LOCUS       sequence-249103-        2439 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes mScarlet as a Type 3b part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3b_010
FEATURES             Location/Qualifiers
     source          1..2439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(804..838)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(909..1568)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1483..1502
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1750..2338
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2239..2258
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat tctgtatcaa aaggcgaggc ggtcattaaa gagttcatga ggtttaaagt
       61 ccatatggaa ggaagcatga atggtcacga gtttgagata gagggagagg gtgagggaag
      121 gccgtacgaa gggacgcaaa ctgcgaagtt gaaggtaacg aaaggcggcc ctctgccatt
      181 ctcttgggac atcctttcac cgcaatttat gtacggctct agggccttca caaaacaccc
      241 cgccgatatt ccggattact ataaacaaag ttttcctgag ggcttcaagt gggaaagagt
      301 aatgaacttc gaggatgggg gagctgtgac ggttactcaa gacactagcc tggaggacgg
      361 tactcttata tacaaggtaa aactaagagg gacgaatttt ccgccagacg gccccgtgat
      421 gcaaaaaaaa actatggggt gggaagcatc caccgagagg ctgtaccccg aagacggggt
      481 cctgaagggt gatattaaaa tggcattaag attaaaagat ggcgggagat acttagctga
      541 cttcaagact acatataaag ccaaaaaacc tgtacagatg cctggagctt acaatgtgga
      601 tcgtaagctg gatattacat cccacaacga agattatacc gtggtggagc agtacgagcg
      661 ttcagagggg cgtcactcca ccgggggtat ggatgaatta tacaagagcg gtcttagaag
      721 cagggcgcaa gcgtcaaatt cagcggtaga tggaacagca ggtccaggta gtactggcag
      781 cagaggatcc tgagaccaga ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca
      841 aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg
      901 atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
      961 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     1021 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     1081 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgaaacgaaa
     1141 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     1201 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     1261 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     1321 accagctcac cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca
     1381 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     1441 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     1501 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     1561 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     1621 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgcccgat caatcatgac
     1681 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1741 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1801 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1861 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1921 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1981 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2041 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2101 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2161 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2221 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2281 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2341 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2401 ctttcctgcg ttatcccctg attctgtgga taaccgtag
//
