LOCUS       sequence-249096-        4159 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  asymmetric plasmid partitioning E. coli - regulatory plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Molinari S, Shis DL, Bhakta SP, Chappell J, Igoshin OA, Bennett MR
  TITLE     A synthetic system for asymmetric cell division in Escherichia coli.
  JOURNAL   Nat Chem Biol. 2019 Sep;15(9):917-924. doi: 
            10.1038/s41589-019-0339-x. Epub 2019 Aug 12.
  PUBMED    31406375
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0339-x"; journalName: "Nat Chem Biol"; date: 
            "2019-09"; volume: "15"; issue: "9"; pages: "917-924"
COMMENT     Sequence Label: pSPB862
FEATURES             Location/Qualifiers
     source          1..4159
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             50..61
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    108..129
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             311..319
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    1010..1066
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    2031..2051
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(join(2279..3070,3071..3139))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2902..2921
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3140..3244)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(3288..3319)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     primer_bind     complement(3463..3480)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3597..4142)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(3677..3696)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acggaaaaaa aaaccccgcc cctgacaggg cggggttttt tttccaatta ttgaaggccg
       61 ctaacgcggc ctttttttgt ttctggtctg ccttaatcaa tgactaagaa ttcgcggccg
      121 cttctagagc gtctcacttc ggatccgctg caggcttccg cacccatagc atttttatcc
      181 ataagattag cggatcctac cttttacttc ttgccatcga ctacggatac tgagcacgac
      241 gggcccaagt tcacttaaaa aggagatcaa caatgaaagc aattttcgta ctgaaacatc
      301 ttaatcatgc taaggaggtt ttctaatgga taatacagaa gatgttatta aagaatttat
      361 gcgttttaaa gttcgtatgg aaggttcagt taatggtcat gaatttgaaa ttgaaggtga
      421 aggtgaagga cgtccatatg aaggtacaca aacagcaaaa ttaaaagtta caaaaggtgg
      481 tccattacca tttgcatggg atattttatc accacaattt caatatggtt caaaagttta
      541 tgttaaacat ccagcagata ttccagatta taagaaatta tcatttcctg aaggttttaa
      601 atgggaacgt gttatgaatt ttgaagatgg tggtgttgtt acagttacac aagattcatc
      661 attacaagat ggttgtttta tctataaagt taaatttatt ggtgttaatt ttccatcaga
      721 tggtccagtt atgcaaaaga aaacaatggg ttgggaacca tcaacagaac gtttatatcc
      781 acgtgatggt gttttaaaag gtgaaattca taaagcattg aaattaaaag atggtggtca
      841 ttatttagtt gaatttaaat caatttatat ggcaaaaaaa ccagttcaat taccaggtta
      901 ttattatgtt gattcaaaat tagatattac atcacataat gaagattata caattgttga
      961 acaatatgaa cgtacagaag gtcgtcatca tttgttttta ggttccaaga gatctcgagc
     1021 tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccatgt ccgaagggcg
     1081 tcgtggtctg ggtcgagggc tttcggccct gctgggcgag gtcgacgccg cgccggctca
     1141 ggcgccgggc gagcagcttg gcggttcccg tgaagcgccg atcgaaatcc tgcagcgcaa
     1201 tcccgaccag ccccgtcgga ccttccgcga ggaagacctc gaggatctgt caaactcgat
     1261 ccgtgagaag ggcgttctgc agccgatcct ggtgcggccg tcgccggata cggctggcga
     1321 gtaccagatc gtcgccggcg agcgccgttg gcgcgcagcc cagcgggcgg gcctcaagac
     1381 tgtgccgatc atggtgcgcg agctcgacga cctggcggtg ctggagatcg gcatcatcga
     1441 gaacgtccag cgggctgatc tgaacgtcct ggaagaagcg ctctcctaca aggtgctgat
     1501 ggagaagttc gagcgcaccc aggagaacat cgcccagacc atcggcaaga gccgcagcca
     1561 cgtggccaac acgatgcgct tgctggccct accggacgag gttcagtcgc acctggtcag
     1621 tggtgagctg acggccggcc acgcccgcgc catcgctgca gcggctgacc cggtggccct
     1681 cgccaagcaa atcatcgagg gcggcttgtc ggtgcgcgag acagaggccc tggcccgtaa
     1741 ggcgcccaac ctgtctgcgg gcaagagcaa ggggggccgc cccccacgcg tgaaggacac
     1801 cgacacccag gcgctggagt ctgacctgtc ctcggttctg ggtctggatg tctcgatcga
     1861 ccatcgcggg tctaccggca cgctgaccat cacctacgcc accctcgagc agctcgacga
     1921 cctctgcaac cgactgacgc gcgggatcta ataatggcct cggtaccaaa gacgaacaat
     1981 aagacgctga aaagcgtctt ttttcgtttt ggtccgctgc gtttgagacg tactagtagc
     2041 ggccgctgca gttaatcact gattaactcg gtaccaaatt ccagaaaaga ggcctcccga
     2101 aaggggggcc ttttttcgtt ttggtccttt tgttatcaat aaaaaagggg agcggtttcc
     2161 cgctcccctt attgttcgtc tacagattat caaaaaggat cttcacctag atccttttaa
     2221 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     2281 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     2341 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     2401 gtgctgcaat gataccgcgg gacccacgct caccggctcc agatttatca gcaataaacc
     2461 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2521 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2581 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2641 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2701 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2761 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     2821 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     2881 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     2941 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     3001 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     3061 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     3121 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     3181 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3241 cgcgcacatt tccccgaaaa gtgccacctg gagtcctgtt gagtaatagt caaaagcctc
     3301 cggtcggagg cttttgactt tctgcttacc caatagcgga gtgtatactg gcttactatg
     3361 ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc
     3421 ggtgcgtcag cagaatatgt gatacaggat atattccgct tcctcgctca ctgactcgct
     3481 acgctcggtc gttcgactgc ggcgagcgga aatggcttac gaacggggcg gagatttcct
     3541 ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc
     3601 cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga
     3661 aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct cgtgcgctct
     3721 cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg tttgtctcat
     3781 tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga
     3841 accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3901 ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta gaggagttag
     3961 tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt gactgcgctc
     4021 ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc
     4081 cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca aaacgatctc
     4141 aagaagatca tcttattaa
//
