LOCUS       sequence-249113-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  polyhistidine replacement of EatA passenger DUF corresponding to 
            amino acids E541 -Q617 of the native EatA protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Sheikh A, Wangdi T, Vickers TJ, Aaron B, Palmer M, Miller MJ, Kim S,
            Herring C, Simoes R, Crainic JA, Gildersleeve JC, van der Post S, 
            Hansson GC, Fleckenstein JM
  TITLE     Enterotoxigenic Escherichia coli degrades the host MUC2 mucin 
            barrier to facilitate critical pathogen-enterocyte interactions in 
            human small intestine.
  JOURNAL   Infect Immun. 2021 Nov 22:IAI0057221. doi: 10.1128/IAI.00572-21.
  PUBMED    34807735
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun. 2021 Nov 22:IAI0057221. doi: 10.1128/IAI.00572-21."
COMMENT     Sequence Label: pTW5126
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             338..364
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
ORIGIN
        1 ttggtgaggc acattaactg ttaatggaaa aggggttaat aatggaggac tgaaagtcgg
       61 tgatggaacc gtaattctga atcaacgccc tgatgataat ggacacaagc aagcctttag
      121 ctctattaac atttccagtg gtcgtgcaac agttatactt tcagatgcta atcaagttaa
      181 cccagataaa atatcatggg gatatagagg cggtactctt gatttaaatg gaaataatgt
      241 aaactttact cgtcttcagg ctgcagatta tggtgctatt gtttctaaca ataacaaaaa
      301 caaatctgaa ttaacactta aattacaaac actaaatcat catcatcatc atcatcatca
      361 tcatctcgag caaccttata tgtttcatgg acaacttaat ggttatatgg atgtaaatat
      421 acatccactc tctggtaagg atgtgctgac tcttgatggt tctgttaatc tgcccgaagg
      481 ggtgataacg aaaaagtcag gtactctgat atttcaaggg catccggtga ttcatgctgg
      541 aatgacaacc tcagccggcc agagtgattg ggaaaatcgt cagtttacaa tggataaact
      601 gaggcttgat gcagcaacat tccatctctc cagaaatgct catatgcagg gagatattag
      661 tgctgccaac ggaagcaccg tcattctggg aagttctcgg gtctttactg acaagaatga
      721 cggaaccggt aatgcggcat cttctgttga agggagttcc attgcaacaa cagctggtga
      781 ccaaagttat tacagcggta atgtgctgct ggaaaaccat tcgtctctag aggtcaggna
      841 gaattttact ggtggtattg aggcttatga cagttctgtt agtgtgacct ctcanaatgc
      901 tatttttgac catgttggta gctttgttaa cagtagtctg cttctcgaaa aaggagcaaa
      961 actgacagca cagagt
//
