LOCUS       sequence-249117-        2886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Flamindo2,a cAMP reporter, as a Type 3 part to be used in 
            the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3b_013
FEATURES             Location/Qualifiers
     source          1..2886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(825..846)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1086..1105)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             1749..2000
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     1937..1958
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      complement(2020..2054)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2125..2784)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2699..2718
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 caatcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtagt cggtctcatt
      781 ctgtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      841 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      901 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      961 tcgtgaccac cttcggctac ggcctgatgt gcttcgcccg ctaccccgac cacatgaagc
     1021 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     1081 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     1141 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     1201 agctggagta caacctccgc ggggcactga agaaagagga gcttgttgag gctatggccc
     1261 tcctgtccca gcgagggcct gatgccctac tcaccgtggc gctccggaag cccccaggtc
     1321 agcgtacgga tgaagaactg gacctgatct ttgaagagct gctgcatatc aaggcggtgg
     1381 cacacctttc taactcggtg aagcgggaac tagctgctgt tctgctcttt gaaccacaca
     1441 gcaaggcagg aactgtgttg ttcagccagg gggacaaggg tacctcgtgg tacattatct
     1501 ggaagggatc tgtcaatgtg gtgacccatg gcaaggggct ggtgaccacg ttgcacgagg
     1561 gagatgactt tggacagctg gctctggtga acgacgcacc tcgggcagcc accatcatcc
     1621 ttcgagaaaa taactgtcac tttctgcgtg tggacaagca ggacttcaac cgcatcatca
     1681 aggatgtgga agcaaaaacc atgagactgg aagaagaatt caacagccac aacgtctata
     1741 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     1801 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     1861 ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca
     1921 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     1981 gcatggacga gctgtacaag ggatcctgag accagaccaa taaaaaacgc ccggcggcaa
     2041 ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg
     2101 agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg
     2161 taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat
     2221 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
     2281 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
     2341 attggctgaa acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
     2401 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
     2461 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
     2521 aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc
     2581 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
     2641 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
     2701 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
     2761 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     2821 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     2881 cccgat
//
