LOCUS       sequence-249124-        2493 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes human Connexin 30 as a Type 3b part to be used in the MTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3b_016
FEATURES             Location/Qualifiers
     source          1..2493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1627..1661)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1732..2391)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2306..2325
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 caatcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtagt cggtctcatt
      781 ctggactccg ctctcgtgct caggattggg ggacgctgca cactttcatc gggggtgtca
      841 acaaacactc caccagcatc gggaaggtgt ggatcacagt catctttatt ttccgagtca
      901 tgatcctcgt ggtggctgcc caggaagtgt ggggtgacga gcaagaggac ttcgtctgca
      961 acacactgca accgggatgc aaaaatgtgt gctatgacca ctttttcccg gtgtcccaca
     1021 tccggctgtg ggccctccag ctgatctttg tctccacccc agcgctgctg gtggccatgc
     1081 atgtggccta ctacaggcac gaaaccactc gcaagttcag gcgaggagag aagaggaatg
     1141 atttcaaaga catagaggac attaaaaagc agaaggttcg gatagagggg tcgctgtggt
     1201 ggacgtacac cagcagcatc tttttccgaa tcatctttga agcagccttt atgtatgtgt
     1261 tttacttcct ttacaatggg taccacctgc cctgggtgtt gaaatgtggg attgacccct
     1321 gccccaacct tgttgactgc tttatttcta ggccaacaga gaagaccgtg tttaccattt
     1381 ttatgatttc tgcgtctgtg atttgcatgc tgcttaacgt ggcagagttg tgctacctgc
     1441 tgctgaaagt gtgttttagg agatcaaaga gagcacagac gcaaaaaaat caccccaatc
     1501 atgccctaaa ggagagtaag cagaatgaaa tgaatgagct gatttcagat agtggtcaaa
     1561 atgcaatcac aggtttccca agctacccgg atccaccggt cgccaccgga tcctgagacc
     1621 agaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
     1681 gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc
     1741 gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
     1801 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
     1861 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
     1921 atcaaaactg gtgaaactca cccagggatt ggctgaaacg aaaaacatat tctcaataaa
     1981 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
     2041 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg
     2101 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
     2161 cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
     2221 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
     2281 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
     2341 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
     2401 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
     2461 atggtgaaag ttggaacctc ttacgtgccc gat
//
