LOCUS       sequence-249106-        2613 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes iRFP713 as a Type 3b part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3b_011
FEATURES             Location/Qualifiers
     source          1..2613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     100..119
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1278..1312)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1383..2042)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1957..1976
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(2224..2613,1..199)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
       61 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      121 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      181 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      241 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtag
      301 tcggtctcat tctgctgaag gttctgttgc tagacaacca gacttgttga cttgtgacga
      361 cgaaccaatc cacatcccag gtgctatcca accacacggt ttgttgttgg ctttggctgc
      421 tgacatgact atcgttgctg gttctgacaa cttgccagaa ttgactggtt tggctatcgg
      481 tgctttgatc ggtagatctg ctgctgacgt tttcgactct gaaactcaca acagattgac
      541 tatcgctttg gctgaaccag gtgctgctgt tggtgctcca atcactgttg gtttcactat
      601 gagaaaggac gctggtttca tcggttcttg gcacagacac gaccaattga tcttcttgga
      661 attggaacca ccacaaagag atgttgctga accacaagct ttcttcagaa gaactaactc
      721 tgctatcaga agattgcaag ctgctgaaac tttggaatct gcttgtgctg ctgctgctca
      781 agaagttaga aagatcactg gtttcgacag agttatgatc tacagattcg cttctgactt
      841 ctctggtgaa gttatcgctg aagacagatg tgctgaagtt gaatctaagt tgggtttgca
      901 ctacccagct tctactgttc cagctcaagc tagaagattg tacactatca acccagttag
      961 aatcatccca gacatcaact acagaccagt tccagttact ccagacttga acccagttac
     1021 tggtagacca atcgacttgt ctttcgctat cttgagatct gtttctccag ttcacttgga
     1081 attcatgaga aacatcggta tgcacggtac tatgtctatc tctatcttga gaggtgaaag
     1141 attgtggggt ttgatcgttt gtcaccacag aactccatac tacgttgact tggacggtag
     1201 acaagcttgt gaattggttg ctcaagtttt ggcttggcaa atcggtgtta tggaagaagg
     1261 atcctgagac cagaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca
     1321 gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca
     1381 aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg
     1441 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
     1501 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg
     1561 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgaaac gaaaaacata
     1621 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc
     1681 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
     1741 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
     1801 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg
     1861 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
     1921 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
     1981 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
     2041 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     2101 cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaatca tgaccaaaat
     2161 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2221 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     2281 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     2341 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     2401 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     2461 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     2521 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     2581 gacctacacc gaactgagat acctacagcg tga
//
