LOCUS       sequence-249109-        4154 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  asymmetric plasmid partitioning E. coli - regulatory plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Molinari S, Shis DL, Bhakta SP, Chappell J, Igoshin OA, Bennett MR
  TITLE     A synthetic system for asymmetric cell division in Escherichia coli.
  JOURNAL   Nat Chem Biol. 2019 Sep;15(9):917-924. doi: 
            10.1038/s41589-019-0339-x. Epub 2019 Aug 12.
  PUBMED    31406375
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0339-x"; journalName: "Nat Chem Biol"; date: 
            "2019-09"; volume: "15"; issue: "9"; pages: "917-924"
COMMENT     Sequence Label: pSPB863
FEATURES             Location/Qualifiers
     source          1..4154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             50..61
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    108..129
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    1005..1061
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    2026..2046
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(join(2274..3065,3066..3134))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2897..2916
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3135..3239)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(3283..3314)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     primer_bind     complement(3458..3475)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3592..4137)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(3672..3691)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acggaaaaaa aaaccccgcc cctgacaggg cggggttttt tttccaatta ttgaaggccg
       61 ctaacgcggc ctttttttgt ttctggtctg ccttaatcaa tgactaagaa ttcgcggccg
      121 cttctagagc gtctcacttc ggatccgctg caggcttccg cacccatagc atttttatcc
      181 ataagattag cggatcctac cttttacttc ttgccatcga ctacggatac tgagcacgac
      241 atgcatgcca cttaaaaagg agatcaacaa tgaaagcaat tttcgtactg aaacatctta
      301 atcatgctaa ggagacttca atggataata cagaagatgt tattaaagaa tttatgcgtt
      361 ttaaagttcg tatggaaggt tcagttaatg gtcatgaatt tgaaattgaa ggtgaaggtg
      421 aaggacgtcc atatgaaggt acacaaacag caaaattaaa agttacaaaa ggtggtccat
      481 taccatttgc atgggatatt ttatcaccac aatttcaata tggttcaaaa gtttatgtta
      541 aacatccagc agatattcca gattataaga aattatcatt tcctgaaggt tttaaatggg
      601 aacgtgttat gaattttgaa gatggtggtg ttgttacagt tacacaagat tcatcattac
      661 aagatggttg ttttatctat aaagttaaat ttattggtgt taattttcca tcagatggtc
      721 cagttatgca aaagaaaaca atgggttggg aaccatcaac agaacgttta tatccacgtg
      781 atggtgtttt aaaaggtgaa attcataaag cattgaaatt aaaagatggt ggtcattatt
      841 tagttgaatt taaatcaatt tatatggcaa aaaaaccagt tcaattacca ggttattatt
      901 atgttgattc aaaattagat attacatcac ataatgaaga ttatacaatt gttgaacaat
      961 atgaacgtac agaaggtcgt catcatttgt ttttaggttc caagagatct cgagctcaag
     1021 cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc catgtccgaa gggcgtcgtg
     1081 gtctgggtcg agggctttcg gccctgctgg gcgaggtcga cgccgcgccg gctcaggcgc
     1141 cgggcgagca gcttggcggt tcccgtgaag cgccgatcga aatcctgcag cgcaatcccg
     1201 accagccccg tcggaccttc cgcgaggaag acctcgagga tctgtcaaac tcgatccgtg
     1261 agaagggcgt tctgcagccg atcctggtgc ggccgtcgcc ggatacggct ggcgagtacc
     1321 agatcgtcgc cggcgagcgc cgttggcgcg cagcccagcg ggcgggcctc aagactgtgc
     1381 cgatcatggt gcgcgagctc gacgacctgg cggtgctgga gatcggcatc atcgagaacg
     1441 tccagcgggc tgatctgaac gtcctggaag aagcgctctc ctacaaggtg ctgatggaga
     1501 agttcgagcg cacccaggag aacatcgccc agaccatcgg caagagccgc agccacgtgg
     1561 ccaacacgat gcgcttgctg gccctaccgg acgaggttca gtcgcacctg gtcagtggtg
     1621 agctgacggc cggccacgcc cgcgccatcg ctgcagcggc tgacccggtg gccctcgcca
     1681 agcaaatcat cgagggcggc ttgtcggtgc gcgagacaga ggccctggcc cgtaaggcgc
     1741 ccaacctgtc tgcgggcaag agcaaggggg gccgcccccc acgcgtgaag gacaccgaca
     1801 cccaggcgct ggagtctgac ctgtcctcgg ttctgggtct ggatgtctcg atcgaccatc
     1861 gcgggtctac cggcacgctg accatcacct acgccaccct cgagcagctc gacgacctct
     1921 gcaaccgact gacgcgcggg atctaataat ggcctcggta ccaaagacga acaataagac
     1981 gctgaaaagc gtcttttttc gttttggtcc gctgcgtttg agacgtacta gtagcggccg
     2041 ctgcagttaa tcactgatta actcggtacc aaattccaga aaagaggcct cccgaaaggg
     2101 gggccttttt tcgttttggt ccttttgtta tcaataaaaa aggggagcgg tttcccgctc
     2161 cccttattgt tcgtctacag attatcaaaa aggatcttca cctagatcct tttaaattaa
     2221 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     2281 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     2341 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     2401 gcaatgatac cgcgggaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     2461 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     2521 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     2581 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     2641 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     2701 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     2761 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     2821 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     2881 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     2941 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     3001 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     3061 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     3121 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     3181 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     3241 acatttcccc gaaaagtgcc acctggagtc ctgttgagta atagtcaaaa gcctccggtc
     3301 ggaggctttt gactttctgc ttacccaata gcggagtgta tactggctta ctatgttggc
     3361 actgatgagg gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct gcaccggtgc
     3421 gtcagcagaa tatgtgatac aggatatatt ccgcttcctc gctcactgac tcgctacgct
     3481 cggtcgttcg actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag
     3541 atgccaggaa gatacttaac agggaagtga gagggccgcg gcaaagccgt ttttccatag
     3601 gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc
     3661 gacaggacta taaagatacc aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt
     3721 tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac
     3781 gcctgacact cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc
     3841 ccgttcagtc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa
     3901 gacatgcaaa agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg
     3961 aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca
     4021 agccagttac ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc
     4081 aaggcggttt tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa
     4141 gatcatctta ttaa
//
