttctccat aaataatgtg
     6721 tgagtagttt cccgataagg gaaattaggg ttcttatagg gtttcgctca tgtgttgagc
     6781 atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct
     6841 aattcctaaa accaaaatcc agtactaaaa tccagatctc ctaaagtccc tatagatctt
     6901 tgtcgtgaat ataaaccaga cacgagacga ctaaacctgg agcccagacg ccgttcgaag
     6961 ctagaagtac cgcttaggca ggaggccgtt agggaaaaga tgctaaggca gggttggtta
     7021 cgttgactcc cccgtaggtt tggtttaaat atgatgaagt ggacggaagg aaggaggaag
     7081 acaaggaagg ataaggttgc aggccctgtg caaggtaaga agatggaaat ttgatagagg
     7141 tacgctacta tacttatact atacgctaag ggaatgcttg tatttatacc ctataccccc
     7201 taataacccc ttatcaattt aagaaataat ccgcataagc ccccgcttaa aaattggtat
     7261 cagagccatg aataggtcta tgaccaaaac tcaagaggat aaaacctcac caaaatacga
     7321 aagagttctt aactctaaag ataaaagatc tttcaagatc aaaactagtt ccctcacacc
     7381 ggtgacgggg atcgcatgcg atatctcgag atctagcttg gcgtaatcat ggtcatagct
     7441 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     7501 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgtgctc
     7561 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     7621 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     7681 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     7741 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     7801 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     7861 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     7921 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     7981 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     8041 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     8101 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8161 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8221 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     8281 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     8341 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     8401 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     8461 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     8521 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     8581 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     8641 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     8701 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     8761 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     8821 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     8881 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     8941 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9001 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     9061 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     9121 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     9181 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     9241 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9301 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     9361 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     9421 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     9481 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     9541 acaaataggg gttccgcgca catttccccg aaaagtgc
//
