LOCUS       sequence-249121-        2385 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes rat Connexin 26 as a Type 3b part to be used in the MTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3b_015
FEATURES             Location/Qualifiers
     source          1..2385
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1519..1553)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1624..2283)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2198..2217
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 caatcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtagt cggtctcatt
      781 ctggactccg ctctcgtgct caggattggg gcacactaca gagcatcctc gggggtgtca
      841 acaagcactc caccagcatt gggaaaatct ggctcactgt cctcttcatc ttccgcatca
      901 tgatcctcgt ggtggccgcg aaggaggtgt ggggagatga gcaagccgat tttgtttgca
      961 acactctcca gcctggctgt aagaatgtgt gctacgacca ctacttcccc atctctcaca
     1021 tccggctctg ggctctgcag ctgatcatgg tgtccacgcc ggccctcctg gtagctatgc
     1081 acgtggccta ccggagacac gaaaagaaac ggaagttcat gaagggagag ataaagaacg
     1141 agtttaagga catcgaagag atcaaaaccc agaaggtccg tatcgaaggg tccctgtggt
     1201 ggacctacac caccagcatc ttcttccggg tcatcttcga agctgtcttc atgtatgtct
     1261 tttacatcat gtacaatggc ttcttcatgc agcgtctggt gaagtgtaac gcctggcctt
     1321 gtcccaatac agtggactgc ttcatttcca ggcccacaga aaagactgtc ttcacggtgt
     1381 tcatgatctc tgtgtctgga atttgcatcc tgctaaacat cacagagctg tgctatctgt
     1441 tcattaggta ttgctcaggg aagtccaaaa gaccagtcgc ggatccaccg gtcgccaccg
     1501 gatcctgaga ccagaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
     1561 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc
     1621 aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc
     1681 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt
     1741 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt
     1801 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaaa cgaaaaacat
     1861 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg
     1921 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa
     1981 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
     2041 ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat
     2101 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
     2161 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
     2221 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
     2281 cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga
     2341 tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgat
//
